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Sridevi Anggraini
Universitas Singaperbangsa Karawang (UNSIKA) Jawa Barat, Indonesia
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Isolasi dan Identifikasi Senyawa Flavonoid pada Tanaman Audi Ichsani; Christina Febiola Lubis; Lestari Mahardika Urbaningrum; Nurma Dwi Rahmawati; Sridevi Anggraini
Jurnal Health Sains Vol. 2 No. 6 (2021): Jurnal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v2i6.188

Abstract

Isolation of flavonoid compounds can be done in various stages. In this study, a literature study was done to determine the procedure for the Isolation and Identification of Flavonoid Compounds. The procedure usually begins with a phytochemical test (screening) first. Flavonoid phytochemical tests can be done with various types of tests, Bate Smith-Matcalfe test, 10% NaOH test, and Wilstatter test. It can be done with all of the tests or just one of them. The first step is extraction with a suitable solvent. The suitable solvents for the isolation of flavonoid compounds are polar and semi-polar solvents. Extraction of plant compounds for isolated flavonoid compounds used the maceration method. Maceration carried out for 72 hours while stirring every 24 hours. After that the extract was concentrated with a rotary vacuum evaporator. The next step is fractionation. For the fractionation of isolated flavonoid compounds, it can be done by the methods of Liquid-Liquid Extraction, Vacuum Liquid Chromatography, Column Chromatography, and Thin Layer Chromatography. Solvents that can be used in this step vary, depending on the method. Then, isolate be monitored to determine the type of flavonoid contained in the isolate. The methods that can be used in this step are UV-Vis Spectrophotometer, HNMR, and FTIR spectrophotometer. You can use one method or two methods.
Analisis Senyawa Paracetamol (Acetaminophen) dalam Sampel Urin Menggunakan Metode Kromatografi dan Spektrometri Dianti Pratiwi; Dila Qhoirul Nisa; Elsya Martia; Ivan Iduljana; Nurma Dwi Rahmawati; Sridevi Anggraini
Jurnal Health Sains Vol. 3 No. 4 (2022): Jurnal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v3i4.473

Abstract

Paracetamol (acetaminophen) is a drug that has an analgesic-antipyretic effect. Research has been carried out with the aim of analyzing the compound paracetamol (acetaminophen) in urine qualitatively using Gas Chrotography-Mass Spectrometry (CG-MS) and LC-MS (Liquid Chromathography – Mass Spectrometry). Urine samples were obtained from patients taking paracetamol without changing consumption patterns. The results of the research analysis showed that positive results of acetaminophen-TMS were obtained in urine samples from 1 hour to a day after taking paracetamol tablets but negative results were obtained after more than 1 day of taking tablets.
Co-Authors Audi Ichsani Christina Febiola Lubis Dianti Pratiwi Dila Qhoirul Nisa Elsya Martia Ivan Iduljana Lestari Mahardika Urbaningrum Nurma Dwi Rahmawati