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All Journal agriTECH Asian Journal of Agriculture SEMAR Bioscientist : Jurnal Ilmiah Biologi Berita Biologi
SOLICHATUN SOLICHATUN
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana Jl. PB. Sudirman Denpasar 80362 Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences

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Analisis Fitokimia dan Aktivitas Antioksidan Daun Kopi Robusta (Coffea canephora) Berdasarkan Perbedaan Ketinggian Tempat Tumbuh Nintowati, Purina; Solichatun, Solichatun; Suratman, Suratman
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13601

Abstract

Robusta Coffee Leaves are known to have high antioxidants that can stabilize free radical attacks. Based on the height of the place where Robusta Coffee grows, it has different characteristics, including producing different qualities and flavors from one another. This study aims to determine the content of phytochemical compounds and the antioxidant activity of Robusta coffee leaves based on differences in the height of the place where it grows. The research method used was sampling at several heights including 511 masl, 434 masl, 354 masl, 204 masl, 122 masl, and laboratory research for phytochemical and antioxidant tests. Data analysis used the ANOVA, DMRT, and Bivariate correlation tests to determine the correlation between height and antioxidant activity. The results showed that all five heights contained alkaloid and flavonoid compounds. The highest inhibition of antioxidant activity was found at an altitude of 511 masl with a percentage of 61.89% ±8.08b, while the lowest inhibition of antioxidant activity was found at an altitude of 122 masl with a percentage of 31.92% ±7.38a. Antioxidant activity at all five altitudes is classified as a very strong antioxidant with an IC50 value <50. Altitude has a significant effect (P<0.05) on antioxidant inhibition. Altitude has a positive correlation with antioxidant activity.
The Effect of Seed Priming Using Giberellic Acid (GA3) on Growth of Groundnut (Arachis hypogaea L.) and Lurik Groundnut (Arachis hypogaea L. var. Lurik) Tamonob, Arshinto; Solichatun, Solichatun; Suratman, Suratman
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13774

Abstract

Seed priming is a technique for treating seeds before germination to improve the quality of the plants produced. Seed priming can be done by soaking the seeds in various solutions, including hormone solutions. Groundnut (Arachis hypogaea L.) is the second most important commodity in Indonesia for both common and striated groundnut. This research aims to determine the effect of seed priming using the hormone gibberellic acid on the vegetative growth of peanuts and striated peanuts. This research used a completely randomized design with a factorial pattern; the first factor is variation in the type of peanut; The second factor is the variation in gibberellic acid concentration for seed priming, namely 0 ppm (only distilled water), 100 ppm, 200 ppm, 300 ppm and 400 ppm and without priming. Each treatment was carried out in 3 repetitions. The variables observed were plant wet weight, plant dry weight, chlorophyll content, number of leaves, shoot:root ratio. Quantitative data were analyzed using Analysis of Variance (ANOVA) and DMRT with a confidence level of 95%. The results of the research showed that seed priming treatment using the gibberellic acid hormone had a significant effect on the wet weight and dry weight of the plants; shoot:root ratio, and chlorophyll content of peanut and striated peanut leaves. Seed priming treatment using the hormone gibberellic acid had no significant effect on the number of leaves of ordinary peanuts and striated peanuts.
Analisis Fitokimia dan Aktivitas Antioksidan Daun Kopi Robusta (Coffea canephora) Berdasarkan Perbedaan Ketinggian Tempat Tumbuh Nintowati, Purina; Solichatun, Solichatun; Suratman, Suratman
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13601

Abstract

Robusta Coffee Leaves are known to have high antioxidants that can stabilize free radical attacks. Based on the height of the place where Robusta Coffee grows, it has different characteristics, including producing different qualities and flavors from one another. This study aims to determine the content of phytochemical compounds and the antioxidant activity of Robusta coffee leaves based on differences in the height of the place where it grows. The research method used was sampling at several heights including 511 masl, 434 masl, 354 masl, 204 masl, 122 masl, and laboratory research for phytochemical and antioxidant tests. Data analysis used the ANOVA, DMRT, and Bivariate correlation tests to determine the correlation between height and antioxidant activity. The results showed that all five heights contained alkaloid and flavonoid compounds. The highest inhibition of antioxidant activity was found at an altitude of 511 masl with a percentage of 61.89% ±8.08b, while the lowest inhibition of antioxidant activity was found at an altitude of 122 masl with a percentage of 31.92% ±7.38a. Antioxidant activity at all five altitudes is classified as a very strong antioxidant with an IC50 value <50. Altitude has a significant effect (P<0.05) on antioxidant inhibition. Altitude has a positive correlation with antioxidant activity.
The Effect of Seed Priming Using Giberellic Acid (GA3) on Growth of Groundnut (Arachis hypogaea L.) and Lurik Groundnut (Arachis hypogaea L. var. Lurik) Tamonob, Arshinto; Solichatun, Solichatun; Suratman, Suratman
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13774

Abstract

Seed priming is a technique for treating seeds before germination to improve the quality of the plants produced. Seed priming can be done by soaking the seeds in various solutions, including hormone solutions. Groundnut (Arachis hypogaea L.) is the second most important commodity in Indonesia for both common and striated groundnut. This research aims to determine the effect of seed priming using the hormone gibberellic acid on the vegetative growth of peanuts and striated peanuts. This research used a completely randomized design with a factorial pattern; the first factor is variation in the type of peanut; The second factor is the variation in gibberellic acid concentration for seed priming, namely 0 ppm (only distilled water), 100 ppm, 200 ppm, 300 ppm and 400 ppm and without priming. Each treatment was carried out in 3 repetitions. The variables observed were plant wet weight, plant dry weight, chlorophyll content, number of leaves, shoot:root ratio. Quantitative data were analyzed using Analysis of Variance (ANOVA) and DMRT with a confidence level of 95%. The results of the research showed that seed priming treatment using the gibberellic acid hormone had a significant effect on the wet weight and dry weight of the plants; shoot:root ratio, and chlorophyll content of peanut and striated peanut leaves. Seed priming treatment using the hormone gibberellic acid had no significant effect on the number of leaves of ordinary peanuts and striated peanuts.
Karakterisasi Morfologi dan Biokimiawi Berbagai Kultivar dan Aksesi Jeruk Pamelo (Citrus maxima (burm.) merr.) di Kabupaten Magetan Istiqomah, Intan Lailatul; Suratman, Suratman; Solichatun, Solichatun
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14984

Abstract

This study examines the morphological characteristics, sugar content, and antioxidant activity of various cultivars and accessions of Pomelo Orange in Magetan Regency. Samples were taken using a purposive sampling method. Morphological characters were observed based on IPGRI characterization, while antioxidant activity analysis was carried out using a UV-Vis spectrophotometer. Morphological character data were analyzed descriptively and antioxidant activity was analyzed by One Way Anova method and followed by DMRT (Duncan's Multiple Range Test) test. The results showed that (1) the highest inhibition percentage was found in the Srinyonya cultivar in Takeran, which was 76.15%; (2) Overall, Pomelo Orange cultivars have antioxidant activity with IC50 values ranging from 5.78–27.17 ppm, indicating strong antioxidant activity; (3) The sugar content in various cultivars ranges from 8–10 °Brix, with the highest levels found in the Javanese cultivar in Sukomoro, which was 10.7 °Brix.
Physicochemical Characteristics of Potato (Solanum tuberosum L.) after Chitosan Coating and Storage at Various Temperature Mudyantini, Widya; Pramesthi, Vionita Dyah; Suranto, Suranto; Solichatun, Solichatun; Pitoyo, Ari; Suratman, Suratman; Purnama, Purin Candra
agriTECH Vol 45, No 1 (2025)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.87199

Abstract

Potato (Solanum tuberosum L.) plant is widely favored due to its versatility in various processed products, but perishable. This study aimed to determine the effect of chitosan coating and storage at various temperatures on the physiological and chemical characteristics of potato tubers. This research used 2 variables, namely the concentration of chitosan (0, 1, 1.5 and 2%) and storage temperatures of 0, 10 and 25°C. The parameters observed included tuber skin color, weight loss, hardness, the thickness of the chitosan layer and tissue penetration, oxygen levels, water content, starch degradation, reduced sugar content, and sprouting amount. Furthermore, data were analyzed using the Analysis of Variance (ANOVA) test. The results showed the effect of chitosan coating and storage temperature on weight loss, color, oxygen level, hardness, water content, starch degradation, and reduced sugar content in potato tubers during storage. The most effective treatment was 1.5% chitosan coating combined with a storage temperature of 10°C.
ANALISIS KANDUNGAN KLOROFIL PADA DAUN DAN KANTONG Nepenthes gracilis [Analysis of Chlorophyll in Lamina and Pitcher of Nepenthes gracilis] Albar, Rahmat; Novitasari, Yuliana Diyah; Berlinda Paradisa, Yashanti; M, Des; Hilda Putri, Dwi; Chatri, Moralita Chatri; Solichatun, Solichatun; Wahyuni, Wahyuni
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.966

Abstract

Pitcher plants are plant species that have unique shapes. Nepenthes spp. in the feature has become an important issue about his developments in research on carnivorous plants and rare plants. Nepenthes spp. has been classified as a protected plant due to excessive exploitation, which can damage the place of origin. The research in this article focuses on analyzing the content of chlorophyll a, b, and carotenoid in leaves and pitchers of Nepenthes gracilis. Leaves and pitchers (fresh and dried) were extracted in 3 types of solvents (acetone, methanol, and DMSO). The samples were then analyzed with a UV-VIS spectrophotometer. The results showed that the leaf and pitcher samples had quite different results. The leaf sample showed higher results than the pitcher sample, which had a chlorophyll a (3.434a), chlorophyll b (1.311a), carotenoids (0.918a), and total chlorophyll (4.745a) content. The pitcher sample had a content of chlorophyll a (1.463b), b (0.642b), carotenoids (0.445b), and total chlorophyll (2.105b). The treatment of samples given various solvents such as DMSO, Acetone, and Ethanol showed insignificant results on the content of chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll. The conclusion is that all solvents can be used to analyze pigment content. Keywords: Carnivorous plant, Nepenthes gracilis, Pigment Content, Extracted solvents
Effects of TiO₂ nano-priming and field capacity levels on germination and growth of cayenne pepper (Capsicum frutescens) RAHMAWATI, NUR ANINDYA; SOLICHATUN, SOLICHATUN; PITOYO, ARI
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100112

Abstract

Abstract. Rahmawati NA, Solichatun, Pitoyo A. 2026. Effects of TiO₂ nano-priming and field capacity levels on germination and growth of cayenne pepper (Capsicum frutescens). Asian J Agric 10 (1): g100112. https://doi.org/10.13057/asianjagric/g100112. Capsicum frutescens is a vital crop in Indonesia but suffers from seasonal yield instability due to water stress. This study explored nano-priming with titanium dioxide nanoparticles (TiO₂NPs) to improve seedling resilience. The experiment was conducted in two stages. In the germination stage, four TiO₂NP concentrations (0%, 2%, 4%, and 8%) were tested on seed germination using a Completely Randomized Design (CRD). In the growth stage, seedlings were subjected to a 4 × 3 factorial CRD combining the same TiO₂NP concentrations with three levels of water availability (100%, 75%, and 50% field capacity), with three replicated. Data were analyzed using two-way ANOVA and Duncan’s Multiple Range Test (p < 0.05). During germination, nano-priming with 4% TiO₂NP accelareted germination rate (5.3 days) and enhanced sprout length (6.68 cm), whereas the control exhibited slower germination (9.03 days) and shorter sprouts (2.60 cm). In the subsequent growth phase, TiO₂NPs significantly influenced seedling height, shoot-to-root ratio, and proline content. The tallest seedling was observed at 8% TiO₂NP, particularly under 75% field capacity. In contrast, optimal shoot-to-root ratio and elevated proline accumulation were associated with 2% TiO₂NP under 100% and 75% field capacity, respectively. These finding demonstrated that TiO2NP nano-priming exerts stage-dependent effects, with distinct concentrations optimizing germination performance and drought-related physiological responses during early seedling growth, highlighting its potential as a scalable approach for improving C. frutescens cultivation under water-limited conditions.
The effect of brassinosteroid on cucumber (Cucumis sativus) growth under varying water availability NURROHMAH, AULIA; SOLICHATUN, SOLICHATUN; PITOYO, ARI
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100144

Abstract

Abstract. Nurrohmah A, Solichatun, Pitoyo A. 2026. The effect of brassinosteroid on cucumber (Cucumis sativus) growth under varying water availability. Asian J Agric 10 (1): g100144. https://doi.org/10.13057/asianjagric/g100144. Cucumber (Cucumis sativus) is a horticultural crop belonging the family Cucurbitaceae and is widely consumed in Indonesia. Cucumber production in the country tends to fluctuate due to extreme climate conditions, which cause variations in water availability and consequently disrupt plant growth. One approach to enhance cucumber tolerance to fluctuating water conditions is the application of brassinosteroid. This study aimed to evaluate the effects and determine the most effective concentration of brassinosteroid on cucumber growth under different level of water availability. A Completely Randomized Design (CRD) was employed with two factors and four replications. The first factor was field capacity (100%, 60%, and 40%), and the second was brassinosteroid (0, 0.5, 1, and 2 ppm). Cucumbers were grown for 40 days after transplanting and brassinosteroid was applied weekly as a foliar spray to the leaf surface. Data were analyzed using two-way Analysis of Variance (ANOVA) and followed by one-way unstucked ANOVA at a significance level of P < 0.05. Then tested by Duncan’s Multiple Range Test (DMRT) at a 95% confidence level. The Correlation analysis used Spearman's rank correlation coefficient. The results showed that brassinosteroid significantly improved cucumber growth parameters under different water availabilities. A brassinosteroid concentration of 0.5 ppm was most effective in improving vegetative growth parameters and plant biomass at 60% field capacity, while also increasing chlorophyll content under well-watered conditions (100% field capacity). In contrast, a higher concentration of 2 ppm promoted proline accumulation at 40% field capacity. The research suggest that lower brassinosteroid promote cucumber growth under moderate, whereas higher concentrations facilitate osmotic adjustment under severe drought conditions.
Pemetaan Plasma Nutfah dan Kualitas Ekspor Buah Vanili pada Koperasi Petani Vanili Karavan Donohudan Boyolali Mudyantini, Widya; Suranto, Suranto; Solichatun, Solichatun; Pitoyo, Ari; Suratman, Suratman; Purnama, Purin Candra
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 14, No 1 (2025): Mei
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v14i1.94479

Abstract

Membership of the Indonesian Vanilla Spice producer cooperative "KARAVAN" with a Secretariat office in Donohudan-Surakarta, consisting of 18 districts/cities including Semarang, Ungaran, Kudus, Pati, Grobogan, Blora, Salatiga, Temanggung, Magelang, Kendal, Pekalongan, Karanganyar, Wonogiri , Tegal, Surakarta, Purwokerto, Banyumas, Purworejo, Kulonprogo (DIY). The number of members of the 126 farmer cooperative is around 1300 farmers. The production capacity of assisted vanilla farmers for green reaches 7-8.5 tons of green with a distribution on the Muria slope of around 1-1.5 tons. Salatiga, Ambarawa, Temanggung 2 tons. Wonosobo, Pekalongan, Kendal to Tegal around 3-4 tons, Magelang Purworejo, Banyumas around 2.5-3.5 tons per year. Some potential buyer countries include Switzerland, Germany, Malaysia, the Netherlands and Taiwan. One of the requirements for export is proof of vanillin content testing from a laboratory that has been accredited by KAN, including the UNS Integrated Laboratory UPT. Funding assistance for vanillin level testing is very necessary for farmers. This service helps with the costs of testing vanillin levels at different locations and finding optimal post-harvest handling standards for all members so that the results of vanillin levels can be uniform. Activities that have been carried out include holding an FGD between Karavan farmer cooperative partners and the UNS Integrated Laboratory UPT, BSN (National Standardization Agency) and the Plant Biomaterials Research Group on Wednesday, June 12 2024. This activity aims to make the vanilla farmer cooperative a BSN foster partner in assisting with SNI registration. Also making service partners the main clients in testing vanillin levels at the UNS Integrated Laboratory UPT. Various issues regarding germplasm, cultivation and post-harvest are assisted by the Plant Biomaterials Research Group. This service also involved taking part in an exhibition for the 48th Anniversary in front of the Rectorate on Friday – Saturday 21-22 June 2024. To train and apply the technology for drying vanilla fruit in an oven, research was carried out based on existing journals on vanilla fruit. The best treatment for drying and heating treatment is a water temperature of 55 oC and an oven of 40 oC ( on September 19). The vanillin content test was carried out and the levels were measured in 9 samples from 7 regions. From Tegal (Guci area) the vanillin levels were 1.70%, 1.78% and 1.73% with a standard deviation of 0.04%, the three samples were not significantly different. From Kudus (Mount Muria) 1.86%, Grobogan Sukolilo (North Gendeng) 1.54%, Lumajang (Bromo) 1.20%, Ambarawa 1.46%, Pati 1.59%, Magelang (Gunung Kelir-Ngablak) 1, 52%. The highest levels were from Kudus, followed by Tegal, Pati, Grobogan, Magelang, Ambarawa, and the lowest was Lumajang. Kata kunci :  Vanillin levels, germplasm, exports, Karavan vanilla farmers' cooperative 
Co-Authors Albar, Rahmat Berlinda Paradisa, Yashanti Chatri, Moralita Chatri Des M Dwi Hilda Putri Istiqomah, Intan Lailatul Nintowati, Purina Novitasari, Yuliana Diyah Nurrohmah, Aulia Pitoyo, Ari Pramesthi, Vionita Dyah Purnama, Purin Candra Rahmawati, Nur Anindya Suranto Suranto Suratman Suratman Tamonob, Arshinto wahyuni wahyuni WIDYA MUDYANTINI