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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI Kebijakan: Jurnal Ilmu Administrasi Jurnal DIALEKTIKA : Jurnal Ilmu Sosial Journal of Community Research & Engagement (JCRE)
Sri Sulandari
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary

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Journal : Jurnal Veteriner

 1 
Investigasi Asal Usul Ayam Indonesia Menggunakan Sekuens Hypervariable-1 D-loop DNA Mitokondria Moch Syamsul Arifin Zein; Sri Sulandari
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Chicken taxonomy and pedigree investigation are important parts to understand the domesticationprocess occurred on chicken in Indonesia. The Indonesian native chickens have very different clade with thechickens from other Asian countries and others. This study was conducted to construct phylogeny junglefowls (red and green jungle fowls) and Indonesian native chickens, in order to know their relationship.Among the available DNA marker, the region D-loop on DNA mitochondria is the most effective markerused in the investigation. Mitochondrial DNA D-loop (hypervariable-1 segment) was PCR amplified andsubsequently sequenced for a total 33 individuals of green jungle fowls (Gallus varius), 9 individuals of redjungle fowls (Gallus g. gallus) from Indonesia, and 30 individuals of Indonesian native chickens (Lombok,Cemani, Kedu, Kedu Putih, Nunukan, Kate, Pelung, Gaok, Merawang, dan Sentul). Seventy two (72)sequences were used for analysis. Seven (7) published reference D-loop sequences of genus Gallus fromGenBank were also included in the analysis: Gallus varius (GenBank accession number D64163 danD82912), Gallus gallus (GenBank accession number AB098668), G. gallus spadiceus (GenBank accessionnumber AB007721), Gallus gallus bankiva (GenBank accession number AB007718), Gallus lafayetti(GenBank accession number D66893), and Gallus sonneratii (GenBank accession number D66892). Phylogenyanalysis indicates that Indonesia jungle fowls can be grouped into two clades (clades of red jungle fowls/Indonesia native chicken and green jungle fowls). Monophyletic phylogeny trees of jungle fowls from Indonesiawere shown in this study for pedigree investigation of domesticated chickens.
Keragaman Fenotipik dan Pendugaan Jarak Genetik pada Ayam Lokal dan Ayam Broiler Menggunakan Analisis Morfologi (PHENOTYPIC VARIATION AND ESTIMATION GENETIC DISTANCE BETWEEN LOCAL CHICKEN AND BROILER CHICKEN USING MORPHOLOGICAL ANALYSIS) Harini Nurcahya Mariandayani; Dedy Duryadi Solihin; Sri Sulandari; Cece Sumantri
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This aim of the research was to study the morphological characteristic and estimating genetic distancebetween local chicken and broiler chicken with discriminant and canonical analysis. This research washeld in Faculty of Animal Husbandry, Bogor Agricultural University, using 25 sentul chickens,  25  kampongchickens, 25 kedu chickens , 25  pelung chickens and 25 broiler chickens. The variable as the length ofshank, beak length, back length, chest depth and chest width were measured in this study. The collecteddata were analyzed by using SAS  and SPSS package program. Kampung chickens were mixed with sentulchickens (17.60 %) and kedu chickens (17,70 %). Kedu, kampong,  and  sentul chickens have a relativelyclose genetic distance   compared the genetic distance to pelung chickens with the kampung, sentul, andkedu chickens. Fenogram tree show that there were three separate groups of chickens at the age of eightweeks i.e. : (1) pelung chickens (2), kedu, kampong, and sentul chickens, (3) broiler chickens.  Fenogram treealso shows two separate groups : (1) pelung chickens (2) kedu, kampong, and Sentul chickens (at the age of28 weeks chicken).  The crossbreed between kedu and sentul chickens, also have a relatively close geneticdistance. The phenotypic size of  chickens giving a strong influence on the distinction variable of chickengroups were body length and chest circumference.
Genetic Polymorphisms of The Chicken Antiviral Mx Gene in A Variety of Indonesian Indigenous Chicken Breeds Sri Sulandari; Moch Syamsul Arifin Zein; Dwi Astuti; Tike Sartika
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

It has previously been demonstrated that a G/A Single Nucleotide Polymorphism (SNP) at nucleotideposition 1,892 of coding sequence of chicken Mx gene confers susceptibility/resistance to avian viral diseases.The aim of this study was to assess the geographical distribution of G/A alleles in relation to differentgenetic backgrounds of a wide range of chicken populations. Using Polymerase Chain Reaction- RestrictionFragment Length Polymorphism (PCR-RFLP) methods, 492 samples from 15 breeds of indigenous chickenpopulations from Java, Sumatera, Kalimantan and Sulawesi islands were genotyped. Allele and genotypefrequencies of each population were calculated. Deviations from Hardy-Weinberg equilibrium were testedand inbreeding coefficient FIS estimated. Overall, the susceptible allele G had a frequency of 37.27% whilethe resistant allele A had a corresponding frequency of 62.73%. No clear relation of the geographicaldistribution of the G/A alleles to genetic backgrounds was found. The distribution of this SNP acrosspopulations seems to be affected by genetic drift rather than selection.
Sidik Jari DNA dan Fenotipe pada Populasi Kambing Gembrong dengan Status Kritis di Karangasem, Bali (DNA FINGERPRINT AND PHENOTYPE ON THE CRITICAL POPULATION STATUS OF GEMBRONG GOAT IN KARANGASEM REGENCY, BALI) Moch Syamsul Arifin Zein; Sri Sulandari
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Gembrong goat is animal genetic resources which endemic in Bali island and only exists in Karangasemregency. The current population status of the gembrong goat is critical. Therefore, the population developmentof in-situ or ex-situ needs to be conducted. Close relative mating tend to occur in conditions such as in asmall population, critical status or limited distribution. Low degree of genetic variability is often found insuch conditions. Mating arrangements based on DNA data of fingerprint was developed in this study toavoid mating between close relatives (inbreeding). Pedigree detection was analyzed using fifteen (15)microsatellite markers as recommended by ISAP/FAO. The results showed that polymorphic alleles foundin the microsatellite markers of SRCRSP3, ILSTS005, MCM527 (4 alleles), followed BM1818 (3 alleles),and ILSTS029, BMS1494, MAF035, OARFCB20, OARE54, MAF70, ILSTS11, ETH10 (2 alleles).Monomorphic allele (1 allele) was also found in microsatellite markers of SPS113, CSRD247, INRA0132.DNA fingerprint of the gembrong goat population was created by genetic distance between individuals,and indicated six clades / haplogroups. It is suggested from this study that mating arrangements betweendifferent clades should be applied to increase genetic diversity. Description of morphologies such as haircolor and body size, were used as basic considerations in determining the authenticity of gembrong goat.Among the population of gembrong goat in this study, hair color of the goat showed white (78.95%), amixture of light brown and white (15.79%), and a mixture of brown and black (5.26%). Gembrong goat bodysize in this study was still in the range of the previous studies. The analysis results of DNA fingerprintsand phenotypes can be used as a basis for rescue and development of gembrong goats, in an attempt toform a large gene pool with high viability for the conservation, development and sustainable uses.
Co-Authors Agus Kundarmasno Alwin Marakarmah Cece Sumantri Cokorda Putra Indrayana Dede, I Kadek Dede Junaedy Dwi ASTUTI Eka, I Gede Agus Eka Pradnyana Gede Wirata Harini Nurcahya Mariandayani i Wayan Astawa JUMANTO HARJOSUDARMO M S A Zein M. Syamsul Arifin Zein Moch Syamsul Arifin Zein Moch Syamsul Arifin Zein Ni Luh Putu Ening Permini Ni Nyoman Reseani Nyoman Suargita Putu Deva Pradnyana Mardiana Putera RUSMILAH SUSENO Satrio Wijamukti Soemartono Sosromarsono SRI HENDRASTUTI HIDAYAT Sri Paryanti TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TIKE SARTIKA Tri Hayatining Pamungkas