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All Journal HAYATI Journal of Biosciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI Kebijakan: Jurnal Ilmu Administrasi Jurnal DIALEKTIKA : Jurnal Ilmu Sosial Journal of Community Research & Engagement (JCRE)

Sri Sulandari

Unknown Affiliation

Author-ID : 385832

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary

Published : 11 Documents Claim Missing Document

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Molecular Characterization of Indonesian Indigenous Chickens based on Mitochondrial DNA Displacement (D)-loop Sequences SRI SULANDARI; MOCH SYAMSUL ARIFIN ZEIN; TIKE SARTIKA
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

The Mitochondrial DNA (mtDNA) displacement (D)-loop sequences were used to study the genetic diversity and relationship of Indonesian indigenous chickens. A total of 483 individuals belonging to 15 population breeds and 43 individuals belonging to 6 populations of jungle fowl (2 populations of Gallus gallus and 4 populations of Gallus varius) were sampled. The hypervariable I (HVI) segment of the D-loop was PCR amplified and subsequently sequenced. The sequences of the first 397 nucleotides were used for analysis. Sixty nine haplotypes were identified from 54 polymorphic sites with polymorphism between nucleotides 167 and 397 contributing to 94.5% of the sequence variation. Phylogenetic analysis indicates that Indonesian indigenous chickens can be grouped into five distinct clades (clade I, II, IIIc, IIId, and IV) of the previously identified seven clades (clade I, II, IIIa, IIIb, IIIc, IIId, and IV) in Asian indigenous chickens. Fifty haplotypes belong to clade II, seven haplotypes are in clade IV, six are in clade IIId, three are in clade I and one haploype is in clade IIIc. There was no breed-specific clade. Analysis of Molecular Variance (AMOVA) based on partial D-loop sequences of Indonesian chicken indicates that 67.85% of the total sequence variation between haplotypes was present within the population and 32.15% between populations. One of the haplotypes (represented by PLC4) was shared by all populations, suggesting that these populations may share the same maternal ancestor. These results show a high mitochondrial D-loop diversity and indicate multiple maternal origins for Indonesian indigenous chickens. Key words: Indonesian indigenous chicken, mitochondrial DNA, D-loop, haplotype, phylogenetic analysis and clade

Detection and Host Range Study of Virus Associated with Pepper Yellow Leaf Curl Disease SRI SULANDARI; RUSMILAH SUSENO; SRI HENDRASTUTI HIDAYAT; JUMANTO HARJOSUDARMO; SOEMARTONO SOSROMARSONO
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

High incidence of Pepper yellow leaf curl virus (PepYLCV) was observed in Indonesia since early 2000. Disease incidence in Yogyakarta, Central and West Java reached 100% on Capsicum frutescens, but only 10-35% on C. annuum. As an exception, the disease incidence on C. annuum cv. TM 999 was in the range of 70-100%. The causal agent of the disease, PepYLCV, was detected by polymerase chain reaction. Viral specific DNA fragment of the size ~1600 bp and ~550 bp was amplified from infected plants using two pairs of geminivirus universal primers pAL1v1978/pAL1c715, and pAv494/pAc1048, respectively. The PepYLCV has an intermediate host range including plants belonging to the family of Solanaceae, Leguminosae, and Compositae. The species belonging to the families of Cucurbitaceae, Malvaceae, Chenopodiaceae, and Amaranthaceae were resistant to the virus. Physalis floridana, is very prospective as a propagation host for the geminivirus infecting pepper. Nicotiana spp., cucumber, watermelon, cotton, and Sida sp. could be used as a differential host. Besides, Capsicum frutescens cv. Cakra, tomato, N. benthamiana, N. glutinosa, and Ageratum conyzoides could be used as indicator plants for the geminivirus infecting pepper. Key words: Pepper yellow leaf curl virus, disease incidence, host range

Analysis of genetic relationship among Indonesian native chicken breeds based on 335 D-loop sequences Sri Sulandari; M. Syamsul Arifin Zein; Tike Sartika
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 4 (2008): DECEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The Mitochondrial DNA (mtDNA) D-loop segment was PCR amplified and subsequently sequenced for a total of 335 individuals from Indonesian native chicken. The individuals were drawn from sixteen populations of native chicken and three individuals of green jungle fowls (Gallus varius). Indonesian native chicken populations were: Pelung Sembawa, PL (n = 18), Pelung Cianjur, PLC (n = 29) and Arab Silver, ARS (n=30), Cemani, CM (n = 32), Gaok, GA (n = 7), Kedu Hitam, KDH (n = 11), Wareng, T & TW (n = 10), Cemani, CMP (n = 2), Kedu, KD (n=26), Kedu Putih, KDP (n = 15), Sentul Jatiwangi, STJ (n = 27), Ayam Kate, KT (n = 29), Ayam Sentul, STC (n = 15), Arab Golden, ARG (n = 26), Ayam Merawang, MR (n = 28), Kedu Putih Jatiwangi, KDPJ (n=6) and Kapas, KPS (n = 21). Green jungle fowls were: two individuals from Flores island (FL5 and FL57) and one individual (BD42) from Sumbawa island. The sequences of the first 530 nucleotides were used for analysis. Eighty two haplotypes were identified from 78 polymorphic sites for the 335 individuals. Seventy nine haplotypes were identified in native chicken from 57 polymorphic sites while three were of jungle fowls. Phylogenetic analysis indicates that Indonesian native chicken can be grouped into five clades (Clade I, II, IIIc, IIId and IV) of the previously identified seven clades (Clade I, II, IIIa, IIIb, IIIc, IIId and IV) in Asian domestic chicken. Haplotypes CM10 and CM32 fall to a different category while STC12 is also on its own. Interestingly STC12 clusters together with Gallus gallus gallus (GenBank accession No. SULANDARI et al. Analysis of genetic relationship among Indonesian native chicken breeds based on 335 D-loop sequences 296 AB007720). When CM10 (same as CM14), CM32 and STC12 were removed, 77 haplotypes of domestic chicken were identified from 53 polymorphic sites. All the green jungle fowls are clustered to one clade of their own. The clades of domestic chicken are: Clade I which has three haplotypes, Clade II has 52 haplotypes, Clade IIIc has one haplotype (represented by ARS30), Clade IIId has nine haplotypes while Clade IV has eleven haplotypes. The phylogenetic relationship between chicken populations has no link to the geographic locations. Analysis of molecular variance showed that the genetic variation within populations was 67.42% while 32.58% accounted for the genetic differentiation between populations. Key Words: Native Chiken, Green Jungle Fowls, D-Loop DNA Mitochondria, HV-1, Clade, Haplotype, Phylogenetic, Genetic Variation

Genetic diversity of Lombok chickens based on D-loop mitochondrial DNA sequences M. Syamsul Arifin Zein; Sri Sulandari
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 4 (2008): DECEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Mitochondrial DNA (mtDNA) displacement (D)-loop sequences were used to study the genetic diversity and relationship of Lombok chickens. A total of 45 individuals were sampled. The D-loop segment was PCR amplified and subsequently sequenced. The sequences of the 785 nucleotides were used for analysis. Twelve haplotypes were identified from 25 polymorphic sites with polymorphism between nucleotides 200 and 400 contributing to 80% of the variation. Fu’s Fs value was - 8.768 (all samples, P = 0), indicating high genetic diversity and population expansion, a conclusion supported by a neighbor– joining analysis of the haplotypes. Nucleotides diversity of the Lombok chicken were 0.00221 and haplotype diversity were 0.654 + 0.08. The dominant haplotype found among the Lombok chickens was haplotype B (62%) and genetic distances value ranged from 0.001 to 0.017. Key Words: Mtdna, D-Loop, Genetic Diversity, Haplotype, Lombok Chicken

Nunukan Chicken: Genetic Characteristics, Phenotype and Utilization Tike Sartika; Sri Sulandari; M S A Zein; Sri Paryanti
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 4 (2006): DECEMBER 2006
Publisher : Indonesian Center for Animal Research and Development

Nunukan chicken is a local chicken from East Kalimantan which spreads out in Tarakan and Nunukan Islands . The chicken has a specific buff color and Columbian type feather and also has very late feathering (VLF) trait . The Nunukan cocks and hens have no wing and tail primary feather; the tail feathers are short and fragile . The VLF trait is known to have association with a K gene on the Z chromosome. The chicken is efficient in protein metabolism . Sulfur amino acids (cystine and methionine) that needed for feather growth, could be utilized for meat and egg production . The egg production of Nunukan chicken was better than the Kampung chicken . The average of hen day, hen house and peak production of Nunukan chicken was 45 . 39.1 and 62%, respectively, while the Kampung chicken was 35 .9, 30 .9 and 48%, respectively . Based on genetic analysis, the external genotype characteristic of the Nunukan chicken is ii ce ss Idld pp. It means that the phenotype appearance of the Nunukan chicken was columbian and gold feathering type, yellow and white shank color and single comb type. This phenotype is similar to Merawang Chicken . The genetic introgression of the Nunukan chicken is affected by the Rhode Island Red with the genetic introgression value of 0.964 . Key words: Nunukan chicken, character, genetic; phenotype characteristics, utilization

Struktur Populasi Genetik Ayam Hutan Hijau Menggunakan Sekuen Hypervariable 1 D-Loop DNA Mitokondria M. Syamsul Arifin Zein; Sri Sulandari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i3.2573

Thirty-three individuals from four populations of green junglefowl (Gallus varius) were collected throughout Indonesia: 14 from Central Java, 4 from Eastern Java, 3 from Sumbawa island and 12 from Flores island. The mitochondrial DNA D-loop the samples were analysed for sequence diversity. Twenty-five haplotypes with 28 polymorphic sites were identified within the first hypervariable-1 fragment (397 bp) of the D-loop. Fu’s Fs value was -25.96 (all samples, P=0), indicating high genetic diversity and population expansion; a conclusion supported by a neighbor–joining analysis of the haplotypes. Though sample size per population varied between 3 and 14, the Fs values for the four populations, between -2.20 and -10.76, were all significant (P=0). Only one haplotype was shared between three populations (Central Java, Sumbawa island, and Flores island) by a total of seven individuals. Within populations, only three haplotypes were shared by three individuals. The remaining haplotypes were unique, indicating genetic differentiation between populations as confirmed by significant pairwise Fst values at P=0.05 in four out of the six population pairs (except two pairs of Central Java & Sumbawa island and Flores island & Sumbawa island). The diversity pattern observed at the mtDNA of the green junglefowl provides a baseline which may help to understand the recent population expansions of domesticated chickens from multiple centres of domestications. Our observations also suggest careful interpretation of the results of genetic characterization may be needed when applied to the management and conservation of species like the green junglefowl. As in other multiparous birds and mammals with a short reproductive interval, green junglefowl may have established distinct genetic entities in metapopulations across its geographic distribution.

COLLECTION OF MATERIAL DNA SAMPLES FROM BIRDS IN THE GUNUNG HALIMUN NATIONAL PARK (GHNP) FOR ESTABLISHING OF DNA BANK Sri Sulandari; Dwi Astuti; Agus Kundarmasno; Alwin Marakarmah; Satrio Wijamukti
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1080

This study was conducted to collect material DNA samples from birds in the Gunung Halimun National Park (GHNP) for establishing of DNA bank.Ciptarasa, Geger Hanjuang and Cikaniki village areas were chosen as sites for sample collections in the GHNP.In order to take the sample from birds, transect lines were established at each site where series of mist nets were operated.The captured birds, both live- and died-birds, were identified. For the living birds, blood and/or shed feathers were taken from the bird prior to releasing.In case of died birds, tissue and liver were collected. The collected samples were transported to genetic laboratory at Zoological Division,Researh Center for Biology (RCB)-LIPI (The Indonesian Institute of Science) and kept in 4°C. In this collection, a total of 411 collected samples were obtained, coming from 25 families and 79 species.The 19-endemic birds were found in GHNP.

ANALISIS KEBIJAKAN PENATAAN KAWASAN SEMPADAN JURANG DALAM MEWUJUDKAN GREEN INFRASTRUCTURE DI KABUPATEN BANGLI Dede, I Kadek Dede Junaedy; Ni Luh Putu Ening Permini; Sri Sulandari; Nyoman Suargita
Kebijakan : Jurnal Ilmu Administrasi Vol. 16 No. 02 (2025): Volume 16 No. 2 Juni 2025
Publisher : Program Magister Ilmu Administrasi dan Kebijakan Publik, Pascasarjana, Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/kebijakan.v16i02.19447

Pembangunan rumah makan, café atau restaurant pada kawasan sempadan jurang di Kintamani semakin marak terjadi, hal ini menjadi permasalahan yang belum dapat diatasi oleh pemerintah sampai saat ini. Pemerintah telah menetapkan Perda RTRW terbaru untuk mengatur penataan/pemanfaatan kawasan sempadan jurang, namun saat ini masih marak terjadi pelanggaran pembangunan di kawasan sempadan jurang.Tujuan dari penelitian ini untuk menlakukan analisis mendalam terhadap pelaksanaan kebijakan penataan kawasan sempadan jurang dalam mewujudkan green infrastruktur di Kabupaten Bangli serta untuk mengetahui faktor pendukung dan penghambat dari pelaksanaan kebijakan tersebut. Metode yang digunakan pada penelitian ini, yaitu metode kualitatif dengan pendekatan fenomenologi. Luaran yang ditargetkan pada penelitian ini, yaitu strategi dalam pelaksanaan kebijakan penataan kawaswan sempadan jurang dan pengembangan konsep green infrastruktur.

Effectiveness of Business License Renewal Services (NIB for MSMEs) Through the OSS System at the Investment and One-Stop Integrated Services Office of Denpasar City Ni Nyoman Reseani; Sri Sulandari
Jurnal Dialektika: Jurnal Ilmu Sosial Vol. 23 No. 2 (2025): Jurnal Dialektika: Jurnal Ilmu sosial
Publisher : Pengurus Pusat Perkumpulan Ilmuwan Administrasi Negara Indonesia (PIANI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63309/dialektika.v23i2.641

Abstract This research aims to analyze the effectiveness of business license extension services through the Online Single Submission (OSS) system at the Investment and Integrated One-Stop Service Office (DPMPTSP) of Denpasar City. OSS, as part of the digital transformation of public services, is designed to improve the efficiency, transparency, and accessibility of licensing services, particularly for Micro, Small, and Medium Enterprises (MSMEs). The research results indicate that administratively, the implementation of OSS has had a significant positive impact on the increase in the issuance of Micro and Small Business Licenses (IUMK), from 888 licenses in 2018 to 2,671 licenses in 2022. However, the effectiveness of the system is not yet optimal, marked by a realization of license achievements only reaching 89.03% of the target, and the continued high dependence of the community on manual assistance due to low digital literacyAnalysis using e-Government theory and the Design-Reality Gap from Heeks (2006) shows a gap between system design and the real capabilities of users. Identified barriers include limited access to technology, low technical understanding, and a lack of consistent support and socialization. Additionally, inadequate institutional capacity, minimal cross-sector integration, and still passive communication strategies also affect service effectiveness. Therefore, strengthening human resources, training in digital literacy, and developing an inclusive and adaptive service ecosystem are needed for OSS to truly function as an effective and equitable digital public service instrument. Keywords: Effectiveness, OSS, business licensing, MSMEs, e-government, Denpasar

The Effectiveness of Motor Vehicle E-SAMSAT Services (A Research Study at the Badung Regency SAMSAT Office) Eka, I Gede Agus Eka Pradnyana; I Wayan Astawa; Sri Sulandari
Jurnal Dialektika: Jurnal Ilmu Sosial Vol. 23 No. 2 (2025): Jurnal Dialektika: Jurnal Ilmu sosial
Publisher : Pengurus Pusat Perkumpulan Ilmuwan Administrasi Negara Indonesia (PIANI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63309/dialektika.v23i2.684

Abstract Taxes are the main source of state revenue and play a crucial role in funding development and providing public services. In Indonesia, one significant type of tax that contributes to regional revenue is the Motor Vehicle Tax (PKB). The government continuously strives to improve taxpayer compliance through technology-based public service innovations, one of which is the E-Samsat service. This service aims to simplify the process of paying motor vehicle taxes online in order to increase efficiency and public participation. This study employs a qualitative research method with a descriptive approach, aiming to gain a deeper understanding of social phenomena occurring in natural settings. As such, the study presents new insights, particularly by identifying several key aspects related to the Effectiveness of E-Samsat Services for Motor Vehicles in Badung Regency, including (1) Goal Achievement, (2) Adaptation, and (3) Integrity. The objective of this research is to analyze the role of the E-Samsat service in improving motor vehicle taxpayer compliance in Badung Regency, as well as to identify the challenges faced by the public in using this service. The results of the analysis are expected to provide strategic recommendations for local governments to optimize the use of technology in order to improve tax compliance and achieve regional revenue targets. Factors influencing the effectiveness of the E-Samsat service in the Badung Regency Samsat Office include the public's willingness to change old habits into new ones. These habits refer to the continued use of third-party agents (biro samsat) to handle vehicle tax payments, and the tendency of many people to still come directly to the Samsat office to pay their motor vehicle taxes. Keywords: Motor Vehicle Tax, E-Samsat, Taxpayer Compliance, Public Service, Badung Regency

Co-Authors Agus Kundarmasno Alwin Marakarmah Cece Sumantri Cokorda Putra Indrayana Dede, I Kadek Dede Junaedy Dwi ASTUTI Eka, I Gede Agus Eka Pradnyana Gede Wirata Harini Nurcahya Mariandayani i Wayan Astawa JUMANTO HARJOSUDARMO M S A Zein M. Syamsul Arifin Zein Moch Syamsul Arifin Zein Moch Syamsul Arifin Zein Ni Luh Putu Ening Permini Ni Nyoman Reseani Nyoman Suargita Putu Deva Pradnyana Mardiana Putera RUSMILAH SUSENO Satrio Wijamukti Soemartono Sosromarsono SRI HENDRASTUTI HIDAYAT Sri Paryanti TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TIKE SARTIKA Tri Hayatining Pamungkas

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