Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biologica Samudra
Sri Sumarni Sri Sumarni
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Neuroscience Veterinary

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Characterization of antigens of hookworm larvae (Ancylostoma spp.) Sri Sumarni, Sri Sumarni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 24, No 04 (1992)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.947 KB)

Abstract

Cutaneous larva migrans (CLM) is caused by animal hookworm larvae in man. The conventional diagnosis and treatment of CLM will injure the skin of the patients, and the results are not satisfying. Therefore it should be necessary to do the serological test to diagnose CLM.The antigens were prepared by extracting the hookworm larvae in CTAB detergent, and injected into Balb/C mice. Characterization of antigens were done, quantitatively (by spectrophotonieter) and qualitatively (by SDS PAGE electrophoresis).The results showed that the titre rate of hookworm larvae antigens was 145 ug/ml. The antigens consist of 4 protein fractions with molecular weight of 25 KD, 55 KD, 66 KD and above 66 KD. Those antigents apparently generated high immune response in immunized miceKey Words; Ancylostoma spp. - CTAB detergent - SDS PAGE electrophoresis - immunology - parasitology
Evaluation of in vitro culture media of adult Brugia malayi with glucose supp/ementetipn Sri Sumarni, Sri Sumarni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 03 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.498 KB)

Abstract

Background: The circulating antigen of filariasis malayi patient is the excretory/secretory (ES) protein. It was used as antigen in monoclonal antibody production. The ES protein can be obtained by in vitro culture of filarial worm Brugia malayi.Objective : The aims of this research was to evaluate in vitro culture media with glucose supplementation of adults B.malayi to obtain the ES protein maximally.Methods: Adult males and females B.malayi were cultivated in vitro in medium with glucose supplementation in different concentrations (1%, 3% and 5%). The culture media were changed daily and analysis of its protein were carried out quantitatively using Bradford method, and gel electrophoresis (SDS PAGE) for qualitative protein analysis. The cuticle of adults B.malayi were extracted by detergent cetyltrimethyl ammonium bromide (CTAB).Results: The effective culture medium was RPMI with 1% glucose supplementation to produce ES protein maximally, and longevity of worms could survive for 6 days. The quantitative protein concentration of female worms culture medium was 0.2-1.85 ug/ml, and the protein molecular weight of female worms cuticle was 26 kDa- 116 kDa. The protein concentration of male culture medium was 0.2-0.5 ug/ml, the molecular weight of adult male cuticle protein was 34 kDa- 56kDa. The peak concentration of protein in both cultivation was observed on the third day.Conclusion: The best medium for in vitro cultivation of adults B.malayi was RPMI with 1% glucose supplementation. The protein concentration of the female culture media was higher than male .Key words: glucose supplementaion - in vitro culture - filarial worms -Brugia malayi - ES protein
Evaluation of in vitro culture media of adult Brugia malayi with glucose supp/ementetipn Sri Sumarni Sri Sumarni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 03 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.498 KB)

Abstract

Background: The circulating antigen of filariasis malayi patient is the excretory/secretory (ES) protein. It was used as antigen in monoclonal antibody production. The ES protein can be obtained by in vitro culture of filarial worm Brugia malayi.Objective : The aims of this research was to evaluate in vitro culture media with glucose supplementation of adults B.malayi to obtain the ES protein maximally.Methods: Adult males and females B.malayi were cultivated in vitro in medium with glucose supplementation in different concentrations (1%, 3% and 5%). The culture media were changed daily and analysis of its protein were carried out quantitatively using Bradford method, and gel electrophoresis (SDS PAGE) for qualitative protein analysis. The cuticle of adults B.malayi were extracted by detergent cetyltrimethyl ammonium bromide (CTAB).Results: The effective culture medium was RPMI with 1% glucose supplementation to produce ES protein maximally, and longevity of worms could survive for 6 days. The quantitative protein concentration of female worms culture medium was 0.2-1.85 ug/ml, and the protein molecular weight of female worms cuticle was 26 kDa- 116 kDa. The protein concentration of male culture medium was 0.2-0.5 ug/ml, the molecular weight of adult male cuticle protein was 34 kDa- 56kDa. The peak concentration of protein in both cultivation was observed on the third day.Conclusion: The best medium for in vitro cultivation of adults B.malayi was RPMI with 1% glucose supplementation. The protein concentration of the female culture media was higher than male .Key words: glucose supplementaion - in vitro culture - filarial worms -Brugia malayi - ES protein
Characterization of antigens of hookworm larvae (Ancylostoma spp.) Sri Sumarni Sri Sumarni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 24, No 04 (1992)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.947 KB)

Abstract

Cutaneous larva migrans (CLM) is caused by animal hookworm larvae in man. The conventional diagnosis and treatment of CLM will injure the skin of the patients, and the results are not satisfying. Therefore it should be necessary to do the serological test to diagnose CLM.The antigens were prepared by extracting the hookworm larvae in CTAB detergent, and injected into Balb/C mice. Characterization of antigens were done, quantitatively (by spectrophotonieter) and qualitatively (by SDS PAGE electrophoresis).The results showed that the titre rate of hookworm larvae antigens was 145 ug/ml. The antigens consist of 4 protein fractions with molecular weight of 25 KD, 55 KD, 66 KD and above 66 KD. Those antigents apparently generated high immune response in immunized miceKey Words; Ancylostoma spp. - CTAB detergent - SDS PAGE electrophoresis - immunology - parasitology
Profil of Swamp Water Fish in Manter Village, Sintang Regency, West Borneo Rizkiyani, Ainun; Afriani, Rachmi; Nazarudin, Nazarudin; Sri Sumarni, Sri Sumarni
JURNAL BIOLOGICA SAMUDRA Vol 7 No 2 (2025): Biologica Samudra
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jbs.v7i2.13365

Abstract

This study aims to describe the profile of swamp fish resources based on community knowledge in Manter Village, Sintang Regency. The community’s understanding of the potential of local fish resources can be developed into village government policies to optimize the potential of local resources thereby supporting increased income and community welfare while promoting food self-sufficiency. This research employed interview and observation methods. Interviews were conducted with local community members to gather information on fish species found in swamp waters, while observations were carried out to determine the sampling points. Sampling points were determined using purposive sampling in four hamlets: Pandan Arum, Sidodadi, Purwosari, Simpang Manter. The results showed that there were 11 swamp fish species found in Manter Village, including Sepat Rawa (Trichogaster trichopterus), Sepat Mutiara (Trichopodus leeri), Cucut (Luciocephalus pulcer), Toman (Channa micropeltes), Gabus (Channa striata), Betok (Anabas tustudineus), Lele Lembat (Clarias nieuhifii), Seluang (Rasbora sp), Biawan (Helostoma temincki), Sili Batik (Mastacembulus armatus), dan Belut Rawa (Synbranchus bengalensis). The fish resources found in Manter Village are primarily utilized directly by the community for household consumption. These fish species can be further developed by the community through processed products to increase income and improve community welfare, ultimately contributing to achieving food-self sufficiency in Manter Village.
Co-Authors Afriani, Rachmi Nazarudin Rizkiyani, Ainun