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All Journal Jurnal Riset Kesehatan Poltekkes Depkes Bandung Jaringan Laboratorium Medis
Yuliansyah Sundara Mulia
Poltekkes Kemenkes Bandung
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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ANALISIS POTENSI ANTIBIOTIKA BERDASARKAN KONSENTRASI HAMBAT MINIMAL DAN KONSENTRASI BAKTERISIDAL MINIMAL KLORAMFENIKOL DAN AMOKSISILIN TERHADAP SALMONELLA TYPHI Deny Rudiansyah; Asep Dermawan; Yuliansyah Sundara Mulia
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 13 No 1 (2021): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.431 KB) | DOI: 10.34011/juriskesbdg.v13i1.1842

Abstract

Infectious sicknesses are as yet one of the medical issues on the planet, including Indonesia. Different sorts of anti-toxins have been utilized to treat irresistible sicknesses. One illustration of an irresistible sickness brought about by microorganisms is typhoid fever or enteric fever brought about by Salmonella typhi (S.typhi.). Since 1948, chloramphenicol has been the medication of decision for S.typhi contamination. Objective: To think about and decide the anti-microbial power of chloramphenicol and amoxicillin against S.typhi. Exploration is expected to discover other anti-infection treatment alternatives that are more touchy. One of them is by directing this examination with the KHM and KBM tests. Methods: To see the potential for anti-microbials, the Minimum Inhibitory Concentration (MIC) was tried utilizing the cylinder weakening strategy and the Minimum Kill Concentration (KBM) utilizing the Kirby Bauer technique. This examination is a kind of in vitro lab test research. Information examination utilizing SPSS application with Mann Whitney U Test. Results: The examination showed that chloramphenicol had anti-infection power with a MIC worth of 12.50 g/ml, while amoxicillin had anti-infection potential with a MIC worth of 6.25 g/ml. The MBC an incentive for chloramphenicol and amoxicillin has a similar worth of 25 g/ml. Conclusion: There is no distinction in MIC and MBC esteems ​​between the anti-toxins chloramphenicol and amoxicillin against S.typhi. Amoxicillin can be utilized as an elective medication for the treatment of typhoid fever disease.
PENGARUH INHIBITOR SISTEIN PADA FERMENTASI BIJI KAKAO TERHADAP KANDUNGAN POLIFENOL Dewi Nurhayati; Yuliansyah Sundara Mulia; Amadda Fadhila Azhiima
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 14 No 1 (2022): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.39 KB) | DOI: 10.34011/juriskesbdg.v14i1.2021

Abstract

Obesity is a condition of excessive accumulation of fat in the body. Dietary fiber and anthocyanins have beneficial effects on obesity. The aim was to determine the effect of the product's formulation of oat flour and fermented glutinous black rice on organoleptic aspects, dietary fiber content, and anthocyanin content. This research design used an experimental study with a completely randomized design. This research method used a hedonic test on 30 panelists to determine organoleptic properties, pH differential for anthocyanin levels, and enzymatic gravimetric for dietary fiber levels. The formulation between oat flour and fermented glutinous black rice obtained was F1 (30:70), F2 (50:50), and F3 (70:30). F2 product has the best rating on all organoleptic aspects, including color, aroma, texture, taste, and overall. The results of the Kruskal Wallis test showed a significant difference in the texture aspect of the product. The anthocyanin content in the product is 5.72 mg/100 grams, and the fiber content in the product is 7.36%. It is hoped that further research will be carried out to determine the effectiveness of oat fermented glutinous black rice cookies on samples with obesity.
Analisis Cost Effectiveness Media Alternatif Berbasis Limbah Kulit dan Kepala Udang Dibandingkan Sabouraud Dextrose Agar untuk Pertumbuhan Trichophyton rubrum Afik Abdurrahman; Sulaeman; Ira Gustira Rahayu; Yuliansyah Sundara Mulia
Jaringan Laboratorium Medis Vol 8 No 2 (2026): November 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i2.15088

Abstract

Dermatophytosis is one of the most common superficial fungal infections and remains a significant public health concern, with Trichophyton rubrum being one of its primary causative agents. Sabouraud Dextrose Agar (SDA) is the standard medium widely used for dermatophyte culture, however, its production cost is relatively high, creating a need for more economical alternatives. Shrimp shell and head hydrolysate contains proteins, peptides, and amino acids that have the potential to be utilized as the basis for an alternative culture medium. This study aimed to evaluate the effectiveness of shrimp waste hydrolysate media (40%, 50%, 60%, 70%, 80%) in supporting the growth of Tricophyton rubrum and to analyze differences in cost effectiveness compared with SDA. A quasi experimental post test only control group design was employed with three replications per medium. Colony diameter was measured every 24 hours for 14 days. Statistical analysis was performed using the Kruskal-Wallis test, while economic evaluation employed the Average Cost Effectiveness Ratio (ACER) and Incremental Cost Effectiveness Ratio (ICER). Shrimp hydrolysate media (40-80%) supported fungal growth from day 2, with faster early phase growth than SDA due to the abundance of small peptides and free amino acids, particularly glutamic acid rapidly metabolized through Nitrogen Metabolite Repression. In the later growth phase, SDA produced more stable and optimal growth owing to its readily available glucose as a primary carbon source, whereas alternative media required sucrose hydrolysis and were affected by nutrient degradation during autoclaving and non standardized pH. No statistically significant differences were observed among media (p = 0.998). Cost effectiveness analysis indicated that the 40% hydrolysate medium was the most cost effective formulation, based on the ratio of production cost to colony growth. Shrimp waste hydrolysate media effectively support Trichophyton rubrum growth, with the 40% concentration representing the most economical option.
Co-Authors Afik Abdurrahman Amadda Fadhila Azhiima Asep Dermawan Deny Rudiansyah Dewi Nurhayati Ira Gustira Rahayu Sulaeman