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All Journal ZIRAA'AH MAJALAH ILMIAH PERTANIAN
Eliyani Eliyani
Faculty Of Agriculture, Universitas Mulawarman, Samarinda, East Kalimantan Province
Agriculture, Biological Sciences & Forestry

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RESPON PERTUMBUHAN ANGGREK TEBU (Grammatophyllum speciosum BLUME) SECARA IN VITRO TERHADAP PEMBERIAN BENZYL AMINO PURIN, KINETIN, NAFTALENA ACETIC ACID DAN EKSTRAK PISANG AMBON DALAM MEDIA DASAR SETENGAH MURASHIGE AND SKOOG Ellok Dwi Sulichantini; Eliyani Eliyani; Alvera Prihatini Dewinazari; Susylowati Susylowati; Agusty Saputra
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 46, No 1 (2021)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v46i1.3941

Abstract

Kalimantan whose existence is increasingly extinct so it needs to be preserved by in vitro propagation. This study aims to determine the effect of a combination of the growth regulators, Benzyl Amino Purin (BAP), Kinetin, Naphthalene Acetic Acid (NAA) and banana extract on the growth of sugar cane orchids in vitro. The basic media used were Half Murashige and Skoog (1/2 MS), on the base media were added 30 g.L-1 sugar, 8 g.L-1 seaweed commercial powder,  plus BAP 3 ppm, Kinetin 3 ppm, NAA 0.5 ppm, and ambon banana extract 100 g.L-1, the acidity (pH) of the media is adjusted to around 5.8. The research design used was a Completely Randomized Design (CRD), each treatment was repeated six times. Data were analyzed using variance analysis and continued with DMRT (Duncan Multiple Range Test) tests with a level of 5%.            The results showed that the half media Murashige and Skoog was good for the propagation of sugarcane orchids.  The addition of BAP growth regulators, Kinetin, NAA, and banana extracts showed a good response to growth culture. The addition of BAP 3 ppm + NAA 0.5 ppm showed the best results for the height increased, leaf number increased, leaf length increased, leaf width increased, and the number of shots increased. The addition of Ambon banana extract to media containing growth regulators tended to decrease the growth response, seen in the parameters of plant height increased, leaf number increased, leaf length increased, leaf width increased, the number of shoots increased, the number of roots increased and in root length increased.
MORFOGENESIS EKSPLAN TUNAS EUCALYPTUS PELLITA F. MUELL SECARA IN VITRO PADA MEDIA MURASHIGE AND SKOOG DENGAN ZAT PENGATUR TUMBUH BENZIL AMINO PURIN Ellok Dwi Sulichantini; Eliyani Eliyani; Alvera Prihatini Dewi Nazari
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 45, No 3 (2020)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v45i3.3481

Abstract

Eucalyptus pellita  is fast growing species and have many advantages such as: pulp and paper industry raw material, building construction, charcoal, and medicine.  The research was carried out to study plant morphogenesis from shoot explant of E. pellita F. Muell by in vitro technique. The experiment was conducted at Tissue Culture Laboratory, Agriculture Faculty of Mulawarman University from March until June, 2016.Single factor experiment was arranged in Completely Randomized Design with 10 replications.  The factor was BAP concentrations (B) consisted of 1.00 mg BAP L-1 (b1); 2.00 mg BAP L-1 (b2); 3.00 mg BAP L-1 (b3); and 4,00 mg BAP L-1 (b4). The explants was innoculated on MS medium supplemented with BAP according to the treatments. Data analyzed by analysis of variance (anova) and continued by Least Significant Difference  (LSD) test  with level 5%. Results of the experiment showed that the BAP concentrations can promote shoot formation of E. pellita.  Statistical analysis indicated that the  effect of BAP concentrations significantly difference on number of shoots at 8 weeks after innoculation (WAI), shoot length, and number of leaves at  4; 6; and 8 WAI, however it’s no significantly difference on shoot formation time and number of shoots at 4 and 6 WAI.  The highest number of shoots,  shoot length, and number of leaves achieved by 4,00 mg BAP L-1.
Co-Authors Agusty Saputra Alvera Prihatini Dewi Nazari Alvera Prihatini Dewinazari Ellok Dwi Sulichantini Susylowati Susylowati