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All Journal BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Jurnal Agroplasma
Sri Suhesti
Balai Penelitian Tanaman Rempah dan Obat, Jl. Tentara Pelajar No. 3 Bogor 16111
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Pengaruh Kombinasi BAP (Benzyl Amino Purine) dan Kinetin Terhadap Multiplikasi Tunas Tanaman Tebu (Saccharum officinarum L.) Varietas AAS Agribun Ramadhan, Mochamad Alif; Bayfuqron, Fawzy Muhammad; Saputro, Nurcahyo Widyodaru; Suhesti, Sri
JURNAL AGROPLASMA Vol 11, No 1 (2024): JURNAL AGROPLASMA VOLUME 11 NO 1 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i1.5342

Abstract

Sugarcane (Saccharum officinarum L.) is a plantation commodity crop and raw material for making sugar. The need for sugar increases every year so it is necessary to provide superior sugarcane seeds in large quantities in a relatively short time. This can be done by propagating using the plant tissue culture method. The research method used was an experimental method using a combined Completely Randomized Design (CRD) with 4 replications and 8 treatments consisting of: Control (P0); 0.5 ppm BAP + 0 ppm kinetin (P1); 1 ppm BAP + 0 ppm kinetin (P2); 2 ppm BAP + 0 ppm kinetin (P3); 0 ppm BAP + 0.1 ppm kinetin (P4); 0.5 ppm BAP + 0.1 ppm kinetin (P5); 1 ppm BAP + 0.1 ppm kinetin (P6); and 2 ppm BAP + 0.1 ppm kinetin (P7). The data then explained the variations and then continued with the DMRT test at the 5% level. The results showed that treatments P3 and P7 showed the best results at the time of shoot emergence. The P3 treatment also showed the best results in the number of shoot (8.25) and number of leaves (16.50). The P7 treatment showed the highest shoot yield (4.58). Keywords: sugarcane, BAP, kinetin, shoot multiplication
Pengaruh Kombinasi 2.4 D (Dichlorophenoxyacetic) dan BAP (Benzyl Amino Purine) pada Media MS (Murashige Skoog) Terhadap Induksi Kalus Tanaman Tebu (Saccharum officinarum L.) Varietas AAS Agribun Fatahillah, Rizky; Rahmi, Hayatul; Saputro, Nurcahyo Widyodaru; Suhesti, Sri
JURNAL AGROPLASMA Vol 11, No 1 (2024): JURNAL AGROPLASMA VOLUME 11 NO 1 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i1.5412

Abstract

Sugarcane (Saccharum officinarum L.) is a plant that is used as the main ingredient in making sugar. Sugar consumption in Indonesia increases from year to year due to population growth. One of the efforts to provide superior seeds in a short time and in large quantities is through tissue culture. This study aims to determine the effect of a combination of 2,4 D (Dichlorophenoxyacetic) and BAP (Benzyl Amino Purine) and determine the best treatment. The research method used is an experimental method using a completely randomized design (CRD) combination with 3 replicates and 9 treatments consisting of: (A); 1 ppm 2,4 D + 0 ppm BAP (B); 2 ppm 2,4 D + 0 ppm BAP (C); 3 ppm 2,4 D + 0 ppm BAP (D); 1 ppm 2,4 D + 0.1 ppm BAP (E); 2 ppm 2,4 D + 0. 1 ppm BAP (F); 3 ppm 2,4 D + 0.1 ppm BAP (G); 1 ppm 2,4 D + 0.2 ppm BAP (H); 2 ppm 2,4 D + 0.2 ppm BAP (I); 3 ppm 2,4 D + 0.2 ppm BAP. The results showed that there was a significant effect of the combination of 2,4-D and BAP on the time of callus appearance and callus diameter in callus induction of Sugarcane (Saccharumofficinarum L.) variety AAS Agribun. The treatment of C (2,4-D 3 ppm + BAP 0 ppm) produces the highest percentage of live explants which is 100%, the fastest callus emergence time which is 15.67 hsi, the largest diameter which is 2.04 cm and the heaviest callus fresh weight with a value of 0.86 grams. Keywords: sugarcane, 2,4 D, BAP, callus induction
Pertumbuhan Tunas Aksilar Tebu Kultivar 'AAS Agribun' dengan Kombinasi BAP dan NAA Secara In Vitro Fajar Hidayat, Muhammad; Kusumarini, Niken; Kartikawati, Andriana; Suhesti, Sri; Sa’adah, Ira Nailas; Kusrinah; Mas'ud, Abdurrahman
Biotik Vol 14 No 2 (2026): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v14i2.35501

Abstract

Sugarcane (Saccharum officinarum L.) is a strategic sugar-producing crop in Indonesia; however, conventional vegetative propagation is limited by slow multiplication rates and the risk of disease transmission. In vitro culture offers an alternative approach for rapid and disease free seedling production. This study aimed to evaluate the effect of Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) combinations on axillary shoot growth of sugarcane cultivar 'Agriculture Acclimated Sugarcane Agribun' (abbreviated as 'AAS Agribun') and to determine the most effective concentration for shoot initiation. The study was conducted from October 2025 to January 2026 at the Laboratorium Unit Pengelola Benih Unggul Pertanian (UPBUP), Pusat Perakitan dan Modernisasi Pertanian (BrMP) Perkebunan, Bogor, West Java. A completely randomized factorial design was applied using BAP (0–2.0 mg/L) and NAA (0–0.5 mg/L) on Murashige and Skoog (MS) medium. Observed parameters included percentage of surviving explants, time of shoot emergence, number of shoots, shoot height, and number of leaves. The results indicated that 0.5 mg/L BAP without NAA produced the most effective response for axillary shoot initiation, as reflected by higher explant survival and improved shoot growth compared to other treatments. This was evidenced by the earliest shoot emergence, observed at 1.75 Weeks After Culture (WAC), and the highest leaf number, averaging 0.96 leaves per plant. These findings suggest that low BAP concentration without auxin supplementation is optimal for early-stage shoot induction of sugarcane cultivar ‘AAS Agribun’. Keyword: Axillary shoots; Plant growth regulators; Sugarcane; Tissue culture.
Co-Authors Bayfuqron, Fawzy Muhammad Fajar Hidayat, Muhammad Fatahillah, Rizky Kartikawati, Andriana Kusrinah Mas'ud, Abdurrahman Niken Kusumarini, Niken Rahmi, Hayatul Ramadhan, Mochamad Alif Sa’adah, Ira Nailas