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I Prihantoro
Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry

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Seleksi Mutan Tanaman Lamtoro (Leucaena leucocephala cv. Tarramba) Tahan Kutu Loncat terhadap Lingkungan Kering pada Rumah Kaca: Mutant Selection of Lamtoro (Leucaena leucocephala cv. Tarramba) on Dry Environment in Green House rini Anggriani; P D M H Karti; I Prihantoro
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 19 No. 3 (2021): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.19.3.90-94

Abstract

Forage is the main source of feed for ruminants. Forage consists of two types, namely grass and legumes. Leguminosa is a type of forage as a source of protein. One type of legume that is well known by breeders in Indonesia is lamtoro (Leucaena leucocephala cv. Tarramba). This study was aimed to produce candidate mutants of lamtoro drought resistant to acid stress conditions. The experiment in this study used an unbalanced completely randomized design with 6 treatments of 740 different replications based on different sources of lamtoro plant mutants at different levels (P0: 0 n= 89, P1: 100 gy n= 82, P2: 200 gy n= 153, P3:300 gy n=120, P4: 400 gy n= 244, P5: 500 gy n= 52). Variables observed included plant height, number of stalks, leaf loss and stem diameter. The results showed that lamtoro plants irradiated with gamma rays at a level of 200 gy-500 gy were significantly higher than 100 gy irradiation at 10 and 12 days watering, but the diameter of the plants was larger at 100 gy irradiation. It can be watering intervals of 10 days and 12 days on the parameters of height and stem diameter showed that gamma rays irradiation of 400 gy resulted in dry-resistant lamtoro mutant candidates under acid stress condition. Key words: gamma rays, lamtoro mutant, watering interval
Morfologi Mutan Alfalfa (Medicago sativa L.) Hasil Iradiasi Sinar Gamma Pada Cekaman Kering: Mutant Morphology of Alfalfa (Medicago sativa L) Result of Gamma Ray Irradiation in Dry Stress Desima Natalia Harianja; P D M H Karti; I Prihantoro
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 19 No. 2 (2021): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.19.2.59-65

Abstract

Alfalfa in dry stress conditions cannot grow optimally. Drought inhibits growth and reduces alfalfa production in many cultivation systems. Plants breeding with gamma-ray irradiation and in vitro selection using PEG found somaclonal variants adapted to grow well in drought conditions. This study aims to determine the type of mutation with the best morphology in dry stress conditions. Alfalfa mutants resulting from gamma irradiation were sub-cultured in vitro. Alfalfa mutants cultured in PEG medium, according to treatments. This experiment used completely randomized factorial design with 2 factors, namely the gamma-ray irradiation doses (0 Gy, 100 Gy, 200 Gy, 300 Gy, 400 and 500 Gy), and PEG concentrations (5%, 10%, 15%, and 20% ). The variables observed were viability, plant height, number of leaves, wet weight, medium shrinkage, leaf wilting, and leaf color. The results showed that the gamma-irradiated mutant had a better (p<0.05) morphology than the control (0 Gy) in the face of dry stress conditions. Combination of 500 Gy and 20% PEG concentration resulted the best type of mutant. Key words: alfalfa, in vitro, gamma rays, PEG
Tingkat Toleransi Tanaman Lamtoro mini (Desmanthus virgatus) terhadap Cekaman Salinitas melalui Teknik Kultur Jaringan: Tolerance Level of Desmanthus virgatus to Salinity Stress through Tissue Culture Techniques Cindy Hardianti Nufus; I Prihantoro; P D M H Karti
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 1 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.1.7-13

Abstract

Desmanthus virgatus has high potential as a source of animal feed and has the opportunity to develop as forage crops in saline areas. This study aimed to obtain information on the tolerance level of D.virgatus to salinity stress at different levels using tissue culture techniques. The experimental design used was a completely randomized design (CRD) with 5 treatments and 25 replications. The treatments were P0 (NaCl 0 ppm), P1 (NaCl 2500 ppm), P2 (NaCl 5000 ppm), P3 (NaCl 7500 ppm), and P4 (NaCl 10000 ppm). The variables observed were plant height, number of compound leaves, number of leaf twigs, leaf color, leaf loss, and plant viability. The results showed that the addition of NaCl with a level of more than 2500 ppm had a significant (p<0.05) effect on plant height, number of compound leaves, number of leaf twigs, and leaf color, but had no significant effect on leaf loss and plant viability. It is concluded D.virgatus had a tolerance level of salinity stress up to 2500 ppm.
Optimasi Penggunaan Mutagen Kolkisin untuk Peningkatan Produktivitas Tanaman Stylo (Stylosanthes guianensis (Aubl.) Sw.): Optimalization of Colchicine Mutagen Utilization to Increase Stylo Productivity (Stylosanthes guianensis (Aubl.) Sw.) Nindi Yulia; I Prihantoro; P D M H Karti
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 1 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.1.19-24

Abstract

Colchicine is a chemical that induced mutation in plants through doubling of plant chromosomes so that polyploid plants are produced which have a larger size than diploid plants. This study aimed to determine the optimal level and soaking time of colchicine to increase the productivity of stylo plants. This study used a Completely Randomized Factorial Design (CRD) with two factors and six replications. Factor A is a the colchicine level of 0 ppm, 25 ppm, 50 ppm, 75 ppm and 100 ppm. Factor B is the soaking time of 12 hours, 24 hours and 36 hours. The variables observed were vertical height, stem diameter, leaflets length, leaflets width and number of trifoliate leaves. The results showed that the colchicine at a level of 25 ppm with soaking time of 12 hours had a significant effect (p<0.05) on the increase in plant vertical height, stem diameter, and leaf width by 75.62 cm, 3.08 mm and 0.90 cm, respectively. The number of trifoliate leaves from the colchicine treatment and the length of the leaflets were not significantly different. The conclusion of this research is colchicine level of 25 ppm and soaking time of 12 hours can increase plant vertical height, stem diameter and leaf width on stylo plants aged 14 WAP (Weeks After Planting). Key words: colchicine level, productivity, soaking time, stylo
Efektivitas Berbagai Produk Fungi Mikoriza Arbuskula Dalam Meningkatkan Produktivitas Stylosanthes guianensis Pada Tanah Masam: The Effectiveness of Various Arbuscular Mycorrhizal Fungi Products in Increasing Productivity of Stylosanthes guianensis on Acid Soil Agam Rizki; Panca Dewi Manu Hara Karti; I Prihantoro
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 3 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.3.89-94

Abstract

This study aimed to evaluate the effectiveness of Arbuscular Mycorrhizal Fungi (AMF) against Stylosanthes guianensis plants on acid soils. The research was conducted in the greenhouse of the Agrostology Laboratory of IPB. The research design used was a completely randomized design with 5 treatments and 8 replications, treatments were different types of AMF, without AMF (F0), commercial AMF (FU), AMF product IPB 1 (EM1), AMF product IPB 2 (EM2) and AMF product IPB 3 (EM3). AMF was given as much as 20 grams per planting hole. The given fertilizers were manure and NPK fertilizer. The treatment without AMF was given 100% of the recommended dose, while treatment with AMF was only given 50% of the dose. The results showed that there was no significant difference between treatments on growth variables (plant height, number of trifoliate leaves, number of branches, stem diameter and leaf chlorophyll). The type of AMF affected the fresh shoot production, and the highest was at EM1 (38.03 grams), as well as the number of root infections had a significant effect, and the highest production was at EM1 (77%). The conclusion in this study was that the use of AMF could reduce the use of manure and NPK 50% from the recommended dose, could increase the growth and productivity of Stylosanthes guianensis on acid soils and AMF product IPB 1(EM1) gave the best results. Key words: root infection, marginal soil, leguminous, Stylosanthes guianensis
Co-Authors Cindy Hardianti Nufus Harianja, Desima Natalia Nindi Yulia P D M H Karti Panca Dewi Manu Hara Karti rini Anggriani Rizki, Agam