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All Journal Majalah Ilmiah Peternakan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Ilmu Nutrisi dan Teknologi Pakan
Harianja, Desima Natalia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Morfologi Mutan Alfalfa (Medicago sativa L.) Hasil Iradiasi Sinar Gamma Pada Cekaman Kering: Mutant Morphology of Alfalfa (Medicago sativa L) Result of Gamma Ray Irradiation in Dry Stress Desima Natalia Harianja; P D M H Karti; I Prihantoro
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 19 No. 2 (2021): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.19.2.59-65

Abstract

Alfalfa in dry stress conditions cannot grow optimally. Drought inhibits growth and reduces alfalfa production in many cultivation systems. Plants breeding with gamma-ray irradiation and in vitro selection using PEG found somaclonal variants adapted to grow well in drought conditions. This study aims to determine the type of mutation with the best morphology in dry stress conditions. Alfalfa mutants resulting from gamma irradiation were sub-cultured in vitro. Alfalfa mutants cultured in PEG medium, according to treatments. This experiment used completely randomized factorial design with 2 factors, namely the gamma-ray irradiation doses (0 Gy, 100 Gy, 200 Gy, 300 Gy, 400 and 500 Gy), and PEG concentrations (5%, 10%, 15%, and 20% ). The variables observed were viability, plant height, number of leaves, wet weight, medium shrinkage, leaf wilting, and leaf color. The results showed that the gamma-irradiated mutant had a better (p<0.05) morphology than the control (0 Gy) in the face of dry stress conditions. Combination of 500 Gy and 20% PEG concentration resulted the best type of mutant. Key words: alfalfa, in vitro, gamma rays, PEG
EVALUASI DAN SELEKSI MUTAN ALFALFA HASIL IRADIASI SINAR GAMMA PADA CEKAMAN ABIOTIK HARIANJA D.N.; P.D.M.H. KARTI; I. PRIHANTORO
Majalah Ilmiah Peternakan Vol 25 No 1 (2022): Vol 25, No 1 (2022)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MIP.2022.v25.i01.p09

Abstract

Alfalfa is a leguminous protein source with high nutritional value and economic value. Abiotic stress inhibits growth and reduces alfalfa production in many aquaculture systems. New varieties were developed by gamma-ray mutation induction. The selection was carried out to obtain superior abiotic stress-tolerant plants. This study aims to evaluate and select alfalfa mutants under abiotic stress conditions. The design used in this study was a comple- tely randomized design (CRD) with a factorial pattern. The first factor is the type of acid-tolerant mutants, namely A44, A54, A35, and A45. The second factor is the PEG level which is 10%, 15%, and 20%. The variables observed included viability, plant height, number of leaves, leaf wilting, leaf color, media shrinkage, and wet weight. Alfalfa mutants resulting from acid-tolerant gamma irradiation were evaluated under dry stress conditions. The results showed that mutation induction resulted in various responses to growth. Gamma-ray irradiation produces plants that are tolerant to abiotic stress conditions. From the selection results obtained superior mutant candidates that are tolerant of acid stress and dry conditions. The A44 mutant had better tolerance to dry stress than others and became an acid- and dry-tolerant alfalfa mutant.
Seleksi In Vitro Mutan Alfalfa Hasil Iradiasi Sinar Gamma pada Cekaman Asam Harianja, Desima Natalia; Manuhara Karti, Panca Dewi; Prihantoro, Iwan
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (623.625 KB) | DOI: 10.33772/jitro.v9i1.19445

Abstract

ABSTRAKAlfalfa  (Medicago sativa L.) telah digunakan sebagai makanan ternak yang efektif dan bergizi. Kondisi cekaman asam membatasi pertumbuhan dan produksi alfalfa dalam banyak sistem budidaya. Perbaikan genetik melalui iradiasi sinar gamma dan seleksinya dengan AlCl3+ dihasilkan keragaman genetik toleran cekaman asam. Penelitian ini bertujuan untuk mengetahui karakteristik mutan alfalfa hasil iradiasi sinar gamma yang diberi AlCl3+ sebagai simulasi cekaman asam. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) pola faktorial. Faktor pertama adalah dosis iradiasi sinar gamma yaitu 0Gy, 100Gy, 200Gy, 300Gy, 400Gy dan 500Gy. Faktor kedua adalah level AlCl3+ yaitu 100ppm, 200ppm, 300ppm, 400ppm dan 500ppm. Variabel yang diamati meliputi viabilitas, tinggi vertikal, jumlah daun, kelayuan daun, penyusutan media, bobot basah, warna daun dan perubahan pH. Hasil penelitian menunjukkan bahwa mutan alfalfa teriradiasi sinar gamma memberikan karakteristik terbaik dibanding alfalfa tanpa iradiasi sinar gamma terhadap peningkatan level AlCl3+. Hingga abang batas tertentu peningkatan dosis sinar gamma dapat meningkatkan pertumbuhan dan ketahanan alfalfa pada kondisi tercekam asam. Dapat di simpulkan bahwa induksi mutasi dengan iradiasi sinar gamma dapat dihasilkan mutan alfalfa yang tahan pada kondisi tercekam asam hingga level AlCl3+ 500ppm, dengan dosis iradiasi sinar gamma terbaik pada dosis 400Gy.Kata Kunci: alfalfa, sinar gamma, alcl3+, in vitroIn Vitro Selection of Mutants Alfalfa Result in Gamma-Ray Irradiation in Acid StressABSTRACTAlfalfa (Medicago sativa L.) has been used as an effective and nutritious animal feed. Acid stress conditions limit alfalfa growth and production in many cultivation systems. Genetic improvement through gamma-ray irradiation and its selection with AlCl3+ resulted in acid stress-tolerant genetic diversity. This study aims to determine the characteristics of alfalfa mutants resulting from gamma irradiation given AlCl3+ as a simulation of acid stress. The design used in this study was a completely randomized design (CRD) with a factorial pattern. The first factor is the gamma-ray irradiation dose, namely 0Gy, 100Gy, 200Gy, 300Gy, 400Gy, and 500Gy. The second factor is the level of AlCl3+, namely 100ppm, 200ppm, 300ppm, 400ppm and 500ppm. The variables observed included viability, vertical height, number of leaves, leaf wilting, media shrinkage, wet weight, leaf color, and changes in pH. The results showed that the alfalfa mutant irradiated with gamma rays gave the best characteristics compared to alfalfa without gamma irradiation to increase the level of AlCl3+. Up to a certain threshold, increasing the dose of gamma rays can increase the growth and resistance of alfalfa under acid stress conditions. It can be concluded that mutation induction with gamma-ray irradiation can produce alfalfa mutants that are resistant to acid stressKeywords: alfalfa, gamma rays, alcl3+, invitro
Co-Authors I Prihantoro I. PRIHANTORO Iwan Prihantoro P D M H Karti P.D.M.H. Karti Panca Dewi Manu Hara Karti