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Rusmiati Suprihatin
Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta 55281
Chemical Engineering, Chemistry & Bioengineering Chemistry

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THE INFLUENCE OF ORGANIC SOLVENT PROTEIN PRECIPITATION ON SDS PAGE PROTEIN PROFILE IN SERUM Tri Joko Raharjo; Rusmiati Suprihatin; Deni Pranowo
Indonesian Journal of Chemistry Vol 7, No 3 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.948 KB) | DOI: 10.22146/ijc.21680

Abstract

A study on the influence of organic solvent protein precipitation to the profile of the serum protein has been accomplished. The expected conditions were precipitation of abundant proteins present in serum result in increasing relative concentration of minor protein which can be useful for sample preparation for biomarker studies. The serum were precipitated with various diluted (<10%) acetonitrile, methanol and ethanol followed by SDS-PAGE analysis of the supernatant in order to investigate the protein profile. There were no linear relationship between solvent concentration and the number of protein bands. However, an optimal condition of precipitation was found which is by methanol 0.1%.
Co-Authors Deni Pranowo Tri Joko Raharjo