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Robert Verpoorte
Division of Pharmacognosy, Section Metabolemics, Institute of Biology, Leiden University, Gorlaeus Laboratories, PO Box 9502, 2300 RA, Leiden, The Netherlands
Chemical Engineering, Chemistry & Bioengineering Chemistry

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CALLUS INDUCTION AND PHYTOCHEMICAL CHARACTERIZATION OF Cannabis sativa CELL SUSPENSION CULTURES Tri Joko Raharjo; Ogbuadike Eucharia; Wen-Te Chang; Robert Verpoorte
Indonesian Journal of Chemistry Vol 6, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1101.342 KB) | DOI: 10.22146/ijc.21776

Abstract

Callus of Cannabis sativa has been successfully induced from C. sativa explants and seedings. It seems that flowers are the best explant for callus induction and induction under light also give better results than induction in dark. Four cell culture lines were established from flower induced callus. Phytochemical profiles of C. sativa suspension cell cultures were investigated using HPLC and 1H-NMR. Cannabinoids and phenolic compounds related to cannabinoids such as flavonoids could not be found in the cell suspension cultures and there is no major chemical difference between the cell lines though they can visually be distinguished by their colors. Only in one cell line some aromatic compounds in the water/methanol extract could be observed in the 1H-NMR. Further investigations showed that none of these compounds are flavonoids. It seems that lack of cannabinoids in the cell cultures is related to lack of polyketide synthase activity.
Co-Authors Ogbuadike Eucharia Tri Joko Raharjo Wen-Te Chang