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All Journal Indonesian Journal of Chemistry
Siti Kholijah Abdul Mudalip
Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak 26300, Gambang, Pahang, Malaysia
Chemical Engineering, Chemistry & Bioengineering Chemistry

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Comparative Evaluation of Protease Extraction from Leaves of Syzygium polyanthum Noorhafiza Yahya; Zatul Iffah Mohd Arshad; Siti Kholijah Abdul Mudalip; Siti Zubaidah Sulaiman; Rohaida Che Man; Shalyda Md Shaarani; Mohd Hairul Ab. Rahim
Indonesian Journal of Chemistry Vol 19, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.768 KB) | DOI: 10.22146/ijc.40860

Abstract

In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogen-bond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods.
Semirefined Carrageenan (SRC) Film Incorporated with α-Tocopherol and Persicaria minor for Meat Patties Application Khadijah Husna Abd Hamid; Wan Amnin Wan Yahaya; Neenasha Bebe Mohd Nor; Alyaa Syahierra Ghazali; Siti Kholijah Abdul Mudalip; Norashikin Mat Zain; Maria Pilar Almajano; Nurul Aini Mohd Azman
Indonesian Journal of Chemistry Vol 19, No 4 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.799 KB) | DOI: 10.22146/ijc.40884

Abstract

Semirefined carrageenan (SRC) plasticized with glycerol (G) and incorporated with antioxidants of 0.4% (v/v) of α-tocopherol and Persicaria minor (PM) extract was successfully developed. The objective of this study is to analyze the antioxidant effect of active packaging films from semirefined carrageenan incorporated with α-tocopherol and Persicaria minor on meat patties. Total phenolic content and antioxidant activity of α-tocopherol and PM extract were measured. The effects of α-tocopherol and PM extract incorporated with SRC-based films on meat patties were evaluated using thiobarbituric acid reactive substance (TBARS) assay, metmyoglobin assay, and pH value for 14 days of storage. The films with 0.4% (v/v) of α-tocopherol and PM extract exhibited a lower lipid oxidation in meat patties compared with that of control (SRC film only, p < 0.05). Also, a brown color development of the meat patties of less than 50% was observed at the end of the 14-day storage. Meanwhile, the pH values for all samples decreased throughout the storage period with the SRC+G+α-tocopherol film showed the highest pH value. Hence, the formulation of SRC film with α-tocopherol or PM extract could be used as an alternative packaging for extending the shelf life of food product with high fat content.
Co-Authors Alyaa Syahierra Ghazali Khadijah Husna Abd Hamid Maria Pilar Almajano Mohd Hairul Ab. Rahim Neenasha Bebe Mohd Nor Noorhafiza Yahya Norashikin Mat Zain Nurul Aini Mohd Azman Rohaida Che Man Shalyda Md Shaarani Siti Zubaidah Sulaiman Wan Amnin Wan Yahaya Zatul Iffah Mohd Arshad