Dian Susanthy
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara BLS 21, Bulaksumur, Yogyakarta 55281, Indonesia

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Antibacterial Activity of Silver Nanoparticles Capped by p-Aminobenzoic Acid on Escherichia coli and Staphylococcus aureus Dian Susanthy; Sri Juari Santosa; Eko Sri Kunarti
Indonesian Journal of Chemistry Vol 20, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (25.72 KB) | DOI: 10.22146/ijc.44652

Abstract

This paper describes the antibacterial performance of silver nanoparticles (AgNPs) which have been synthesized by using p-aminobenzoic acid as reducing and stabilizing agent simultaneously. The silver nitrate with various concentrations was reacted with pH 11-adjusted p-aminobenzoic acid with a concentration of 5 Ɨ 10–3 mol L–1 for 30 min in a boiling water bath. The synthesized AgNPs were characterized by UV-Vis spectrophotometry, Transmission Electron Microscope (TEM), and Particle Size Analyzer (PSA). The antibacterial performance of the synthesized AgNPs was evaluated by agar well diffusion method on Escherichia coli and Staphylococcus aureus. The higher silver nitrate concentration, the bigger the nanoparticle size, the wider particle size distribution, and the higher number of AgNPs formed. AgNPs synthesized from higher silver nitrate concentration had higher antibacterial activity. It is an indication that the antibacterial activity of AgNPs is mainly controlled by the silver ion concentration which influences the AgNPs particle size and existence of silver ion in the AgNPs colloidal solution