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All Journal ANNALES BOGORIENSES
Sukma Nuswantara
Research Centre for Biotechnology, Indonesian Institute of Sciences (LIPI), Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Conservation of Major L1 and Variability of Minor L2 Capsid Late Protein Genes in Human Papillomavirus of Indonesia Variants Sukma Nuswantara; Titik K Prana; Dwi Wulandari; Henni Widyowati; Vera Anzela; Dea Levy; Petrus Cahyadi; Lukas D Tjandra
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (898.192 KB) | DOI: 10.14203/ann.bogor.2010.v14.n2.1-8

Abstract

Human Papilloma Virus  (HPV) has an out standing feature for its vast intraspecies variability. Of all known 100 types or  more, 15 t ypes of them are classified as high risk  because of their occurrence  in  more than 95% of cervical cancer cases. Among all genes in their genome, E6 and E7 genes are considered oncogenes and have close relevance with their pathogenicity, whilst L1 and L2 ge nes produce capsid proteins that directly interact with their  host  receptors.  Considering  the  importance  of  L1  and  L2  in  host-receptor  relationship,  we  tried  to investigate their molecular variability thereby uncover their specificity as Indonesian variant s. Here we reported about the conservation of L1 minor capsid protein and variability of L2 capsid protein among high-risk types Human Papilloma Virus (HPV). The results indicated that L1 DNA was relatively more conserved than its L2 counterpart Also it was indicated that the middle part of either L1 or L2 CDS‟ showed more DNA variability than those  at  their  upstream  sequences.  It  is  concluded  that  L2  middle  sequences  are  important  factors  for intraspecific variations found in HPV of Indonesian variants.   Keywords: Human Papilloma Virus (HPV), L1 gene, L2 gene, cervical cancer, high risk HPV
Enhancement of β-Glucosidase Activity in Penicillium sp. by Random Mutation with Ultraviolet and Ethyl Methyl Sulfonate Vilya Syafriana; Sukma Nuswantara; Wibowo Mangunwardoyo; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.422 KB) | DOI: 10.14203/ann.bogor.2014.v18.n2.27-33

Abstract

The genus Penicillium has a potential ability to produce β-glucosidase. The aim of the study was to improve the β-glucosidase activity of Penicillium sp. ID10-T065 with physical (Ultraviolet = UV), chemical (Ethyl Methyl Sulfonate = EMS), and combined mutation (UV-EMS). The spores of Penicillium sp. ID10-T065 were exposed into UV irradiation for 3 minutes with dose of 0.1 J/cm2 and 13 cm of distances. Chemical mutation was done by treated spores into 3% of EMS solution for an hour. Combined mutation of UV and EMS were also performed by UV for 3 minutes (0.1 J/cm2, 15 cm) and continued with soaking into 2-3% of EMS solution. The developed mutants were screened, selected and assayed. Comparison of enzyme activities with the wild- type (1.78 U/ml), mutant UV13 (5.53 U/ml) showed a 3.1 fold increase; mutant EM31 (4.26 U/ml) showed a 2.4 fold increase. Meanwhile, mutant UM23 obtained from the multiple exposures showed a decreased activity (1.75 U/ml). Mutant UV13 showed the best enzyme activity to be considered as a potential strain for β-glucosidase producer. This result needs to be further elaborated especially on its genetic stability studies in order for the ascertained as a stable mutant.
Co-Authors Dea Levy Dwi Wulandari Henni Widyowati Lukas D Tjandra Petrus Cahyadi Puspita Lisdiyanti Syafriana, Vilya Titik K Prana Vera Anzela Wibowo Mangunwardoyo