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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Bioma : Jurnal Biologi dan Pembelajaran Biologi Abditani : Jurnal Pengabdian Masyarakat Sciscitatio : Journal for Biological Science Environmental Science and Engineering Conference Proceeding
Catarina Aprilia Ariestanti
Department Of Biology, Faculty Of Biotechnology, Universitas Kristen Duta Wacana, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Public Health Other

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Journal : Sciscitatio : Journal for Biological Science

 1 
Detection and Identification of Microbial Contaminant in Bakery Products in Yogyakarta City, Indonesia Ferdian, Evelyn; Ariestanti, Catarina Aprilia; Budiarso, Tri Yahya
SCISCITATIO Vol 1 No 1 (2020): Volume 1, Number 1, January 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.11.13

Abstract

Bread has been chosen as an alternative food because of its availability. In Indonesia, consumptionof breads is increased due to the change of diet pattern into packed and ready-made meals. Therefore, it isimportant to raise the awareness of bakery products quality. The purpose of this study was to detect andidentify the microbial contaminant in bakery products in Yogyakarta City, Indonesia. Bacterial coloniesfrom expired bakery products were isolated into pure isolate then confirmed by API Staph and PolymeraseChain Reaction (PCR) method. The results showed there were five kinds of gram-positive bacteria. Isolatedbacteria identified by API were Bacillus cereus (52.8%), Bacillus subtilis (97.7%), Staphylococcus aureus (97.7%),Staphylococcus epidermidis (97,9%) and Staphylococcus saprophyticus (72,2% and 61,8%).
Isolation and Identification of Biofilm-Forming Staphylococcus Aureus in Commercial Cow Milk Products Yambise, Dominggas; Ariestanti, Catarina Aprilia; Budiarso, Tri Yahya
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.33

Abstract

Food poisoning caused by the contamination from Staphylococcus aureus are frequently found in foodespecially in dairy products. Pasteurization process in milk production was not enough to kill S. aureus becauseit formed biofilm that could survive in high temperature. This research aimed to study the presence of biofilmforming S. aureus in samples from packed commercial milk products in Yogyakarta City, Indonesia. Twentyisolates from dairy products were grown in Brain Heart Infusion (BHI) broth then inoculated into Braid-ParkerAgar (BPA) medium to get the candidate of S. aureus isolates. These isolate candidates were selected usingMannitol Salt Agar (MSA) and Congo Red Agar (CRA) medium. Another selection was done by carbohydratefermentation analysis and confirmed using API STAPH. Confirmation analysis showed that eight isolateswere identified as S. aureus. Another two isolates were identified as S. xylosus and S. haemolyticus. Therefore,it indicated the presence of Staphylococcus aureus as contaminant in dairy products.
The Potency of Corn (Zea Mays) Cob Waste as a Prebiotic Candidate to Support the Growth of Bifidobacterium longum: a Preliminary Study Aprilia Ariestanti, Catarina; Ananda Sejati1, Ribka; Thea Setyaratri, Fransiska; Angel Meliana, Florencia
SCISCITATIO Vol. 3 No. 1 (2022): Volume 3, Number 1, January 2022
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2022.31.87

Abstract

Corn cob is one of the most common agricultural wastes in Indonesia with high content of xylan. Xylan can be used to produce xylitol which cannot be digested by human digestive enzymes thus, make it a potential candidate of prebiotic. This preliminary research aimed to study the potency of corn cob in supporting the growth of Bifidobacterium longum. Corn cob was produced into powder and used as the carbon source in the growth medium of B. longum. Total plate count method was done to count the growth of B. longum in various concentrations of bacteria solution. The result showed that crude corn cob powder can support the growth of B. longum from bacterial enumeration with the value of 2.01 x 106 CFU/mL. The study indicated that corn cob has a potential to be used as prebiotic.
Co-Authors Ananda Sejati1, Ribka Angel Meliana, Florencia Aniek Prasetyaningsih Dan Daniel Pandapotan Djoko Rahardjo Ferdian, Evelyn Guruh Prihatmo Hardo Firmana Given Grace Manik Irene Agni Teatrawan Kukuh Madyaningrana Kukuh Madyaningrana Lavenia, Jovita Nurlia Widayanti Rossalina Christanti Safriana Nata Wijaya Seta Nurhayati Mularum Thea Setyaratri, Fransiska Tri Yahya Budiarso TRI YAHYA BUDIARSO Wulan Sari Sinaga Yambise, Dominggas