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All Journal METAMORFOSA Journal of Biological Sciences Quagga Jurnal Florea Pro-Life Edumatsains BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) BIOTROPIC The Journal of Tropical Biology JURNAL GALUNG TROPIKA Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Entomologi Indonesia BUANA SAINS Sciscitatio : Journal for Biological Science Indigenous Biologi Mediagro SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Journal Science of Biodiversity Journal Science of Biodiversity Bioma : Jurnal Biologi dan Pembelajaran Biologi
Kukuh Madyaningrana
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana, Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Mathematics Physics Other

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Potency of Citrus reticulata Peel Extract as Active Compound of NonAlcohol Based Gel Hand Sanitizer Santoso, Julyana Dika; Prasetyaningsih, Aniek; Madyaningrana, Kukuh
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.32

Abstract

Hand sanitizer is nowadays known as a part of one’s personal hygiene kit because of its practical use andeffectivity against skin microbes, such as Staphylococcus aureus. The common commercial hand sanitizers are madefrom alcohol which may have negative side effect like skin irritation. Utilization of active compounds from a plantthat has antibacterial compounds might be applied to the substitution of alcohol in the formulation of hand sanitizer.In this study, phytochemical compounds from Citrus reticulata and Citrus aurantifolia peel extracts were testedqualitatively and their antibacterial acitivity on Staphylococcus aureus were tested using disk diffusion method. Theresults showed that Citrus reticulata peel extract at the concentration of 70% was the most effective concentration ininhibiting Staphylococcus aureus. This certain concentration of Citrus reticulata peel extract was then chosen in the gelhand sanitizer formulation. Application of gel hand sanitizer with Citrus reticulata peel extract as its active compoundon the hands of respondents were also observed to inhibit bacterial growth. The use of peel extracts from Citrusspp, especially from Citrus reticulata might be potential in the formulation of non-alcohol based gel hand sanitizer.
Antibacterial Activity of Lime Peel and Lemongrass Extract as Active Ingredients for Spray Hand Sanitizer Djima, Elza Gitaa Eirene Djima; Prasetyaningsih, Aniek; Madyaningrana, Kukuh
SCISCITATIO Vol. 2 No. 1 (2021): Volume 2, Number 1, Januari 2021
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hand sanitizers are widely used as an alternative way to maintain hand hygiene from the presence ofpathogenic bacteria, such as Staphylococcus aureus. In general, excessive use of alcohol as the main ingredientin hand sanitizers might cause skin irritation. Utilizing antibacterial activity of plants as a component in handsanitizer could be used as a substitution for alcohol. The aim of this study was to determine the potentialantibacterial activity of lime peel and lemongrass extracts as active ingredients for hand sanitizer. Methodused in this research included extraction using maceration, qualitative phytochemical test, antibacterialassay, and formulation of spray hand sanitizer and quality test of spray hand sanitizer. In this study alkaloid,flavonoid, saponin, tannin and terpenoid were found as phytochemical content of lime peel, while lemongrassextract contain fla vonoid, tanin, saponin and steroid. Compared to lemongrass extract, lime peel extract withconcentration 40%showed the optimum inhibition zone of S. aureus and chosen as active ingredient in sprayhand sanitizer formulation. Formulated spray hand sanitizer with lime peel extract was able to inhibit thegrowth of S. aureus bacteria on hand palm.
THE EFFECT OF KESAMBI BARK EXTRACT ON MICE LYMPHOCYTE COUNT AND SPLEEN INDEX Christine Stefani Tamelan; Kukuh Madyaningrana; Vinsa Cantya Prakasita
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 2 (2022): Februari 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.6039

Abstract

Kesambi is a plant widely used by people of Timor Island. Flavonoid content of Kesambi is potential to be used as immunomodulators. This study aimed  to study the effect of Kesambi bark extract on lymphocyte count and spleen index of male mice as an indicator of their immune responses. Research included identification of phytochemical compounds macerated from Kesambi bark, lymphocytes count and spleen index calculation. Mice injected with sheep erythrocytes were treated orally with three doses of Kesambi extract (0.113 mg/g BW, 0.491 mg/g BW, and 0.712 mg/g BW) for 10 days. Lymphocyte count using blood smear method was carried out on day 0, 5 and 10, while lymphocyte count using hematology analyzer and spleen index measurement were performed after mice sacrificed. Kesambi bark contains alkaloids, flavonoids, tannins, and steroids. Treatment with Kesambi bark extract reduced mice lymphocyte count. Administration of 0.491 mg/gr BW dose reduced the mice lymphocytes count close to the number of lymphocytes in the negative control group. The spleen index of mice had no correlation with changes in the number of lymphocytes. This preliminary study shows a potential use of Kesambi bark extract as a natural immunosuppressant.
Pertumbuhan Cacing Sutra (Tubifex sp) Pada Substrat Lumpur, Pasir, dan Kombinasinya Allan Buddie Santoso; Kukuh Madyaningrana; Kisworo Kisworo
Florea : Jurnal Biologi dan Pembelajarannya Vol 9, No 1 (2022)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v9i1.10451

Abstract

The increase of freshwater aquaculture leads to the increasing demand for silkworms (Tubifex sp) as natural feed. Unfortunately, high demand of silkworm supply is not fully fulfilled yet because its availability is relied on natural catch. Cultivation of Tubifex sp. outside its natural habitats requires good information on substrates and feed. We aimed to study the effect of  mud, sand and their combination use as substrates in supporting Tubifex sp growth. This study used complete randomized design with 5 treatments, namely K1 (100% sand), K2 (100% mud), P1 (combination 75% mud-25%) sand, P2 (combination 50% mud-50% sand), and P3 (combination 25% mud-75% sand) with triplicates on each treatment. Research was conducted for 21 days using fermented chicken manure as feed. Tubifex sp growth was measured on biomass, number of individuals, and average body weight, while pH, temperature, dissolved oxygen and total organic matter were listed as measured environmental parameters. The use of sand and mud combination as substrate was better than a single sand or mud alone to support Tubifex sp growth. Combination of 75% mud and 25% sand showed the best growth of Tubifex sp as observed on biomass value of 13,244 g, 370 number of individuals, and average weight body of 2,741 mg. The application of a combination of sand and mud substrates has a potency to support the cultivation of Tubifex sp outside its native habitat.
POTENSI BIOAKARISIDA EKSTRAK DAUN MIMBA (Azadirachta Indica) DAN BUNGA CENGKEH (Syzygium Aromaticum) TERHADAP TUNGAU PENYEBAB PENYAKIT KREPES PADA JAMUR KUPING Meilani Apra; Aniek Prasetyaningsih; Kukuh Madyaningrana
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 5 No 2 (2021): Januari
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v5i2.2241

Abstract

Wood ear mushrooms is commonly known as one of the cultivated edible mushrooms in Indonesia. The demand for this commodity is still steadily high and leads mushroom farmers to optimize its cultivation. For mushroom farmers in Yogyakarta, the prevalence of krepes disease which is caused by a group of species belongs to Acarina order is still high and leads to total loss of harvest. In order to diminish these Acarina species, the use of chemical acaricides is common because of their effectiveness in eradicating the pests despite their toxic effect to the environment. A strategy to provide environmental friendly acaricides which are extracted from plants is therefore important. This research aimed to study the potency of neem leaves (Azadirachta indica) and clove flowers (Eugenia caryophyllata) extracts as bioacarisides for Acarina species as the causal agent of krepes disease. Neem leaves and cloves flowers were extracted using maceration and soxhlet extraction methods, using 96% methanol as their solvents. Obtained crude extracts would then be subjected to phytochemical analysis using qualitative biochemical tests and GC-MS. The effectivity of extracts as bioacaricides were tested both in in vitro and field scale. In vitro tests showed that neem leaves extract and clove flower extract had LC50 value ​​of 0.22% and 0.48% respectively. Field scale test on mushroom baglogs showed that the efficacy of the neem leaves and clove flower to eradicate acarina were above 50%. Taken together, neem leaves and clove flower extracts are potential to be used as bioacaricides in mushroom farming.
Pemanfaatan Limbah Ampas Coffea Canephora sebagai Pupuk Pendukung Pertumbuhan Altenanthera Sissoo Irene Agni Teatrawan; Kukuh Madyaningrana; Catarina Aprilia Ariestanti; Guruh Prihatmo
BIOMA Vol 7, No 1 (2022): BIOMA: JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v7i1.5822

Abstract

Limbah berupa ampas kopi dapat mencemari lingkungan apabila dibuang tanpa pengolahan. Tujuan penelitian yaitu mempelajari dampak pemberian pupuk ampas kopi Robusta (Coffea canephora) fermentasi dan non fermentasi terhadap pertumbuhan tinggi, jumlah daun, berat basah, bobot kering dan panjang akar dari tanaman Bayam Brasil (Altenanthera Sissoo) dan mengetahui kualitas pupuk ampas kopi berdasarkan kandungan senyawa C, N, P, dan K. Rancangan penelitian yaitu Acak Kelompok (RAK), dianalisis dengan UNIANOVA, uji DMRT dan uji LSD. Hasil penelitian yaitu pupuk ampas kopi robusta (Coffea canephora) yang difermentasi dengan dosis 140 g memiliki pengaruh signifikan dalam mendukung pertumbuhan tanaman bayam Brasil (Altenanthera Sissoo).
Percontohan taman toga serta produksi jamu berbasis tanaman berkhasiat untuk peningkatan kesehatan dan ekonomi masyarakat Djoko Rahardjo; Seta Nurhayati Mularum; Kukuh Madyaningrana; Catarina Aprilia Ariestanti; Aniek Prasetyaningsih; Nurlia Widayanti
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5, No 2 (2022): Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i2.14328

Abstract

Tanaman berkhasiat obat banyak dimanfaatkan oleh masyarakat di Indonesia secara turun-temurun untuk mendukung kesehatan. Inventarisasi dan pemanfaatan beragam tanaman obat mutlak dilakukan untuk pengembangan potensinya dalam menunjang kesehatan. Pemanfaatan jamu sebagai produk olahan tanaman obat mulai mendapatkan perhatian lebih dari pemerintah melalui institusi kesehatan. Pengabdian ini bertujuan untuk membuat percontohan taman tanaman obat keluarga (Toga) di Dusun Ngelosari, Kecamatan Piyungan, Kabupaten Bantul yang memadukan rintisan ekowisata lereng bukit dan pengolahan tanaman obat keluarga. Kegiatan pengabdian ini didasarkan pada analisis situasi dan permasalahan kesehatan di lingkup Puskesmas Piyungan. Berdasarkan identifikasi masalah dan analisis kebutuhan yang dilakukan bersama puskesmas dan kader kesehatan maka pendekatan pengembangan percontohan taman toga, pelatihan dan pendampingan pada kelompok wanita tani (KWT) dipilih sebagai pendekatan yang diharapkan mampu menyelesaikan permasalahan kesehatan dan ekonomi masyarakat. Program terealisir dalam bentuk persiapan dan observasi lokasi taman Toga, pengembangan taman Toga percontohan, pelatihan budidaya tanaman obat, pasca panen dan pengolahan, serta pengemasan dan pemasaran produk herbal. Kegiatan ini mempunyai hasil berupa Taman Toga Ngupoyo Sehat yang menjadi sumber bahan baku produk jamu yang dihasilkan oleh KWT setempat. Selain meningkatkan pendapatan KWT setempat, pelaksanaan program dapat dipergunakan sebagai model sinergisme pengembangan konservasi dan peningkatan kesehatan masyarakat.
Preferensi Media Tumbuh Tungau Penyebab Penyakit Krepes pada Jamur Kuping (Auricularia polythrica) Kukuh Madyaningrana; Meilani Apra
Quagga : Jurnal Pendidikan dan Biologi Vol 13, No 2 (2021): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v13i2.3793

Abstract

Abstrak: Jamur Kuping (Auricularia polytrica) yang banyak dibudidayakan oleh petani jamur di Daerah Istimewa Yogyakarta sering terkendala oleh penyakit dikenal dengan nama setempat sebagai krepes.  Penyakit ini disebabkan oleh organisme dari kelompok tungau (acarina). Identifikasi terhadap penyebab penyakit krepes di pertanian jamur Indonesia ini belum dilakukan secara komprehensif. Identifikasi terhadap tungau penyebab penyakit krepes membutuhkan teknik perbanyakan tungau yang tepat dimana informasi tentang media optimum pertumbuhan tungau menjadi dasar yang penting untuk mendukung teknik kultur tungau yang baik. Penelitian ini bertujuan untuk mempelajari medium optimum untuk perbanyakan kultur tungau. Penelitian ini menggunakan beragam bahan yang kemungkinan menjadi media preferensi tungau untuk tumbuh, baik pada uji medium invitro ataupun pada uji medium lapang (pada baglog jamur). Hasil penelitian menunjukkan bahwa tungau penyebab penyakit krepes pada jamur kuping lebih menyukai tempat hidup pada baglog yang sudah ditumbuhi oleh miselium jamur dan baglog yang sudah menghasilkan badan buah jamur. Hal ini terkait ketersediaan nutrisi yang dimiliki oleh miselium atau badan buah jamur yang telah tumbuh dalam media baglog.Kata Kunci: tungau; krepes; medium, jamur kupingAbstract: Wood ear mushrooms (Auricularia polythrica), that is commonly cultivated by mushroom farmers in the Special Region of Yogyakarta, is often plagued by a disease which has the local name : krepes. The group of mites (acarina) has been declared as the causal agent of krepes disease. Identification of the causes of crepes in Indonesian mushroom farming has not been carried out comprehensively. Identification of mites that cause krepes requires proper propagation techniques where the optimum growth medium serves as a basis for supporting mites culture techniques. This research aims to study the optimum medium for mite culture propagation. A variety of materials that are likely to be the preferred media for mites to grow were used, either on the invitro test or field test (mushroom baglog). Results showed that the mites prefer to live on the mushroom baglogs which has been covered by fungal mycelium and baglogs which has produced mushroom bodies. This growth preference is suggested to be related to the availability of nutrients from both mycelium and basidiocarp that is grown on the baglog..Keywords: mites; krepes; medium, wood ear mushroom
Efek Ekstrak Daun Bayam Brasil (Alternanthera sissoo hort) Terhadap Jumlah Limfosit dan Indeks Organ Timus dan Limpa Mencit Jantan Priskila Melinia Wuni; Kukuh Madyaningrana; Vinsa Cantya Prakasita
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p19

Abstract

Utilization of flora biodiversity is very potential to be explored as a source of medicine. Their phytochemicals content could be used as immunomodulator to help body's immunity fight pathogen. Brazilian spinach has the potential to be developed as an herbal-based immunostimulant because of its nutritional value and phytochemical compounds. This study aimed to study the effect of Brazilian spinach leaf extract on the immune response of male mice as shown by lymphocyte count and spleen and thymus index values. Extract of Brazilian spinach leaf was obtained by maceration method using 96% ethanol as solvent. Phytochemical were detected using qualitative biochemical methods and GC-MS. The in vivo test was carried out by giving 6 treatments to male mice orally in 7 days, namely aquades treatment, negative control (Na-CMC-aquadest), positive control (commercial immunostimulants), and three doses of Brazilian spinach leaf extract, namely 0.1563 mg/g. BB; 0.312 mg/g BW), and 0.468 mg/g BW. The number of lymphocytes was prepared and counted by blood smear, as well as the calculation of the spleen and thymus index based on fresh weight calculation. Qualitative biochemical assays showed identified flavonoids, alkaloids, saponins, tannins and steroids in Brazilian spinach leaf extract, meanwhile GC-MS assays showed 3 dominant compounds, namely neophytadiene, phytol and -Tocopheryl acetate. The administration of Brazilian spinach leaf extract with a dose of 0.1563 mg/g BW was the most effective in increasing lymphocytes count of tested animals. The thymus and spleen index values ??correlated with the increased number of lymphocytes in the administration of Brazilian spinach leaf extract. These results indicate the basic potential of using Brazilian spinach leaves as an immunostimulant.
THE EFFECT OF KESAMBI BARK EXTRACT ON MICE LYMPHOCYTE COUNT AND SPLEEN INDEX Christine Stefani Tamelan; Kukuh Madyaningrana; Vinsa Cantya Prakasita
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 8 No. 2 (2022): February 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.6039

Abstract

Kesambi is a plant widely used by people of Timor Island. Flavonoid content of Kesambi is potential to be used as immunomodulators. This study aimed  to study the effect of Kesambi bark extract on lymphocyte count and spleen index of male mice as an indicator of their immune responses. Research included identification of phytochemical compounds macerated from Kesambi bark, lymphocytes count and spleen index calculation. Mice injected with sheep erythrocytes were treated orally with three doses of Kesambi extract (0.113 mg/g BW, 0.491 mg/g BW, and 0.712 mg/g BW) for 10 days. Lymphocyte count using blood smear method was carried out on day 0, 5 and 10, while lymphocyte count using hematology analyzer and spleen index measurement were performed after mice sacrificed. Kesambi bark contains alkaloids, flavonoids, tannins, and steroids. Treatment with Kesambi bark extract reduced mice lymphocyte count. Administration of 0.491 mg/gr BW dose reduced the mice lymphocytes count close to the number of lymphocytes in the negative control group. The spleen index of mice had no correlation with changes in the number of lymphocytes. This preliminary study shows a potential use of Kesambi bark extract as a natural immunosuppressant.
Co-Authors -, Krismono Abrillian, Albert Allan Buddie Santoso Aniek Prasetyaningsih Ariestanti, Catarina Aprilia Aryasa, Natali Allya Graciandi Catarina Aprilia Ariestanti Christine Stefani Tamelan Djima, Elza Gitaa Eirene Djima Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Eunike Dian Swastoko Guruh Prihatmo Irene Agni Teatrawan Ite siga, Theresia Avilla Drira Kisworo Kisworo Kisworo, K Koniherawati Krismono Krismono Kristianto, Hizkia Andrian Kurnianto, Hizkia Andrian Limeranto, Desyana Millenia Meilani Apra Meilani Apra Mularum, Seta Nurhayati Nathanael, Michelle Aurelia Nugroho, Gregorius Delon Ajidamara Nurlia Widayanti Pirade, Emeralda Rey Prihatmo, Guruh Priskila Melinia Wuni Santoso, Julyana Dika Seta Nurhayati Mularum Setiadi, Claudine Greacia Sidauruk, Arkhey Sutanto, Haryati Bawole Vinsa Cantya Prakasita Widayanti, Nurlia