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All Journal Jurnal Info Kesehatan Laboratory Journal of Infectious Diseases
Agustina W. Djuma
Poltekkes Kemenkes Kupang
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology

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Uji Tuberkulosis Laten Pada Kontak Serumah Pasien BTA Positif Dengan Metode Mantoux Test Norma Tiku Kambuno; Yunita Helmina Senge; Agustina W. Djuma; Elisabeth N. Barung
JURNAL INFO KESEHATAN Vol 17 No 1 (2019): Jurnal Info Kesehatan
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol17.Iss1.239

Abstract

Background. Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Latent tuberculosis is one of the conditions that occur when exposed to TB bacteria but does not show clinical symptoms. Vulnerable family members infected with TB due to difficulty avoiding contact with patients. The tuberculin skin test is one of the tests used to diagnose latent TB. The purpose of this study was to determine the description of latent TB in-home contact with smear-positive TB patients. Determine the relationship between latent TB with age, gender, education, occupation, and smoking status. Method. The type of research is correlation analytic research with a cross-sectional design with a total sample of 22 people. Results. Based on the results of the study as many as 15 people (68.2%) with induration > 10 mm, and 7 people (31, 8%) with induration <10 mm. While based on the results of statistical tests to see the relationship between the influence of age, sex, education level, occupation and smoking status with latent TB status obtained approx significant> 0.05. Conclusion. Thus the prevalence of latent TB at home contact with positive smear patients was 68.2% and there was no relationship between age, sex, education level, occupation and smoking status with TB status.
Utilization of Teak Leaf Extract as a Substitute for Eosin in Hematoxylin Eosin Staining Agnes Desiana Paa; Agustina W. Djuma; Juliana Christyaningsih
Laboratory Journal of Infectious Diseases Vol. 2 No. 1 (2026): Laboratory Journal of Infectious Diseases
Publisher : Poltekkes Kemenkes Kupang

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Abstract

Hematoxylin–Eosin (HE) staining is widely used in histopathology to visualize tissue morphology. Eosin, a synthetic dye commonly used to stain cytoplasmic components, may cause environmental and health concerns, encouraging the search for safer natural alternatives. Young teak leaves (Tectona grandis) contain anthocyanin pigments with potential as natural staining agents. This study evaluated the effectiveness of teak leaf extract as a substitute for eosin in HE staining of uterine tissue. A quasi-experimental post-test only control group design was applied. Young teak leaves were extracted through maceration using 95% ethanol for seven days, followed by filtration and evaporation to obtain a concentrated extract. Uterine tissue preparations were stained using hematoxylin combined with 75% teak leaf extract, while eosin staining served as the control. Microscopic evaluation at 400x magnification was performed by an anatomical pathology specialist using a staining quality scoring system. The control group stained with eosin demonstrated optimal staining quality, with clear bluish-purple nuclei and bright pink cytoplasm, achieving a good score in all slides. In comparison, teak leaf extract produced pale purple nuclei and brownish-pink cytoplasm that remained distinguishable morphologically, though with lower staining quality. Overall, teak leaf extract demonstrated potential as a natural alternative to eosin, but further optimization is needed to improve staining performance.
Co-Authors Agnes Desiana Paa Elisabeth N. Barung Juliana Christyaningsih Norma Tiku Kambuno Yunita Helmina Senge