P.K. Tahuk
The Agriculture Faculty of Timor University-East Nusa Tenggara. Jl . Mayjend El Tari, Kefamenanu, Center of North Timor Regency-85613

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Journal : Journal of Tropical Animal Science and Technology

The Effect of Using Different Levels of Thinner Olive Oil (Extra Virgin Olive Oil) on the Viability and Abnormality of Spermatozoa and pH of the Semen Duroc Pigs Stefanus Nahak; Agustinus Agung Dethan; Paulus Klau Tahuk
Journal of Tropical Animal Science and Technology Vol 3 No 2 (2021): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v3i2.1024

Abstract

This study aims to determine the effect of using different levels of thinner olive oil (extra virgin olive oil) on the viability and abnormality of spermatozoa and the pH of the semen of duroc pigs. The research took place in September 2020 in Noenebu, Tapenpah Village, Insana District, North Central Timor Regency, and the Laboratory of the Faculty of Agriculture, University of Timor. This study used a completely randomized design (CRD) with 4 treatments and 5 replications. Treatment R1 consist of 10% tris solution, 10% olive oil, 80% semen; R2 treatment consist of 15% tris solution, 15% olive oil, 70% semen; R3 treatment consist of 20% tris solution, 20% olive oil, 60% semen, and R4 treatment consist of 25% tris solution, 25% olive oil, 50% semen. The results showed that the effect of different treatments was very significant (P <0.01) on the viability of spermatozoa. The mean spermatozoa viability of each treatment was R1 of 89 ± 2.57%, R2 of 76.9 ± 12.51%, R3 of 62.8 ± 3.11%, and R4 treatment of 41.2 ± 15, 38%. The spermatozoa abnormalities were significantly different (P <0.05). Where the abnormality of the spermatozoa in treatment R1 was 8.8 ± 1.30%, R2 was 10.4 ± 1.67%, R3 was 11.6 ± 1.81%, and R4 was 14.4 ± 3.78%. The degree of acidity (pH) of the semen was not significantly different between treatments. The pH value of each treatment was R1 of 7.92 ± 0.83, R2 of 8.04 ± 0.54, R3 of 7.96 ± 0.39; and R4 treatment of 8.06 ± 0.64. It can be concluded that the addition of 10% to 20% olive oil can maintain the viability and abnormality of duroc boar male spermatozoa. However, the use of olive oil up to 20% does not affect to degree of acidity of the semen produced.
Quality of Landrace Semen in Yolk Citrate Cement which Plus Glucose with Different Concentrations Albertus Baku; Agustinus A Dethan; Paulus Klau Tahuk
Journal of Tropical Animal Science and Technology Vol 4 No 1 (2022): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v4i1.1268

Abstract

The purpose of this study was to determine the effect of using yolk citrate diluent with different concentrations of glucose added on the motility, viability, abnormalities and pH of landrace pig cement.The cement used was fresh cement from a 3 year old Landrace pig stud.This study used an experimental method using a completely randomized design (CRD) with four treatments (P1 5%, P2 10%, P3 15% and P4 20%) and four replications so that there were 16 experimental units.The results showed that the concentration of glucose in the yolk citrate diluent was not significantly different (P>0.05) on the motility of spermatozoa with the average value of each treatment being P1 (65%), P2 (60%), P3 (63, 8%) and P4 (67.5%).The percentage of spermatozoa viability was very significantly different (P<0.01) between treatments, where in treatment P2 was relatively the same as treatment P3 and P4 but higher than treatment P1 with the value of each treatment P1 (83%), P2 (90.1 %), P3 (93.5%) and P4 (91.5%). Percentage of spermatozoa abnormalities resulting from analysis of variance (ANOVA) from each treatment there was a non-significant difference (P>0.05) where the values ​​were P1 (8.2%), P2 (9.2%), P3 (8.1%) and P4 (9.2%). The pH was significantly different (P<0.05) between treatments, where P1 treatment was relatively the same as P2 but higher than P4 treatment. On the other hand, treatment P3 was relatively the same as treatment P1, P2 and P4, with the values ​​of each treatment being P1 (8.36), P2 (7.99), P3 (7.676) and P4 (6.83). It can be concluded that the use of different glucose concentrations in the egg yolk citrate diluent with good quality, namely in the P3 treatment with a glucose concentration of 15%, the average percentage of individual motility was 64%, the percentage of live spermatozoa was 93.5%, the percentage of abnormal spermatozoa was 8.1%. and the average pH of cement is 7.68. It was concluded that the quality semen of Landrace pig could be improved optimally using glucose concentration in egg yolk citrate diluent by 15%.
The Effect Use of Different Binding Materials on Water Holding Capacity, Water Content and Crude Fiber Content of Chicken Nuggets Alfonsus Liquori Taus; Paulus Klau Tahuk; Kristoforus W Kia
Journal of Tropical Animal Science and Technology Vol 4 No 1 (2022): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v4i1.1330

Abstract

This study aims to determine the water holding capacity, moisture content and crude fiber content of chicken nuggets made using different types of flour as a binder. This research was conducted at the Laboratory of the Faculty of Animal Husbandry, Nusa Cendana University, Kupang for 1 week. The method used in this study was a completely randomized design (CRD) with 3 treatments and 3 replications. The treatments were P1 (making nuggets with porang flour), P2 (making nuggets with tapioca flour), and P3 (making nuggets with wheat flour). The variables observed in this study included water holding capacity, water content and crude fiber content. The data obtained were analyzed using the Anova test and Duncan's test. The results of the study showed that the water holding capacity of chicken nuggets P1, P2, P3 were 36.956%, 34.621%, 32.756%, respectively. The water content of chicken nuggets was 64.17%, 59.95%, 57.71%, respectively. The crude fiber content of chicken nuggets is 1.387%, 1.169%, and 0.638% respectively. The statistical analysis showed that the treatment had a significant effect on the water holding capacity of chicken nuggets, water content of chicken nuggets and crude fiber content of chicken nuggets (P<0.05). It was concluded that the water content of chicken nuggets made from wheat flour and tapioca was in accordance with SNI, while the production of nuggets made from porang flour exceeded the Indonesian National Standard, but porang flour had the advantage of being able to bind water better.