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All Journal Jurnal Ilmu Kimia Universitas Brawijaya
Elvanila Tanjung
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Chemical Engineering, Chemistry & Bioengineering Chemistry

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AMOBILISASI PEKTINASE HASIL ISOLASI DARI Aspergillus niger MENGGUNAKAN MATRIKS KARAGENAN Tanjung, Elvanila; Roosdiana, Anna; Mardiana, Diah
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

ABSTRACT Pectinase isolated from Aspergillus niger was immobilized by entrapping method in a matrix of carrageenan. The aim of this research was to determine the optimum concentration of carrageenan and enzyme. Carrageenan concentration varied (0.017; 0.033; 0.050; 0.067; 0.083) g/mL, while the concentration of enzyme  varied  (0.632; 1.263; 1.895; 2.526; 3.158) mg/mL. Protein content was determined by Biuret reagent  and enzyme activity was determined from the number of reduced sugar as a result of  hydrolyzed pectin per minute. The results showed that optimum concentration of carrageenan was 0.050 g/mL and the optimum enzyme concentration was 1.263 mg/mL with the amount of entrapping enzyme was 3.048 mg/g carrageenan and maximum activity of  348.5 units. Key words: activity, Aspergillus niger, carrageenan, pectinase.
Co-Authors Anna Roosdiana Diah Mardiana