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All Journal Jurnal Penelitian Saintek Jurnal Ilmu Kimia Universitas Brawijaya Natural B The Journal of Pure and Applied Chemistry Research Science and Technology Indonesia ALCHEMY Jurnal Penelitian Kimia Chemistry Indonesian Journal of Chemical Research JKPK (Jurnal Kimia dan Pendidikan Kimia) Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Molekul: Jurnal Ilmiah Kimia
Diah Mardiana
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Brawijaya, Jl Veteran Malang 65145, Jawa Timur, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Energy Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Physics

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AMOBILISASI PEKTINASE DARI Bacillus firmus MENGGUNAKAN MATRIKS OPP (OXIDIZED POLYPROPYLENE)-KITOSAN Sari, Ayunda Arum; Roosdiana, Anna; Mardiana, Diah
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

A Pectinase is an enzyme that can hydrolyze pectin compounds into galacturonic acid. In order to enhance the pectinase efficiency, the enzyme can be immobilized in certain matrix. The pectinase was isolated from Bacillus firmus, and then purified with ammonium sulphate with 20-60% saturated level, followed by dialysis. The aims of the research were to determine the optimum conditions of immobilized pectinase by physical adsorption on oxidized polypropylene (OPP) coated chitosan, which included the shaking time and enzyme concentration. Initial protein used for immobilizing free pectinase was 1.367 mg/mL and the activity was 241.1 units. The results showed that the optimum condition of pectinase immobilization was achieved on shaking time of 3 hours and pectinase concentration of 1.094 mg/mL with amount of pectinase adsorbed at 53,98 mg/g within activity of 220.2 units.
KINETIKA REAKSI FERMENTASI GLUKOSA HASIL HIDROLISIS PATI BIJI DURIAN MENJADI ETANOL Salsabila, Usyqi; Mardiana, Diah; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Ethanol can be produced by glucose fermentation process using Saccharomyces cerevisiae. The potential source of glucose is starch of durian seed. This research studies about determine the kinetics model of ethanol fermentation of glucose and to identify the physical properties of ethanol produced. Glucose fermentation using Saccharomyces cerevisiae for 72 hours at various initial concentrations of 20, 40, 60 and 80 ppm has been conducted. After ethanol produced was reacted with potassium dichromate using Conway crucible. It was then analyzed by UV-Vis spectrophotometer at 605 nm. The physical properties of ethanol determined were density and refraction index. The result showed that the kinetics model of ethanol fermentation obtained was accordance with  = -0,0011  + 0,0002 equation. As for Michaelis-Menten (Km) constant was 5,5 ppm-1 and maximum velocity (Vmax) 5000 ppm.hour-1. In addition, its physical properties i.e. density and refractive index were in the range of 0,9767-0,9809 g/mL and 1,3347-1,3365 respectively.
AMOBILISASI PEKTINASE HASIL ISOLASI DARI Aspergillus niger MENGGUNAKAN MATRIKS KARAGENAN Tanjung, Elvanila; Roosdiana, Anna; Mardiana, Diah
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

ABSTRACT Pectinase isolated from Aspergillus niger was immobilized by entrapping method in a matrix of carrageenan. The aim of this research was to determine the optimum concentration of carrageenan and enzyme. Carrageenan concentration varied (0.017; 0.033; 0.050; 0.067; 0.083) g/mL, while the concentration of enzyme  varied  (0.632; 1.263; 1.895; 2.526; 3.158) mg/mL. Protein content was determined by Biuret reagent  and enzyme activity was determined from the number of reduced sugar as a result of  hydrolyzed pectin per minute. The results showed that optimum concentration of carrageenan was 0.050 g/mL and the optimum enzyme concentration was 1.263 mg/mL with the amount of entrapping enzyme was 3.048 mg/g carrageenan and maximum activity of  348.5 units. Key words: activity, Aspergillus niger, carrageenan, pectinase.
PEMURNIAN ETANOL SECARA MIKROFILTRASI MENGGUNAKAN MEMBRAN SELULOSA ESTER Pratama, Agung Yanuar; Mardiana, Diah; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Studi tentang pemurnian etanol secara mikrofiltrasi menggunakan membran selulosa ester telah dilakukan. Tujuan penelitian ini adalah untuk mengetahui apakah metode mikrofiltrasi dapat digunakan untuk meningkatkan kemurnian etanol, dan mengetahui pengaruh waktu filtrasi terhadap tingkat kemurnian etanol. Membran yang digunakan adalah mix-selulosa ester yang telah dikompaksi dengan sampel yang digunakan etanol 96%. Karakter membran yang digunakan dievaluasi berdasarkan hasil SEM dan spektrofotometri FT-IR.  Mikrofiltrasi dilakukan hingga 120 menit pada tekanan 1 atm. Analisis hasil filtrasi didasarkan pada massa jenis dan indeks bias. Hasil penelitian menunjukkan bahwa waktu filtrasi berpengaruh terhadap kemampuan membran menahan etanol, meskipun membran selulosa ester kurang sesuai untuk meningkatkan kemurnian etanol secara mikrofiltrasi.   Kata Kunci : Etanol, Mikrofiltrasi, Selulosa Ester
PENGARUH HIDROFILISITAS MEMBRAN TERHADAP PENINGKATAN KEMURNIAN ETANOL SECARA DESTILASI Sitanggang, Fetty Alvionita; Mardiana, Diah; Indahyanti, Ellya
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 2 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Metoda destilasi membran dimungkinkan digunakan untuk meningkatkan kemurnian etanol. Dua jenis membran yang digunakan pada kajian ini adalah membran selulosa ester dan membran poliamida. Tujuan penelitian ini adalah untuk mengetahui pengaruh hidrofilisitas membran dan waktu destilasi terhadap kemurnian etanol. Destilasi yang dikombinasikan dengan membran dilakukan pada temperatur 80oC dengan variasi waktu destilasi 1 sampai 3 jam. Hasil destilasi, baik destilat maupun residu, dianalisis berdasarkan  nilai indeks bias dan massa jenisnya. Sebagai pendukung juga telah dilakukan analisis sifat termal dan derajat swelling membran. Hasil analisis termal menunjukkan bahwa membran selulosa ester tidak sesuai untuk pemurnian secara destilasi, Membran poliamida yang lebih bersifat hidrofilik dibandingkan membran selulosa ester lebih mampu menahan air, meskipun belum dapat digunakan untuk meningkatkan kemurnian etanol secara destilasi. Semakin lama waktu destilasi, kemampuan membran poliamida untuk melewatkan etanol semakin tinggi.Kata kunci: etanol, destilasi, membran, hidrofilik
PEMANFAATAN NATA DE COCO TERMODIFIKASI ASAM SITRAT SEBAGAI BAHAN BKU MEMBRAN Syaifudin, Achmad; Kamulyan, Budi; Mardiana, Diah
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 1 (2015)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

In order to discover the alternatif of membrane raw material, modification of nata de coco by esterification has been conducted. In this research, it has been done using citric acid and dibutyltin oxide catalyst. This research aims was to determine the effect of esterification on the chemical structure, thermal properties and hydrophilicity of modified cellulose. Reaction of solid state system using closed reactor supporting by mechanical strirer has been done for 3 hours at 155oC. It was used for reacting 5 g of cellulose and 0.3 g of dibutyltin oxide with adding of citric acid were 5, 15, 25, 35 and 45 g. Product characterization were investigated by FTIR spectrophotometry for predicting the chemical structure and thermal properties using DTA. Where as, hydrophilicity was determined by its swelling index of aquades and ethanol up to 6 hours. The result of FTIR spectrum showed that ester cellulose has been produced, indicating by specific peak of carbonyl group at wave number 1733.89 cm-1. DTA thermogram of ester cellulose showed a glass transition at 7.6oC and endothermic peak at 27.6 and 70.5°C, respectively.   In addition, swelling equilibrium of 3 hours has been obtained with swelling index for aquadest and ethanol were 0.209 and 0.361 time, respectively. Key words:citric acid, esterification, nata de coco
Early Poly Mass Effect (trimethylene-sebasat) On Biodegradation Rate In Liquid Media Using Mucor Miehei In Aerobic Kholisul Fuad, Akhmad; Mardiana, Diah; Roosdiana, Anna
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.176 KB) | DOI: 10.21776/ub.natural-b.2012.001.03.10

Abstract

The author has conducted research about the effect of poly(trimethylene-sebacate) mass toward the rate of biodegradation using Mucor miehei in aerobic liquid media. Poly(trimethylene-sebacate) is a biodegradable linear aliphatic polyester, that can be degraded by Mucor miehei lipase. To determine the effect of poly(trimethylene-sebacate) mass in the biodegradation, the mass of poly(trimethylene-sebacate) were varied 0.06 g, 0.08 g, 0.1 g, 0.12 g and 0.14 g. Biodegradation process carried out for 12 hours, using liquid of Complex media, which was nutrient rich for Mucor miehei growth, and solution at pH 5. The resulting CO2 gas was flowed into the 50 mL reservoir of 0.1 M NaOH, followed by titration using 0.05 M HCl and MO (methyl orange) and PP (phenolptalein) indicator. The rate of CO2 gas were 0.287 x 10-3 M/h; 0.102 x 10-3 M/h; 0.137 x 10-3 M/h; 0.016 x 10-3 M/h; and 0.039 x 10-3 M/h respectively. The greater rate of CO2 produced the lower mass of poly(trimethylene-sebacate).
Adsorption of Mg (II) Ion Using Chlorophilized Chitin Adsorbent Darjito Darjito; Rachmat Triandi; Diah Mardiana; M. Fathoni
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.582 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.1

Abstract

A study concerning the adsorption of Mg (II) ions using phosporilation chitin adsorbent. The process is a surface modification of adsorbent chitin by enriching the phosphate group that acts as a hard base so it would be more suitable binding Mg (II) ions, which acts as a hard acid. This research aims to study the effect of contact time and concentration variations of Mg (II) ions on the adsorption process using phosporilation chitin adsorbent. Variables to be examined in this study in order are contact times (40, 60, 80, 100, and 120 minutes) and the concentration of Mg (II) ions variation are (10, 25, 50, 75, 100, 125, 150, 175, and 200 ppm). The results for the characterization of phosporilation chitin adsorbent can be detect from the IR absorption band at wavenumber 1099.35 cm-1 are suspected of phosphate ions and IR absorption band at wave number 1272.93 cm-1 and 1201.57 cm-1 are estimated as phosphate covalent. The contacts times significantly affect the adsorption Mg (II) ions by the phosporilation chitin adsorbent, then the optimum of contact time is 120 minutes. Adsorption capacity of phosporilation chitin adsorbent for the Mg(II) ions is 499 mg/g.
Effect of Adding Ions - Metal Ions to Pectinase Activity Isolation from Bacillus Subtilis on Bleaching Paper Mardiana Prasetyani Putri; Anna Roosdiana; Sasangka Prasetyawan; Diah Mardiana
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.888 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.11

Abstract

Pectinase enzyme used as biocatalyst to overhaul pectat compounds or pectin. Pectinase avtivity was affected by metal ions. The purpose of this study is to determine the effect of adding metal ions on pectinase activity and its inhibition type.. Determination of enzyme activity was based on galacturonic acid produced, which was analyzed by spectrophotometric using DNS reagent. Concentration of metal ions used i.e. Zn2+, Cu2+, Fe3+ were 2-10 mM. The result showed that Vm and KM number without ions were 161.29 µmol/mL minutes and 0.55%(w/v), respectively. While the presence of ions Zn2+, Cu2+, Fe3+ were producing Vmapp 147.06; 156.25 and 153.85µg.ml-1min-1, KMapp of 0.56%; 0.63% and 0.65%, and also KI of 333,33; 41,38; 32,9. The inhibition to metal ions are non-competitif inhibition.
Effect of Metal Ions on Pectinase Activity from Aspergillus niger on Purifying Guava Fruit Dian Puspita Anggraini; Anna Roosdiana; Sasangka Prasetyawan; Diah Mardiana
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.749 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.10

Abstract

Pectinase can be used on various fruit juices clarification; however, the activities can be affected by their metal ions content. The purpose of this study was to determine the type of metal ions inhibition i.e. Zn2+; Mg2+ and Ca2+ on the pectinase activity of Aspergillus niger, and its potential application in guava juice clarification. Activity assays performed at pH 5, temperature 50°C during 55minutes and each ion concentration were 2-10 mM. Galacturonic acid concentration, as the product of pectin substrate hydrolysis, used as the basis for determining its activity and analyzed by visible spectrophotometry. The results showed that the ions concentration of Zn2+ and Mg2+ more than 4 mM were inhibitor for pectinase activity while Ca2+ ion concentration was 6mM. KI values for the metal ions Zn2+; Mg2+ and Ca2+ at concentrations of 6mM were 176.99; 326.09 and 280.37 mM respectively. Pectinase from Aspergillus niger were able to reduce 18% of pectin of guava juice.  
Co-Authors Agung Yanuar Pratama Akhmad Kholisul Fuad Ani Mulyasuryani Anna Roosdiana Anna Roosdiana Arie Srihardyastutie Ayunda Arum Sari Bambang Poerwadi Barlah Rumhayati Budi Kamulyan Budi Kamulyan Darjito Darjito Dewi Umaningrum Dian Puspita Anggraini Dwi Siswanta El Fajriyah Aulia Putri Ellya Indahyanti Elvanila Tanjung Fetty Alvionita Sitanggang Hasanuddin Hasanuddin Jumina Jumina Khoirul Ngibad Layta Dinira Lukman Hakim M. Fathoni Mardhatillah Mardhatillah Mardiana Prasetyani Putri Maria Dewi Astuti Maria L.A.D Lestari Maria Lucia A.D Lestari Maria Lucia Ardhani Dwi Lestari Rachmat Triandi Radna Nurmasari Riawati Riawati Ridho Arief Al Rasyid Ridho Firmansyah Sasangka Prasetyawan Sepriana Puspita Dewi Syaiful Siannita Chandra Syaifudin, Achmad Ulfa Andayani Ulfa Andayani, Ulfa Urnik Rokhiyah Usyqi Salsabila Zubaidah Ningsih Zubaidah Ningsih Zubaidah Ningsih Zubaidah Ningsih