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All Journal Indonesian Journal of Biotechnology JKPK (Jurnal Kimia dan Pendidikan Kimia)
Arief Taslihan
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology

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Genetic Heterogenity Profile of Penaeus monodon Broodstock F1 Revealed by Mitochondria DNA-RFLP and RAPD Prastowo, Bambang Widyo; Rahardianti, Rahayu; Nur, Evi Maftuti; Taslihan, Arief
Indonesian Journal of Biotechnology Vol 12, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.63 KB)

Abstract

The genetic heterogeneity of Penaeus monodon broodstock F1 was evaluated using Restriction Fragment LengthPolymorphism (RFLP-mtDNA) and Random Amplified Polymorphic DNA (RAPD) analysis. The RFLP analysis wasconducted by amplifying 16SrDNA region and digested with restriction enzyme Nde II. According to the RFLPanalysis, heterogeneity value of P. monodon F1 broodstock population is 0,0422; male F1 population is 0,0613 andfemale F1 population is 0,1252. The primer OPA2 was used in RAPD analysis. According to the RAPD analysis,heterogeneity value of P. monodon F1 broodstock population is 0,0417; male F1 population is 0,0653 and female F1population is 0,1104. The results of this research showed that either RFLP or RAPD can be used as a family specificmarker for Penaeus monodon.Key words : Penaeus monodon, RFLP, RAPD, heterogeneity, genetic marker
Laminarin crude extract characterization of Sargassum sp. originated from Jepara-Indonesia with Laminarin Acid Extraction method using acetic acid solvent Sari, Ratna Indria; Suryati, Suryati; Amalia, Indrian Rizka; Taslihan, Arief
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 8, No 1 (2023): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v8i1.72313

Abstract

Laminarin is a bioactive compound found in Sargassum sp. whose existence is widespread in Indonesia, including in Jepara, Central Java, Indonesia. Laminarin can be used in food, pharmaceutical, cosmetic, and feed industries as it is beneficial as an anti-inflammatory, antioxidant and regulates gut microbiota. This study aimed to extract laminarin from Sargassum sp. that originated from Jepara-Indonesia by laminarin acid extraction procedure with an acetic acid solvent (CH3COOH) which was safer, cheaper and easy to obtain. Characterization of laminarin crude extract use Fourier Transform Infrared (FT-IR); meanwhile, nutrition tested are moisture, ash, crude protein and crude fiber content. The results showed that laminarin extraction of Sargassum sp. from Jepara using acetic acid solvent (L-ACT) obtained the highest yield of 15.5% with a solvent ratio of 1:5 (w/v) but still lower than the yield extraction using hydrochloric acid (L-HCl) with a solvent ratio of 1:10 (w/v) that was 24.17%. FT-IR absorption band at 2359.96 cm-1 (-OH bending; transmission angle peak), 1538.54 cm-1 (C-C aromatic; carboxyl groups), 1409.35 cm-1 (C-H bending; carboxyl groups), 1230.89 cm-1 (C-O-C stretching; sugar region), 1022.58 cm-1 (R-O-R (acetal)), and at 944.94 cm-1 (O-R cyclic) of the L-ACT samples were similar to those of the L-HCl and standard laminarin references in other studies that used as a positive control, confirming the presence of laminarin. The L-ACT extract had lower ash, crude protein content and higher water content compared to L-HCl crude extract. Meanwhile, the crude fiber content in both L-ACT and L-HCL was not identified. The results showed that Sargassum sp. from Jepara was the potential source of laminarin for many industries (pharmacy, cosmetic, food), and acetic acid could be used as a suitable solvent to extract laminarin in the LAE method. 
Co-Authors Amalia, Indrian Rizka Bambang Widyo Prastowo Evi Maftuti Nur Rahayu Rahardianti Sari, Ratna Indria Suryati Suryati