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Amalia, Indrian Rizka
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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The Growth Test of Marine Microalgae Porphyridium cruentum in Different Salinity on Small Scale Culture Amalia, Indrian Rizka; Nur, Abidin; Ruliaty, Lisa; Sari, Ratna Indria
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.5301

Abstract

This study aimed to know the growth response and proximate value of Porphyridium cruentum cultured in different salinities of 30 ppt, 15 ppt and 0 ppt. Culture media of 30 ppt and 15 ppt salinity were prepared with sterile seawater, while 0 ppt was from sterile aquades. Fertilizer used was KW21 (Daiichi Seimo Co., Ltd., Kumamoto, Japan) with a dose of 1 ml.l-1. All treatments were cultured for 11 days or until the cell reached exponential-stationer phase, then being growth observed and proximate analyzed. The result showed that all treatments gave the same growth pattern. Control 30 ppt had the highest cell density compare to treatments 0 ppt and 15 ppt, while 0 ppt had higher cell density to 15 ppt. The result of ANOVA (95% of significancy) showed that there was no significant different of cell density with value of 0.232. Proximate analysis showed that all treatments had high water content above 95%. The highest value of energy, carbohydrates and crude fiber were found in 0 ppt treatment, while the highest value of protein and ash were found in 30 ppt. The result from ANOVA showed that there was significant difference in proximate value with significancy less than 0.05
Low Temperature Preservation on Dense Biomass of Nannochloropsis oculata Amalia, Indrian Rizka; Nur, Abidin
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9633

Abstract

The consistency supply of microalgae is crucial for hatchery as it plays important role as feed for larvae in the early stages. Thus, ensuring the availability of microalgae needs efficient management of feed, including the technique of biomass preservation. The purpose of this research was to observe the application of various low temperature to preserve Nannochloropsis oculata as feed. The source of N.oculata was filtered through a double set of 0.45 micron of cartridge which then preserved based on treatments A (5°C); B (-20° C); and C (15°C) for 30 days. After 30 days of preservation, each treatment as inoculum were cultured in the volume of 2 liter. Initial density of N.oculata was 23-24x106 cells.ml-1 placed into medium consisted of sterile sea water salinity 30 ppm, KW21™ fertilizer dose 1 ml.l-1. Culture environment was set with illumination 3.000-4.000 lux, homogenized with constant aeration, with 15 days of culture period. The result showed that on the days 9, cell density on treatment A, B and C were significantly different each other (sig 0.001), where treatment A had the highest cell density at 57.42±6.95x106 cell.ml-1, then followed with treatment C with 38.33±2.08x106 cell.ml-1 and B with 20.33±5.35x106 cell.ml-1. The use of low temperature as preservation for N.oculata could help low-middle hatchery with remoted area to maintain the sustainability of N.oculata stocks.
The Effect of Dietary Probiotic and Oxygen Supply on the Growth and Survival Rate of Eels (Anguilla bicollor) Garnawansah, Gagan; Permana, Dian; Hasbullah, Dasep; Siregar, Zaenal Arifin; Wahyudi, Dzikri; Handoyo, Boyun; Amalia, Indrian Rizka; Sari, Ratna Indria
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9998

Abstract

The aquaculture of Anguilla bicollor has significant economic potential, but persistent challenges continue to hinder profitability for farmers. Two of the most pressing issues are low dissolved oxygen levels and poor feed conversion efficiency. These problems slow eel growth and drive-up operational costs, creating a bottleneck for sustainable production. This study examines a combined strategy to tackle these challenges by integrating probiotics (Lactobacillus sp.) and paddlewheel aeration. Research was conducted at BLUPPB Karawang, using 600 juvenile eels in ponds subjected to four distinct treatments: (1) probiotics and paddlewheels together, (2) paddlewheels-only, (3) probiotics-only, and (4) a control group with no intervention. Over a 45-day period, survival rates (SR), relative growth rates (RGR), and feed conversion ratios (FCR) were carefully monitored. The findings were promising. Ponds that used both probiotics and paddlewheels achieved the best results: an RGR of 1.19%, an SR of 100%, and an FCR of 1.09. Probiotics contributed to better gut health and digestion, while the paddlewheels boosted water oxygenation—creating an ideal growth environment. While no direct interaction between the two methods was observed, their combined application proved highly effective in improving productivity and sustainability. This integrated approach offers a practical solution to some of the most significant challenges in Anguilla bicollor aquaculture.
Co-Authors Boyun Handoyo Dian Permana, Dian Garnawansah, Gagan Hasbullah, Dasep Nur, Abidin Ruliaty, Lisa Sari, Ratna Indria Siregar, Zaenal Arifin Wahyudi, Dzikri