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All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA JURNAL AGRONIDA
Unun Triasih
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Agriculture, Biological Sciences & Forestry

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Test of Various Carrier Materials Against Viability and Conidia Density in Some Liquid Biopesticides of Entomopathogenic Fungi Unun Triasih; dina agustina; mutia erti dwiastuti; susi wuryantini
JURNAL AGRONIDA Vol. 5 No. 1 (2019)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1252.292 KB) | DOI: 10.30997/jag.v5i1.1851

Abstract

Indonesia is a country that has a large biological diversity but has not been fully utilized for agriculture. The effectiveness of biological agents in controlling pests and plant diseases still needs further investigation, one of which is the use of entomopathogenic fungi as biopesticides. The purpose of this study was to determine the effect of storage on the viability and density of conidia in several entomopathogenic fungi; Beauveria bassiana, Metarhizium anisopliae, Paecilomyces. This study used 5 entomopathogenic fungi isolates, 2 Beauveria bassiana isolates (JBG and JBR), 2 Metarhizium anisopliae isolates (JBG and UST), 1 Paecilomyces. Biopesticide carrier material consisted of 6 carrier materials, namely soybean decoction waste, soy washing waste, Potato Dextrose Agar, tapioca waste, rice waste, rice washing waste and coconut water. Biopesticides have been made one year before and the viability and complexity of the conidia is re-examined one year later. From the observations of conidial density, the best was found in Beauveria bassiana JBG liquid biopesticide of 4.8 x 10 x conidia / ml from the initial density of 3.4 x 10⁸ / ml in the soybean decoction (RK) carrier material. The highest viability was found in JBG Metharizium anisopliae on soybean washing waste material by 14%. Key words: Conidia viability, conidial density, entomopathogenic fungi
Potential of Antagonistic Fungi in Inhibiting the Growth of Botryodiplodia theobromae Fungi Causes Stem Rot Disease in Citrus Dina Agustina; unun triasih; mutia erti dwiastuti; rudi cahyo wicaksono
JURNAL AGRONIDA Vol. 5 No. 1 (2019)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1193.359 KB) | DOI: 10.30997/jag.v5i1.1852

Abstract

Soil microorganisms such as Trichoderma asperellum and Gliocladium sp have the potential to become biological control agents in inhibiting the growth of fungi Botryodiplodia theobrome which causes stem rot disease in citrus plants. The purpose of this study was to determine the potential of T.asperellum and Gliocladium sp fungi in inhibiting the growth of B. theobrome fungus causing stem end rot disease in citrus plants. The antagonistic test was carried out using the dual culture method using PDA media; observations were made on the growth of pathogenic fungi and antagonistic fungi to measure their inhibitory power. The results of the observation and calculation of the fungal growth rate were analyzed using the T test with a level of 5%. The pathogen fungal growth rate in the antagonistic test was slower than control and inhibition of B. theobrome by T.asperellum and Gliocladium sp were respectively at 78.67% and 84.56%. The mechanism of the two antagonistic fungi has the nature of competition and parasitism because of the broader inhibition spectrum than the pathogenic fungi. Keywords: Botryodiplodia theobromae, Trichoderma asperellum, Gliocladium sp, antagonistic test.
Morphological and molecular identification as well pathogenicity of the causal agents of fruit rot disease of apple manalagi (Malus sylvestris) in Pujon, East Java Unun Triasih; Abadi, Abdul Latief; Muhibbudin, Anton; Widyaningsih, Sri
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12356-66

Abstract

Apple fruit rot disease is one of the major apple diseases in Indonesia. It has been caused by several species of pathogenic within the genus Colletotrichum. This research aims to identify species of the pathogen causing apple fruit rot disease in Pujon, East Java. Suspected fungal isolates were identified based on morphological (macroscopic and microscopic) and molecular characteristics. Based on the morphological observation, five isolates were identified as Colletotrichum spp. These isolates have similar morphological characteristics such as white-greyish colony color, texture colony velvety, zonation conidia concentric, round cylindrical conidial end, and conidia with 10.4–12.8 ?m in length and width 3.1–3.52 ?m, respectively. Isolates M1 showed the highest pathogenicity, therefore selected for molecular identification. Molecular identification was conducted using ITS1 and ITS4 primer, AM1 was identified as C. gloeosporioides with 99.57% similarity to C. gloeosporioides JX-19 variety from China.
Co-Authors Abdul Latief Abadi Anton Muhibbudin Dina Agustina Mutia Erti Dwiastuti Rudi Cahyo Wicaksono Sri Widyaningsih Susi Wuryantini