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Aktivitas Antibakteri Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus Resistensi Antibiotik Rante, Herlina; Nasriah, Nasriah; Tayeb, Rosany
Jurnal Pharmascience Vol 11, No 1 (2024): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i1.16841

Abstract

Masalah kesehatan yang berhubungan dengan infeksi merupakan tantangan yang dihadapi tidak hanya oleh negara-negara maju tetapi juga negara-negara berkembang. Meningkatnya jumlah kasus bakteri yang kebal terhadap antibiotik telah mendorong usaha untuk menemukan sumber-sumber antibakteri dari bahan alami. Salah satu tumbuhan yang diduga memiliki kemampuan untuk melawan bakteri Staphylococcus aureus dan Escherichia coli adalah tanaman belimbing wuluh. Penelitian ini bertujuan untuk menguji efek antibakteri dari ekstrak etanol daun belimbing wuluh terhadap S.aureus dan E.coli resisten antibiotik. Ekstraksi dilakukan pada daun belimbing wuluh menggunakan etanol 70% dengan metode maserasi. Setelah itu, ekstrak etanol dibuat dalam seri konsentrasi 1,25%, 2,5%, 5%, dan 10% b/v. Uji aktivitas antibakteri dilakukan melalui metode difusi agar pada medium Mueller Hinton Agar dengan bakteri uji Escherichia coli dan Staphylococcus aureus yang telah resisten terhadap antibiotik. Hasil penelitian  menunjukkan bahwa ekstrak etanol dari daun belimbing wuluh ( A. bilimbi L) efektif menghambat pertumbuhan bakteri uji pada konsentrasi sebesar 1,25% (setara dengan 0,25 mg/paper disk). Kata Kunci: Bakteri Resisten, Maserasi, Difusi Agar, Mueller Hinton Agar, Pertumbuhan Bakteri Health issues related to infections pose a challenge not only to developed countries but also to developing nations. The increasing number of antibiotic-resistant bacterial cases has driven efforts to discover natural sources of antibacterial agents. One plant suspected to possess the ability to combat Staphylococcus aureus and Escherichia coli bacteria is the A. bilimbi L. plant. This research aims to examine the antibacterial effects of ethanol extract from A. bilimbi L.  leaves against S. aureus and E. Coli resistant antibiotic. The extraction process was conducted  using 70% ethanol through the maceration method. Subsequently, ethanol extract was prepared in a series of concentrations: 1.25%, 2.5%, 5%, and 10% w/v. The antibacterial activity test was performed using the agar diffusion method on Mueller Hinton Agar medium with test bacteria, E. coli, and S. aureus, which were already resistant to antibiotics. The research findings indicate that the ethanol extract from A. bilimbi L. leaves effectively inhibits the growth of test bacteria at a concentration of 1.25% (equivalent to 0.25 mg/paper disk). 
Analisis Zerumbone Dalam Zingiber zerumbet Dan Aktivitas Penghambatannya Terhadap Bakteri Mycobacterium tuberculosis: Analysis of zerumbone in Zingiber zerumbet and inhibitory activity against Mycobacterium tuberculosis Lallo, Subehan; Kasim, Syaharuddin; Tayeb, Rosany; Hasan, Asril Damiyanto; Sere, Hartina; Ismail, Ismail; Arifin, Tamsil
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 4 No. 2 (2018): (October 2018)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.802 KB) | DOI: 10.22487/j24428744.2018.v4.i2.11138

Abstract

Zerumbone has been reported for their several biological activities. In our interest to this compound, we have identified and analyzed its content in Zingiber zerumbet, a medicinal plant from Indonesian traditional medicine and investigated its inhibitory activity against Mycobacterium tuberculosis, a known infection bacteria of tuberculosis. Analysis of zerumbone was performed with densitometry to leave, rhizome, flower, and stem of Z. zerumbet which was extracted with various solvent system and extraction methods to determine the best method to isolate zerumbone from Z. zerumbet. Result showed that the highest zerumbone was in rhizome while was not observed in other part. Analysis with various solvent and extraction methods showed the highest yield of zerumbone can be extracted by n-hexane (maceration) and reflux extraction method (methanol). Furthermore, inhibitory activity of zerumbone against M. tuberculosis was tested using Lowenstein Jensen medium by counting the number of M. tuberculosis colony growth in medium. Resulted inhibitory activity of zerumbone at all test concentration (0.5, 0.1, 0.05, 0.01, 0.005%) with the growth of 10, 12, 14, 15, and 50 colonies of M. tuberculosis was observed, respectively. This is indicate that zerumbone can be used as an alternative choice for treatment tuberculosis in the future.