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All Journal FITOFARMAKA: Jurnal Ilmiah Farmasi Journal of Fundamental and Applied Pharmaceutical Science
Michael Raharja Gani
Fakultas Farmasi, Universitas Sanata Dharma
Chemistry Medicine & Pharmacology

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Stability Testing of Compounding Capsule Combination between Paracetamol and Tramadol in a Private Hospital Semarang Shirley Candra Kurniawan; Michael Raharja Gani; Sri Hartati Yuliani
Journal of Fundamental and Applied Pharmaceutical Science Vol 1, No 1 (2020): August
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jfaps.010103

Abstract

A combination of Paracetamol and Tramadol is used in mild to severe pain management. In a private Hospital Semarang, this combination is included in fast-moving drugs that it is frequently compounded and prepared in advance. The study aims to determine Beyond Use Date (BUD) in compounding capsules combination between Paracetamol and Tramadol samples. Beyond Use Date is a time limit indicating that a medicine beyond the expiration date must not be used and determined based on the results of stability testing. Samples were stored for 14 days in a tightly closed container far from direct sunlight in room temperature without AC (280C) and without silica gel, room temperature with AC (250C) without silica gel, and room temperature with AC (250C) with silica gel. Furthermore, samples underwent physical and chemical stability testing. Physical stability testing was conducted using organoleptic testing by observing direct changes from the powder color in capsules, capsules form, and scent on the samples. Meanwhile, chemical stability testing was conducted by determining the content of active ingredient from the samples using reversed-phase HPLC method and C18 as the stationary phase, methanol and aquabidest (40:60) as the mobile phase, wavelength 271 nm, flow rate 0.6 mL/min and injection volume 10 μL. The result shows that samples were physically stable for being able to retain the original physical properties showed by consistent powder color and capsules form, and there was no available scent. However, the chemical stability testing method was unable to separate and quantify the content of Paracetamol, Tramadol, and formed degradation products. It can be concluded that Beyond Use Date based on organoleptic and chemical stability testing for 14 days of compounding capsules combination between Paracetamol and Tramadol could not be determined. Nevertheless, this study showed favorable storage conditions in a tightly closed container far from direct sunlight in Room Temperature with AC (250C) with Silica Gel.
VALIDASI DAN PENETAPAN KADAR SENYAWA RUTIN PADA EKSTRAK ETANOL DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DENGAN METODE KCKT Theodorus Rexa Handoyo; Go Andrew Purnomo; Chelsia Devina Maryanto; Michael Raharja Gani
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 12, No 1 (2022): FITOFARMAKA: Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v12i1.3596

Abstract

Binahong (Anredera cordifolia (Ten.) Steenis) merupakan salah satu tanaman herbal yang sering digunakan untuk pengobatan. Penelitian ini bertujuan untuk menetapkan kadar rutin pada ekstrak etanol daun binahong menggunakan kromatografi cair kinerja tinggi sebagai proses standardisasi ekstrak bahan alam dengan senyawa rutin sebagai penanda. Pada penelitian ini rutin digunakan sebagai senyawa penanda. Simplisia daun binahong diekstrak menggunakan metode digesti dengan etanol 96% sebagai pelarut. Ekstraksi dilakukan pada suhu 50˚C dengan kecepatan pengadukan 200 rpm, dan ekstrak dipekatkan hingga tersisa 25% volume. Ekstrak kemudian diekstraksi cair cair menggunakan pelarut n-heksan dan diambil bagian etanolnya. Analisis dilakukan menggunakan Kromatografi Cair Kinerja Tinggi (KCKT). Optimasi dilakukan menggunakan fase gerak metanol, asetonitril, air serta beberapa variasi laju alir. Dilakukan validasi menggunakan parameter selektivitas, linearitas, Limit of Detection (LOD), Limit of Quantification (LOQ), akurasi dan presisi. Komposisi fase gerak terdiri dari metanol : asetonitril : akuabides pada perbandingan 30 : 10 : 60 dengan laju alir 0,7 mL/ menit. Deteksi dilakukan pada panjang gelombang 272 nm. Hasil penelitian memenuhi parameter validasi yaitu selektivitas dengan nilai resolusi 1,676, persamaan kurva baku y = 14672x - 2408 dengan nilai r sebesar 0,9975, nilai LOD dan LOQ adalah 0,104 dan 0,316 ppm. Nilai persen perolehan kembali pada akurasi didapatkan pada rentang 90,77 – 104,47% dan presisi kurang dari 6% untuk pengujian dalam 1 hari dan hari yang berbeda. Kadar rutin yang diperoleh sebesar 0,014 ± 0,000704 mg/g ekstrak dan nilai koefisien variasi sebesar 5,17%.
Co-Authors Chelsia Devina Maryanto Go Andrew Purnomo Shirley Candra Kurniawan Sri Hartati Yuliani Theodorus Rexa Handoyo