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All Journal Sains Natural: Journal of Biology and Chemistry Jurnal Kartika Kimia
Sari Purbaya
Jurusan Kimia, Fakultas Sains dan Informatika, Universitas Jenderal Achmad Yani
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education

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Antibacterial Activity of Ethyl Acetate Extract of Red Ginger (Zingiber officinale Roscoe var. Sunti) Against Bacteria of Staphylococcus aureus and Escherichia coli Sari Purbaya; Lilis Siti Aisyah; Jasmansyah Jasmansyah; Wenny Eliza Arianti
Jurnal Kartika Kimia Vol 1 No 1 (2018): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.477 KB) | DOI: 10.26874/jkk.v1i1.12

Abstract

Red ginger rhizome (Zingiber officinale Roscoe var. Sunti) is one of the herbs that is widely used as traditional medicine. The present study was to determine the secondary metabolites contained in red ginger rhizome plants and antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria. A total of 483.2 grams of red ginger powder was extracted by maceration method obtained from ethyl acetate extract 15.85 g. Antibacterial activity of red ginger extract against S. aureus and E. coli using microdilution method showed that ethyl acetate extract could inhibit S. aureus growth by MIC (Minimum Inhibitory Concentration) 6.3% and MBC (Minimum Bactericidal Concentration) 25% and extract Ethyl acetate red ginger rhizome can Minimum MIC (E. Inhibition Concentration) E. coli with a minimum inhibitory concentration of 25% and MBC (Minimum Bactericidal Concentration) 50%. The results showed that the red ginger rhizome ethyl acetate extract was the most influential and had more potential to inhibit the growth of Gram positive bacteria compared to inhibition of Gram negative.
Usnic Acid Isolated from Lichen Usnea sp. from Ciwidey, West Java Dewi Meliati Agustini; Riga Riga; Sari Purbaya; Eva Selviana; Muhammad Habibul Ikhsan
JURNAL SAINS NATURAL Vol. 13 No. 1 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i1.463

Abstract

Indonesia is a tropical country that has a variety of plants. Plants are one potential source of medicine. One of them is Lichen Usnea sp., which is used by the community to treat several diseases, so further research is needed to study the chemical composition contained and other potentials of this plant. Research on the chemical constituent of Lichen Usnea sp. consists of various stages: sample preparation, maceration using methanol, fractionation by Vacum Liquid Chromatography (VLC) and radial chromatography guided by Thin Layer Chromatography (TLC) eluting with n-hexane, chloroform and ethyl acetate obtaining an isolated compound. The isolated compound was analyzed by various spectroscopic data (UV-Vis, FT-IR, 1H-NMR and 13C-NMR). Based on the spectra, the isolated compound was usnic acid.Keywords:  Usnic acid;  Lichen; Usnea sp.ABSTRAKIsolat Asam Usnat dari Lichen Usnea sp. Asal Ciwidey, Jawa BaratIndonesia adalah negara tropis dengan banyaknya spesies tumbuhan. Tumbuhan adalah salah satu sumber bahan baku yang potensial untuk obat–obatan. Salah satunya Lichen Usnea sp. yang digunakan masyarakat dalam pengobatan beberapa penyakit, sehingga diperlukan penelitian lebih lanjut untuk mengetahui senyawa kimia yang terkandung dan potensi lainnya dari tanaman ini. Penelitian kandungan kimia dari Lichen Usnea sp. asal Ciwidey, Jawa Barat, terdiri dari berbagai tahapan, yaitu, persiapan sampel, maserasi dengan pelarut metanol, fraksinasi dan pemurnian dengan metode Kromatografi Cair Vakum (KCV) dan kromatografi radial yang dipandu dengan Kromatografi Lapis Tipis (KLT) dengan eluen n-heksana, kloroform dan etil asetat dan menghasilkan satu senyawa. Senyawa yang diperoleh tersebut dianalisis dengan berbagai data spektroskopi (UV, IR, 1H-NMR, dan 13C-NMR). Hasil karakterisasi menunjukkan bahwa senyawa tersebut adalah asam usnat.Kata kunci: Asam usnat; Lichen; Usnea sp.
Co-Authors Dewi Meliati Agustini Eva Selviana Jasmansyah Jasmansyah Lilis Siti Aisyah Muhammad Habibul Ikhsan Riga Riga Wenny Eliza Arianti