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All Journal Journal of Food and Pharmaceutical Science Indonesian Journal of Cancer Chemoprevention
Fortunella Tjondro
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia.
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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Leunca (Solanum nigrum L.) Herbs Ethanolic Extract Increase Cytotoxic Activity of Cisplatin on Hela Cervical Cancer Cells Istiaji, Raditya Prima; Fitria, Maya; Larasati, .; Tjondro, Fortunella; Maruti, Astrid Ayu; Setyowati, Erna Prawita; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Cervical cancer is one of leading causes of cancer death in women in the developing countries. The use of cisplatin as chemotherapy agent in cervical cancer is known to cause side effects and also resistance for long-term uses. One of the strategies to prevent cervical cancer based on combination agents is being developed. Leunca (Solanum nigrum L.) has been revealed to inhibit growth of human cancer cells. Therefore, it can be used in combination with cisplatin to reduce those side effects and prevent the occurrence of cell resistance. Ethanolic extract of Leunca Herb (ELH) and cisplatin were tested their cytotoxic effect on HeLa cervical cancer cell by using MTT assay to determine IC50 value. The combinationss of cisplatin-ELH were tested to determine the combination index (CI value).  The IC50 of ELH and cisplatin on HeLa cells were 227 µg/mL and 17 µM. rRespectively. Tthe study of combination resulted that almost all the index combinations were <0,9 showed  the effect of synergism combination. The Ooptimum concentration of combination was  1/8 IC50 cisplatin–1/8 IC50 ELH. The results indicated that ELH had a potency to be combination agent to enhance the activity of cisplatin on HeLa cervical cancer cells. Therefore, further study on its molecular mechanism needs to be explored.
Application of Primer LEP in Detecting Pork Adulteration in Meat Burger Using Hot-Start Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis Tjondro, Fortunella; ., Sismindari
Journal of Food and Pharmaceutical Sciences Vol 2, No 3 (2014): J. Food Pharm. Sci (Sept-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.125 KB) | DOI: 10.14499/jfps

Abstract

The development of pig species detection in food is increasing due to pork adulteration. Hitherto, the strongest method to detect and quantify pig presence in food is Real-Time Polymerase Chain Reaction. The object of this study is to know whether published primers that amplified leptin gene (LEP primer) could be used to detect and quantify pig’s presence in meat burger using Real-Time Polymerase Chain Reaction with dye intercalator- based detection. Genomic DNA isolation was done by proteinase K digestion.  Porcine DNA was amplified using LEP primer with Hot-Start Real-Time Polymerase Chain Reaction combined with Melting Curve Analysis. Condition of Real-Time PCR used in this experiment could amplify not only 152bp porcine leptin gene fragment with Tm value of 83.5oC but also 205bp cow’s leptin gene fragment with Tm 80oC. The result suggests that LEP primer is not a species-specific primer so it can’t be used to quantify pig’s presence in meat burger using Real-Time Polymerase Chain Reaction with dye intercalator-based detection.
Co-Authors . Larasati Astrid Ayu Maruti Edy Meiyanto Erna Prawita Setyowati Hargiani, Fransisca Xaveria Raditya Prima Istiaji Sismindari .