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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Kusbiyanto Kusbiyanto
Fakultas Biologi Unsoed
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Bioekologi Udang Macrobrachium spp. di Sungai Banjaran Kabupaten Banyumas Kusbiyanto Kusbiyanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 1 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.1.914

Abstract

Shrimp species diversity in River Banjaran is in a trend to reduce from time to time. In order to verify this trend, a research on bioecology of freshwater shrimp Macrobrachium spp. In River Banjaran was done using survey method. River’s body was divided into three different areas according to their environmental condition. On each area, six sampling stations were defined with the distance among stations were ± 0.5 km. The shrimps were collected using electroshocker, ancho, and waring. The samples were preserved in 70% ethanol and were identified. Only four species of shrimps were found during the survey, those are Macrobrachium cowlesi, M. idea, M. esculentum, and M. oenone.  Shrimp relative abundance among areas was similar with the highest relative abundance was found for M. cowlesi. Sex ratio analysis for all species showed that male individuals were more abundance than the female ones. This result provided a picture that species diversity of freshwater shrimps in River Banjaran is still in continuous decrease.  Therefore, it is necessary to make some efforts to conserve the rest of Machrobrachium species in Banjaran River.
Karakterisasi Molekuler Ikan Gurami Soang (Osphronemus gouramy Lac.) yang Mati pada Rentang Waktu Berbeda Menggunakan PCR-RFLP Gen Major Histocompatibility Complex Kelas II B Jaka Tri Spetiawan; Agus Nuryanto; Hendro Pramono; Kusbiyanto Kusbiyanto; Petrus H. Tjahja Soedibja
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.373

Abstract

Gurami (Osphronemus gouramy Lac.) is a popular fish species among Indonesian people. Several Gurami strains have been cultivated by fish farmer, one of which is Gurami Soang. This strain is belived to have a faster growth rate compared to other strains. However, like other strains, the fingerling of Soang strain have also a low survival and suceptible to disease, especially that caused by Aeromonas hydrophila infection. It has been proved that seeds from a single spawning event show varibale disease resistance. The difference in resistance among individuals is suggested related to the difference in their genetic component. One of the genes responsible for resistance is Major Histocompatibility Complex (MHC) class II B gene. Variability in resistance can be analyzed by using PCR - RFLP technique. PCR-RFLP is a technique that can produce a specific DNA fragments by PCR, followed by cutting the PCR product using restriction enzymes to describe the presence or absence of restriction sites in DNA fragments. This research aims to determine genetic marker to differiantiate between resitant and irresistant individual of Gurami Soang infected by A. hydrophila which die at a different time priod based on PCR-RFLP MHC class IIB gene. The study used survey method with purposive random sampling. The Data of PCR-RFLP band patterns were analyzed descriptively. The result indicated that cutting of the MHC class II B gene using HinfI produce two RFLP bands with 300 bp and 100 bp length in all samples. Meanwhile, the MHC IIB gene was not cuted by PstI, HindIII, BamHI and EcoRI enzymes forall samples. These mean that MHC II gene in all individuals were monomorphic. Therefore,it can be concluded that there is no specific genetic marker to differentiate gurami soang individulas which was dying in different time periods.
Co-Authors Agus Nuryanto Hendro Pramono Jaka Tri Spetiawan Petrus H. Tjahja Soedibja