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All Journal Scientific Contributions Oil and Gas
Zulkifliani Zulkifliani
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Chemical Engineering, Chemistry & Bioengineering Energy

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BIOREMEDIATION OF CRUDE OIL CONTAMINATED SEAWATER WITH THE APPLICATION OF BIOSURFACTANT AND BIOSTIMULATION Zulkifliani Zulkifliani; Atiyah F F Yumna; Subagiyo Subagiyo
Scientific Contributions Oil and Gas Vol 41 No 2 (2018)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.41.2.340

Abstract

Petroleum is one of the pollutant sources that can be found in the oceans and has a negative impact onthe marine ecosystem. Special conditions are needed to reduce the pollutant, one of the processes that occursis biodegradation, which is the process of degradation of petroleum hydrocarbon components by the activityof microorganisms so that the marine ecosystem returns to normal. Remediation of seawater contaminatedwith petroleum through the application of biosurfactants and biostimulation is one way to increase theeffectiveness of petroleum biodegradation. To examine these aspects laboratory-scale experiments werecarried out using basic seawater media which added oil pollutants. There are four treatments: P0 (basicmedia without the addition of biosurfactant and nutrients), P1 (addition of biosurfactant), P2 (additionof biosurfactant and nutrients), and P3 (addition of nutrients). The results of observations for 21 daysof incubation showed that the addition of biosurfactant treatment did not show a signifi cant increase110in biodegradation. Signifi cant increase in biodegradation occurs in the treatment with the addition ofbiostimulants and more degradation if biostimulation is combined with biosurfactant.
BIODEGRADATION ENHANCEMENT OF OILY WASTEWATER BY OZONATION METHOD Syafrizal Syafrizal; Zulkifliani Zulkifliani; Fami Adetyas Maulana Pratama; Setijo Bismo
Scientific Contributions Oil and Gas Vol 38 No 2 (2015)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.38.2.541

Abstract

This research is aimed at improving the ability of oily wastewater biodegradation through the ozonation method, where ozone will oxidize organic compounds in the oily wastewater that are diffi cult to be degraded by bacteria so that the structure becomes simpler. The ozonation process is done by using a corona-discharge ozonator and semi-batch reactor, and the process is varied by ozone dose and pH value. The ozonation process is then followed by a biodegradation process, where the bacteria that is used is Pseudomonas sp. This study also observed the effect of surfactants in supporting the biodegradation processes. An indicator of research success is the increase in the biodegradation percentage of the sample, followed by an increase in the value of the constant rate of bacterial growth. The research results shows that the ozonation method and the use of surfactant can support the oily wastewater biodegradation process. This is shown by the value of the biodegradation percentage and the constant rate of bacterial growth results, in which respective values are able to reach 86,787% and 0,339 at the optimal operating conditions (ozone dose of 0.53 g/h; pH 7; Bios-H surfactant concentration 500 ppm).
Reduction Of Bacteria Cells Viability In Injection Water By Using Ammonium Chloride Zulkifliani Zulkifliani; Usman Usman
Scientific Contributions Oil and Gas Vol 34 No 1 (2011)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.34.1.789

Abstract

Ammonium chloride is one of the biocides used to reduce viability of bacterial cells in  produced water from oil wells that will be applied as injection water. The advantage of this biocide is having high epication penetrate the cell wall, causing death of bacteria cells (bacteriocidal). Tests for antibacterial activity was done by using the API RP-38 method by adding ammonium chloride into the produced water to obtain the final concentrations of 4, 8, 16, 32, 64, and 128 mg/L. Laboratory test results showed that giving ammonium chloride at a concentration of 64 mg/L can significantly reduced the viability of bacterial cells, including groups of aerobic and anaerobic bacteria.
The Effect Of Biocides Addition Against Morphology And Size Distribution Of Bacteria Cells In Injection Water Zulkifliani Zulkifliani; Junita Trivianty Musu
Scientific Contributions Oil and Gas Vol 34 No 1 (2011)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.34.1.792

Abstract

Produced water is the water produced along with oil and gas. Produced water contains small to large quantities of suspended solids, such as corrosion material, scale, clay, wax, oil residues, and also bacteria and their metabolites. Produced water which is re-injected into the reservoir without having proper water treatment can lead to rock plugging and reservoir damage. One of the causes of reservoir damage is the occurrence of bacteria cells and their metabolites. Biocides are used to reduce the viability of bacteria cells in produced water to be applied as water injection. The aim of this study is to understand the effect of biocides on morphology and the size distribution of bacteria cells in injected water. Observations with scanning electron microscope (SEM) and laboratory tests showed that provision of biocides can cause damage of cytoplasmic membrane on bacteria cells or cells lyses and significantly reduced size distribution of bacteria cells.
Co-Authors Atiyah F F Yumna Fami Adetyas Maulana Pratama Junita Trivianty Musu Setijo Bismo Subagiyo Subagiyo Syafrizal Syafrizal Usman Usman