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Isolasi, Purifikasi dan Immunogenitas Protein Outer Membran Vibrio Alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Desrina Desrina; Arief Taslihan; Ambariyanto Ambariyanto; Ervia Yudiati; Yulius Docang Casessar; Raden Bagus Sugio Sumanta; Triyanto Triyanto; Hotnida Junita Situmeang; Langkah Sembiring
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.57

Abstract

The main objectives of this research were to isolate, purify and determine the immunogenicity of the outer membrane protein of V. alginolyticus. The outer membrane protein was isolated by sonication, sodium dodecyl sulphate-polyacrylamide agarose gel electrophoresis (SDS-PAGE) and purified by electroelusion method. Four outer membrane proteins were obtained: namely 32.0; 37.83; 64.13 and 73.43 kDa. Its immunogenicity in grouper Epinephelus fuscoguttatus was compared to that of bacterin of the same isolate. The immunogenicity test was conducted by intra peritoneal injection method. Each protein was dissolved in sterile Phospate Buffer Saline (PBS) and Freund’s Complete Adjuvant (FCA) (1:1) and injected 5 µg/ fish (fish weight 10-15 g). Bacterin (106, 107 and 108 cells/ml) was prepared in the same manner and injected at dosage of 0,2 ml/fish. Control fish were injected with 0.2 ml sterile PBS (pH 7.2). Booster was done a week later by injecting protein or bacterin with the Freund’s Incomplete Adjuvant (FIA). The agglutination test of antibody produced recognized cell surface protein of the whole cell of V. alginolyticus. Outer membrane protein 73.43 kDa was more immunogenic than the rest of proteins and bacterin, based on agglutinating antibodi titer.
PENGEMBANGAN METODE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION OF DNA DAN APLIKASINYA UNTUK DETEKSI KOI HERPES VIRUS PADA BEBERAPA JENIS IKAN Murwantoko Murwantoko; Triyanto Triyanto; Dimas A. Pamungkas
Jurnal Perikanan Universitas Gadjah Mada Vol 12, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.443 KB) | DOI: 10.22146/jfs.2948

Abstract

The purposes of this experiment were to establish loop-mediated isothermal amplification of DNA ( LAMP) method and its application to observe the presence and duration of koi herpes virus ( KHV) in freshwater fishes after infection. The infection was carried out using 4-6 cm length of java barb (Barbodes gonionotus), grass carp (Ctenoparingodon idella), gold fish (komet) (Carassius auratus), tambaqui (Colossoma macropomum) and koi (Cyprinus carpio koi) fishes. Fishes were infected intraperitoneally with KHV inoculums and 2 fishes were sampled everyday. DNA was extracted from gill and used for diagnosis with LAMP assays. The result of this experiment showed LAMP assay can be established and gave 100 times more sensitive than conventional PCR assay. Koi was confirmed as a host of KHV. Java barb, tambaqui, gold fish and grass carp can serve as vector for KHV and the presence of KHV was detected in java barb and tambaqui for 4 days, goldfish and grass carp for 5 days after infection.
PERBEDAAN DAYA TAHAN NON SPESIFIK LIMA SPESIES IKAN AIR TAWAR TERHADAP INFEKSI Aeromonashydrophila H. Syakuri; Triyanto Triyanto; K. H. Nitimulyo
Jurnal Perikanan Universitas Gadjah Mada Vol 5, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9031

Abstract

Fish naturally has a non-specific defense against several pathogens. The non-specific defense is anatomical and physiological function that varies according to genetical and environmental factor. The differences of non-specific defense against Aeromonas hydrophila infection were studied in blackfinpacu (Colossoma macropomum), gourami (Osphronemus goramy), common crap (Cyprinus carpio), African catfish (Clarias gariepinus), and red nile tilapia (Orochromis sp.). This study also examined the differences on several parameters of non-specific defense, including differentiation anc number of leucocytes, serum total, titer of antibody, and antibacterial activity of skin mucus.The fishes were intramuscularly infected at the media lethal doses for common carp (7,4 x 108cfu/fish). Pathological changes, survival rate, and mean time to death were observed every day. The observation of non-specific defense parameters were carried out prior and at seven day after infection.The results showed thahblackfinpacu was the most resistant species against A. hydrophila infection. African catfish and red nile tilapia were most resistant than gourami and common carp. The antibody titer and percentage of thrombocytes were the causative factor for the difference of the resistence against A. hydrophila. The five fish species also showed differences on leucocytes number, monocytes and eosinophiles percentage, and serum total, but they did not showed the differences of neutrophile percentage. The skin mucus of all fish species did not exhibited antibacterial activity against A. hydrophila. This study also found increase in antibody titer and leucocytes number after A. hydrophila infection.
MONITORING PARASIT PADA BUDIDAYA IKAN DAN UDANG DI DAERAH ISTIMEWA YOGYAKARTA Triyanto Triyanto; Alim Isnansetyo
Jurnal Perikanan Universitas Gadjah Mada Vol 6, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9041

Abstract

Parasites often cause the great loss in aquaculture either in fish or shrimp aquacultures. The loss will become more serious when fish or shrimp are secondary infected especially by bacteria. The objective of this study was to identify parasites in fish and shrimp culture inYogyakartaSpecialProvince (DIY). Samples of fish and shrimp were collected from several aquaculture centers in this region including several fish and shrimp hatcheries. Observation of parasites was conducted microscopically and macroscopically.Results showed that giant tiger prawn (Penaeus monodon) cultured in DIY was often infected by Zoothamnium sp. and Gregarines sp.. In addition, giant freshwater prawn (Machrobrachium rosenbergii) was often infected by Apiosoma sp., Dactylogyrus sp., Epystilis sp., Glossatella piscicola, Gregarines sp. and Zoothamnium sp.. Moreover Apiosoma sp.,  Dactylogyrus sp., Gyrodactylus sp., Ichthyopthirius sp., and Trichodina sp. were found in nile (Oreochromis niloticus) culture. Similar parasites such as Apiosoma sp., Dactylogyrus sp., Henneguya sp. Ichtyoptirius sp. and Trichodina sp..were found in Osphronemus gaurami. Ichtyoptirius sp. and Trichodina sp. also were found in walking catfish (Clarias gariephinus). 
ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO Kamiso Handoyo Nitimulyo; Alim Isnansetyo; Triyanto Triyanto; Indah Istiqomah; Muhammmad Murdjani
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9053

Abstract

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.
EFEKTIVITAS VAKSIN POLIVALEN UNTUK PENGENDALIAN VIBRIOSIS PADA KERAPU TIKUS (Cromileptes altivelis) Kamiso Handoyo Nitimulyo; Alim Isnansetyo; Triyanto Triyanto; Muhammad Murdjani; Lili Sholichah
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9056

Abstract

The objective of this research was to know the effectiveness of polyvalen  Vibrio vaccine to control vibriosis in humpback grouper (Cromileptes altivelis). The effectiveness of vaccine was evaluated by the survival rate (SR), relative percent survival (RPS), mean time to death (MTD) as well as growth rate of vaccinated fish. This research consisted of 4 treatments (control, injection, immersion, and oral vaccinations) in quadruplicates. Injection  vaccination was conducted by intraperitoneal injection of polyvalen vaccine at 107 cells/fish. Immersion vaccination was done by immersing the fishes at 107 cells/ml for 30 minutes. Oral administration of vaccine was also carried out  at 107 cells/fish. One week after the first vaccination, second vaccination (booster) was carried out at the same dosage and by the same administration. One week after the second vaccination, fishes were challenged with 3.16x104 cells/fish of Vibrio ordalii 3J by intraperitoneal injection, and reared for 20 days post infection. Results indicated that polyvalen Vibrio vaccine increased SR (P<0.01) up to 100%. Vaccination was also able to delay MTD of fishes. However, the vaccination was not influence the growth rate of fish.
IDENTIFIKASI BAKTERI PATOGEN PADA KUDA LAUT (Hippocampus kuda) DI BALAI BUDIDAYA LAUT, LAMPUNG Yudha Trinoegraha Adiputra; Triyanto Triyanto; Namastra Probosunu
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9064

Abstract

Sea horse (Hippocampus kuda) is one of the ornamental marine organisms and raw material of traditional medicine. Since 1993, Seafarming Development Centre, Lampung has pioneered a research and  culture  of sea horse in Indonesia. The serious problem in the culture of sea horse is pathogenic bacteria caused death of juveniles and broodstocks. The objective of this study was to identify pathogenic bacteria isolated from  sea horse in Seafarming Development Centre. Koch Postulate test was carried out, and then the pathogenic bacteria were identifed by morphological and biochemical tests. Results showed that from a total of 6 bacterial strains isolated from diseased sea horse, 3 strains were pathogenic bacteria to sea horse. These 3 pathogenic bacteria caused identical disease signs with the initial disease signs when the bacteria were isolated.  Morphological and biochemical tests suggested that  the pathogenic bacteria could be identified to be Vibrio fluvialis, V. alginolyticus and V. hollisae. 
Co-Authors Alim Isnansetyo Ambariyanto Ambariyanto Arief Taslihan Desrina Desrina Dimas A. Pamungkas Ervia Yudiati Hotnida Junita Situmeang INDAH ISTIQOMAH K. H. Nitimulyo Kamiso Handoyo Nitimulyo Langkah Sembiring Lili Sholichah Muhammad Murdjani Muhammmad Murdjani Murwantoko . Namastra Probosunu Raden Bagus Sugio Sumanta Syakuri, Hamdan Yudha Trinoegraha Adiputra Yulius Docang Casessar