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Kemangkusan Nematoda Entomopatogen Steinernema carpocapsae terhadap Hama Penggerek Umbi/Daun (Phthorimaea operculella Zell.) Kentang Uhan, T S
Jurnal Hortikultura Vol 18, No 1 (2008): Maret 2008
Publisher : Indonesian Center for Horticultural Research and Development

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Abstract

ABSTRAK. Penelitian ini bertujuan untuk mempelajari patogenisitas nematoda Steinernema carpocapsae terhadap larva Phthorimaea operculella di rumah kaca. Penelitian dilaksanakan di Rumah Kaca Balai Penelitian Tanaman Sayuran Lembang, Kabupaten Bandung mulai Agustus 2003-Agustus 2004. Penelitian menggunakan rancangan acak kelompok dengan 6 perlakuan dan 4 ulangan. Pengujian dilakukan menggunakan metode kertas saring dan penyemprotan. Perlakuan yang diuji yaitu 4 macam tingkat kepadatan populasi nematoda S. carpocapsae (200, 400, 800, dan 1.600 JI/ml), insektisida pembanding, dan kontrol. Hasil penelitian menunjukkan bahwa metode kertas saring dengan kepadatan S. carpocapsae 800 JI/ml pada 144 jam setelah aplikasi, efektif dalam mengendalikan larva P. operculella pada tanaman kentang di rumah kaca dengan mortalitas 100%, sedangkan pada metode penyemprotan dengan kepadatan S. carpocapsae 800 dan 1.600 JI/ml pada 144 jam setelah aplikasi dengan mortalitas 97,5 dan 100% berturut-turut.ABSTRACT. 2008 Effctivit Uhan, T.S. 2008. The Effectivity of Steinernema carpocapsae Against Pototatoto Tuber Mototh (Phthorimaea operculella Zell.).The objective of this research was to find out the pathogenicity of Steinernema carpocapsae nematodes Lembang strains on mortality of Phthorimaea operculella on potato plant in the greenhouse. The experiment was carried out in the Laboratory of Pest and in the Greenhouse of Indonesian Vegetable Research Institute, Lembang, Bandung, at 1,250 m asl, from August 2003 to August 2004. The experiment was arranged in a randomized block design with 6 treatments and 4 replications. Testing was done by using filter paper method and spraying method. The treatments were 4 level density of S. carpocapsae nematodes (200, 400, 800, and 1,600 JI/ml), one insecticide as comparison, and control. The results showed that filter paper method with density of S. carpocapsae 800 JI/ml at 144 hours after application was effective to control larvae of P. operculella on potato plant in a greenhouse with mortality of 100%, while spraying method with density of S. carpocapsae 800 and 1,600 JI/ml at 144 hours after application caused mortality 97.5% and 100% of P. operculella, respectively
Efektivitas Aplikasi Kombinasi Steinernema carpocapsae dan Biopestisida Bacillus thuringiensis terhadap Mortalitas Crocidolomia pavonana F. pada Tanaman Kubis di Rumah Kaca Uhan, T S; Sulastrini, I
Jurnal Hortikultura Vol 18, No 1 (2008): Maret 2008
Publisher : Indonesian Center for Horticultural Research and Development

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Abstract

ABSTRAK. Percobaan dilaksanakan di Rumah Kaca Balai Penelitian Tanaman Sayuran Lembang dari bulan Oktober sampai Desember 2006. Percobaan ini bertujuan untuk mengetahui pengaruh penggunaan kombinasi Steinernema carpocapsae dan Bacillus thuringiensis terhadap mortalitas larva Crocidolomia pavonana pada tanaman kubis di rumah kaca. Percobaan dilakukan menggunakan rancangan acak kelompok pola faktorial dengan 2 faktor dan 3 ulangan. Faktor pertama yaitu S. carpocapsae dengan 4 taraf yaitu 0, 400, 800, dan 1.600 JI/tanaman sedangkan faktor kedua yaitu konsentrasi B. thuringiensis dengan 3 taraf yaitu 0, 0,1, dan 0,2 g/100 ml. Hasil percobaan menunjukkan bahwa S. carpocapsae dan B. thuringiensis lebih baik apabila digunakan dengan cara dicampurkan daripada digunakan secara tunggal dalam mengendalikan larva C. pavonana, karena dapat menyebabkan mortalitas lebih tinggi daripada secara tunggal. Kombinasi 400 JI/tanaman S. carpocapsae dengan 0,1 g/100 ml B.thuringiensis menyebabkan mortalitas larva 53,33%, kombinasi 800 JI/tanaman S. carpocapsae dengan 0,1 g/100 ml B. thuringiensis menyebabkan mortalitas 66,67%, dan kombinasi 1.600 JI/tanaman dengan 0,1 g/100 ml B. thuringiensis menyebabkan mortalitas 90%. Sedangkan mortalitas tertinggi adalah pada perlakuan kombinasi 1.600 JI/tanaman S. carpocapsae dengan 0,2 g/100 ml B. thuringiensis menyebabkan mortalitas 100% pada pengamatan 96 jam setelah aplikasi.ABSTRACT. Uhan, T.S. and I. Sulastrini. 2008. Effectivity of Mixture of Steinernema carpocapsae and Bacillus thuringiensis Application on the Mortality of Crocidolomia pavonana F. on Cabbage in the Greenhouse. A greenhouse experiment was conducted at the Indonesian Vegetable Research Institute at Lembang from October to December 2006. The aim of this study was to observe the effffect of difffferent concentrations of S. carpocapsae and B. thuringiensis mixture which is effffective against C. pavonana larvae. The experiment was arranged in a factorial randomized block design with 2 factors and 3 replications. The first factor was the population density of S. carpocapsae, with 4 levels: 0, 400, 800, and 1,600 JI/plant, while the second factor was the concentration of B. thuringiensis, with 3 levels: 0, 0.1, and 0.2 g/100 ml. The results showed that S. carpocapsae and B. thuringiensis were much better than that of control C. pavonana if they were applied in mixture rather than applied in single treatment. Combination of 400 JI/plant S. carpocapsae with 0.1 g/100ml B. thuringiensis caused 53.33% mortality of C. pavonana larvae; 800 JI/plant S. carpocapsae with 0.1 g/100 ml caused 66.67% mortality of C. pavonana; and 1,600 JI/plant S. carpocapsae with 0.1 g/100 ml caused 90% mortality of C. pavonana. The highest mortalitity (100%) was showen by the treatment of 1.600 JI/plant of S. carpocapsae mixed with 0.2 g/100 ml B. thuringiensis at 96 hours after application.
Keefektifan Nematoda Entomopatogen Steinernema carpocapsae (Rhabditida:Steinernematidae) Isolat Lembang terhadap Mortalitas Larva Agrotis ipsilon Hufn. (Lepidoptera:Noctuidae) pada Tanaman Kubis di Rumah Kaca Uhan, T S
Jurnal Hortikultura Vol 18, No 2 (2008): Juni 2008
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Penelitian bertujuan untuk mengetahui keefektifan nematoda entomopatogen Steinernema carpocapsae isolat Lembang pada mortalitas larva Agrotis ipsilon pada tanaman kubis di rumah kaca. Percobaan dilaksanakan di Laboratorium Hama dan Rumah Kaca Balai Penelitian Tanaman Sayuran, Lembang, Kabupaten Bandung. Rancangan yang digunakan adalah acak kelompok dengan 7 perlakuan dan 4 ulangan. Perlakuan yang diuji adalah 5 macam perlakuan tingkat kepadatan populasi nematoda S. carpocapsae (325; 650; 1.300; 2.600; dan 5.200 JI/ml), pestisida sipermetrin 0,5 ml/l, dan kontrol. Hasil penelitian menunjukkan bahwa nematoda entomopatogen S. carpocapsae isolat Lembang mulai tingkat kepadatan 1.300 JI/ml efektif dalam mengendalikan larva A. ipsilon mengakibatlan mortalitas sebesar 56,11% dan mengurangi tingkat kerusakan tanaman kubis sebesar 47,50% pada 96 jam setelah aplikasi.ABSTRACT. Uhan, T.S. 2008. Effectiveness of Entomopathogenic Nematodes Steinernema carpocapsae (Rhabditida: Steinernematidae) Lembang Strain Against the Mortality of Agrotis ipsilon Hufn. (Lepidoptera: Noctuidae) on Cabbage in the Greenhouse. The purpose of this experiment was to study the effectiveness of entomopathogenic nematodes S. carpocapsae strain Lembang on the mortality of Agrotis ipsilon Hufn. larvae on cabbage in the greenhouse. The experiment was carried out in the Laboratory of Entomology and the Greenhouse of Indonesian Vegetable Research Institute, in Lembang, District of Bandung. The experiment was arranged in a randomized block design with 7 treatments and 4 replications. The treatments were 5 level of entomopathogenic nematodes population density, i.e. 325, 650, 1,300, 2,600, and 5,200 JI/ml, pesticide sipermethrine 0.5 ml/l, and control. The results of this research showed that entomopathogenic nematodes with population density of 1,300 JI/ml was effective to control A. ipsilon larvae, caused 56.11 % mortality and reduced damage up to 47.50% at 96 hours after treatment.
Bioefikasi Beberapa Isolat Nematoda Entomopatogenik Steinernema spp. terhadap Spodoptera litura Fabricius pada Tanaman Cabai di Rumah Kaca Uhan, T S
Jurnal Hortikultura Vol 18, No 2 (2008): Juni 2008
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Percobaan dilakukan di Rumah Kaca Balai Penelitian Tanaman Sayuran dari bulan Agustus sampai Oktober 2003. Percobaan ini bertujuan mengetahui isolat dan tingkat kepadatan populasi entomopatogen yang efektif terhadap larva Spodoptera litura pada tanaman cabai. Percobaan menggunakan rancangan acak kelompok dengan 21 perlakuan dan 4 ulangan. Perlakuan yang diuji yaitu 5 macam isolat Steinernema spp. dari Jombang, Lembang, Medan, Solo, dan Yogyakarta. Masing-masing isolat dicoba dengan 4 tingkat kepadatan populasi nematoda, yaitu (200, 400, 800, dan 1.600 JI/ml serta kontrol. Hasil percobaan menunjukkan bahwa terdapat perbedaan infektivitas antara Steinernema spp. isolat Jombang, Lembang, Medan, Solo, dan Yogyakarta. Isolat Lembang merupakan isolat yang paling infektif yang menyebabkan mortalitas tertinggi terhadap S. litura pada kepadatan 200, 400, dan 800 JI/ml, masing-masing sebesar 23,9, 51,1, dan 78,3% pada 120 jam setelah aplikasi dan LT50 yang terpendek yaitu 33,7565 jam setelah aplikasi.ABSTRACT. Uhan, T.S. 2008. Bioefficacy of Some Strains of Entomopathogenic Nematode Steinernema spp. Against Spodoptera litura Fabricius on Red Chili in the Greenhouse. The objective of this research was to study the infection capacity of entomopathogenic nematodes Steinernema spp. strains Jombang, Lembang, Medan, Solo, and Yogyakarta on Spodoptera litura on red chili in the greenhouse. The experiment was carried out in the Laboratory of Pest and in the Greenhouse of Indonesian Vegetable Research Institute at Lembang, Bandung District, from August-October 2003. The experiment was arranged in a randomized block design with 21 treatments and 4 replications. The treatments were 5 strains of Steinernema spp. (strain Jombang, Lembang, Medan, Solo, and Yogyakarta) with 4 level of nematode population (200, 400, 800, 1.600 JI/ml), and control. The results showed that there were differences in the capacity of infection among the strain of Steinernema spp. tested. Strain Lembang was the most infectious, which caused the highest mortality on S. litura. Population of Steinernema spp. at 200, 400, and 800 JI/ml caused mortality of S. litura up to 23.9; 51.1; and 78.3% respectively, at 120 hours after application. The LT50 of strain Lembang was also the shortest (33.7565 hours after application).
Efikasi Ekstrak Kasar Baculovirus Crocidolomia pavonana terhadap Ulat Krop Kubis di Rumah Kaca Uhan, T S
Jurnal Hortikultura Vol 17, No 3 (2007): September 2007
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Penelitian bertujuan menentukan konsentrasi Baculovirus Crocidolomia pavonana (BVCp) yang paling baik dalam menekan perkembangan Crocidolomia pavonana. Penelitian dilaksanakan di Laboratorium Hama Penyakit dan Rumah Kaca Balai Penelitian Tanaman Sayuran, Lembang, Bandung, dari bulan November 2001 sampai Februari 2002. Rancangan yang digunakan adalah acak kelompok dengan 6 perlakuan dan 4 ulangan. Perlakuan yang diuji 4 macam konsentrasi ekstrak larva C. pavonana yang terinfeksi oleh BVCp (5, 10, 20, dan 40 ekor per l air), formulasi Bacillus thuringienis (Dipel WP® 2,00 g/l), dan kontrol. Hasil penelitian menunjukkan bahwa setelah 6 hari (144 jam) aplikasi ekstrak 40 larva C. pavonana yang terinfeksi oleh BVCp per l air (116,64 x 1010 PIBs/ml) menyebabkan mortalitas larva tertinggi, yaitu 94,45%, dengan LT50 yang tercepat yaitu 64,81 jam setelah aplikasi.ABSTRACT. Uhan, T.S. 2007. The Effect of Baculovirus Crocidolomia pavonana (BVCp) Concentration Againts Crocidolomia pavonana Zell. in a Greenhouse. The purpose of this experiment was to determine the best concentration of BVCp in suppressing C. pavonana growth. This experiment was carried out at Laboratory of Pest and Diseases and in the Greenhouse of Indonesian Vegetable Research Institute, Lembang District, Bandung , from November 2001 to February 2002. The experiment was arranged in a randomized block design with 6 treatments and 4 replications. The treatments were 4 extract concentrations of C. pavonana larvae which were infected by BVCp (5, 10, 20, and 40 larvae per l of water), formulation of Bacillus thuringiensis (Dipel WP® 2.00 g/l), and control. The results showed that after 6 days (144 hours) of application the extract of 40 C. pavonana larvae infected by BVCp per l of water (116.64 x 1010 PIBs/ml) caused the highest larvae mortality (94.45%). The fastest LT50 was 64.81 hours after application.
Kemangkusan Nematoda Entomopatogen Steinernema carpocapsae terhadap Hama Penggerek Umbi/Daun (Phthorimaea operculella Zell.) Kentang Uhan, T S
Jurnal Hortikultura Vol 18, No 1 (2008): Maret 2008
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v18n1.2008.p%p

Abstract

ABSTRAK. Penelitian ini bertujuan untuk mempelajari patogenisitas nematoda Steinernema carpocapsae terhadap larva Phthorimaea operculella di rumah kaca. Penelitian dilaksanakan di Rumah Kaca Balai Penelitian Tanaman Sayuran Lembang, Kabupaten Bandung mulai Agustus 2003-Agustus 2004. Penelitian menggunakan rancangan acak kelompok dengan 6 perlakuan dan 4 ulangan. Pengujian dilakukan menggunakan metode kertas saring dan penyemprotan. Perlakuan yang diuji yaitu 4 macam tingkat kepadatan populasi nematoda S. carpocapsae (200, 400, 800, dan 1.600 JI/ml), insektisida pembanding, dan kontrol. Hasil penelitian menunjukkan bahwa metode kertas saring dengan kepadatan S. carpocapsae 800 JI/ml pada 144 jam setelah aplikasi, efektif dalam mengendalikan larva P. operculella pada tanaman kentang di rumah kaca dengan mortalitas 100%, sedangkan pada metode penyemprotan dengan kepadatan S. carpocapsae 800 dan 1.600 JI/ml pada 144 jam setelah aplikasi dengan mortalitas 97,5 dan 100% berturut-turut.ABSTRACT. 2008 Effctivit Uhan, T.S. 2008. The Effectivity of Steinernema carpocapsae Against Pototatoto Tuber Mototh (Phthorimaea operculella Zell.).The objective of this research was to find out the pathogenicity of Steinernema carpocapsae nematodes Lembang strains on mortality of Phthorimaea operculella on potato plant in the greenhouse. The experiment was carried out in the Laboratory of Pest and in the Greenhouse of Indonesian Vegetable Research Institute, Lembang, Bandung, at 1,250 m asl, from August 2003 to August 2004. The experiment was arranged in a randomized block design with 6 treatments and 4 replications. Testing was done by using filter paper method and spraying method. The treatments were 4 level density of S. carpocapsae nematodes (200, 400, 800, and 1,600 JI/ml), one insecticide as comparison, and control. The results showed that filter paper method with density of S. carpocapsae 800 JI/ml at 144 hours after application was effective to control larvae of P. operculella on potato plant in a greenhouse with mortality of 100%, while spraying method with density of S. carpocapsae 800 and 1,600 JI/ml at 144 hours after application caused mortality 97.5% and 100% of P. operculella, respectively
Efektivitas Aplikasi Kombinasi Steinernema carpocapsae dan Biopestisida Bacillus thuringiensis terhadap Mortalitas Crocidolomia pavonana F. pada Tanaman Kubis di Rumah Kaca T S Uhan; I Sulastrini
Jurnal Hortikultura Vol 18, No 1 (2008): Maret 2008
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v18n1.2008.p%p

Abstract

ABSTRAK. Percobaan dilaksanakan di Rumah Kaca Balai Penelitian Tanaman Sayuran Lembang dari bulan Oktober sampai Desember 2006. Percobaan ini bertujuan untuk mengetahui pengaruh penggunaan kombinasi Steinernema carpocapsae dan Bacillus thuringiensis terhadap mortalitas larva Crocidolomia pavonana pada tanaman kubis di rumah kaca. Percobaan dilakukan menggunakan rancangan acak kelompok pola faktorial dengan 2 faktor dan 3 ulangan. Faktor pertama yaitu S. carpocapsae dengan 4 taraf yaitu 0, 400, 800, dan 1.600 JI/tanaman sedangkan faktor kedua yaitu konsentrasi B. thuringiensis dengan 3 taraf yaitu 0, 0,1, dan 0,2 g/100 ml. Hasil percobaan menunjukkan bahwa S. carpocapsae dan B. thuringiensis lebih baik apabila digunakan dengan cara dicampurkan daripada digunakan secara tunggal dalam mengendalikan larva C. pavonana, karena dapat menyebabkan mortalitas lebih tinggi daripada secara tunggal. Kombinasi 400 JI/tanaman S. carpocapsae dengan 0,1 g/100 ml B.thuringiensis menyebabkan mortalitas larva 53,33%, kombinasi 800 JI/tanaman S. carpocapsae dengan 0,1 g/100 ml B. thuringiensis menyebabkan mortalitas 66,67%, dan kombinasi 1.600 JI/tanaman dengan 0,1 g/100 ml B. thuringiensis menyebabkan mortalitas 90%. Sedangkan mortalitas tertinggi adalah pada perlakuan kombinasi 1.600 JI/tanaman S. carpocapsae dengan 0,2 g/100 ml B. thuringiensis menyebabkan mortalitas 100% pada pengamatan 96 jam setelah aplikasi.ABSTRACT. Uhan, T.S. and I. Sulastrini. 2008. Effectivity of Mixture of Steinernema carpocapsae and Bacillus thuringiensis Application on the Mortality of Crocidolomia pavonana F. on Cabbage in the Greenhouse. A greenhouse experiment was conducted at the Indonesian Vegetable Research Institute at Lembang from October to December 2006. The aim of this study was to observe the effffect of difffferent concentrations of S. carpocapsae and B. thuringiensis mixture which is effffective against C. pavonana larvae. The experiment was arranged in a factorial randomized block design with 2 factors and 3 replications. The first factor was the population density of S. carpocapsae, with 4 levels: 0, 400, 800, and 1,600 JI/plant, while the second factor was the concentration of B. thuringiensis, with 3 levels: 0, 0.1, and 0.2 g/100 ml. The results showed that S. carpocapsae and B. thuringiensis were much better than that of control C. pavonana if they were applied in mixture rather than applied in single treatment. Combination of 400 JI/plant S. carpocapsae with 0.1 g/100ml B. thuringiensis caused 53.33% mortality of C. pavonana larvae; 800 JI/plant S. carpocapsae with 0.1 g/100 ml caused 66.67% mortality of C. pavonana; and 1,600 JI/plant S. carpocapsae with 0.1 g/100 ml caused 90% mortality of C. pavonana. The highest mortalitity (100%) was showen by the treatment of 1.600 JI/plant of S. carpocapsae mixed with 0.2 g/100 ml B. thuringiensis at 96 hours after application.
Keefektifan Nematoda Entomopatogen Steinernema carpocapsae (Rhabditida:Steinernematidae) Isolat Lembang terhadap Mortalitas Larva Agrotis ipsilon Hufn. (Lepidoptera:Noctuidae) pada Tanaman Kubis di Rumah Kaca T S Uhan
Jurnal Hortikultura Vol 18, No 2 (2008): Juni 2008
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v18n2.2008.p%p

Abstract

ABSTRAK. Penelitian bertujuan untuk mengetahui keefektifan nematoda entomopatogen Steinernema carpocapsae isolat Lembang pada mortalitas larva Agrotis ipsilon pada tanaman kubis di rumah kaca. Percobaan dilaksanakan di Laboratorium Hama dan Rumah Kaca Balai Penelitian Tanaman Sayuran, Lembang, Kabupaten Bandung. Rancangan yang digunakan adalah acak kelompok dengan 7 perlakuan dan 4 ulangan. Perlakuan yang diuji adalah 5 macam perlakuan tingkat kepadatan populasi nematoda S. carpocapsae (325; 650; 1.300; 2.600; dan 5.200 JI/ml), pestisida sipermetrin 0,5 ml/l, dan kontrol. Hasil penelitian menunjukkan bahwa nematoda entomopatogen S. carpocapsae isolat Lembang mulai tingkat kepadatan 1.300 JI/ml efektif dalam mengendalikan larva A. ipsilon mengakibatlan mortalitas sebesar 56,11% dan mengurangi tingkat kerusakan tanaman kubis sebesar 47,50% pada 96 jam setelah aplikasi.ABSTRACT. Uhan, T.S. 2008. Effectiveness of Entomopathogenic Nematodes Steinernema carpocapsae (Rhabditida: Steinernematidae) Lembang Strain Against the Mortality of Agrotis ipsilon Hufn. (Lepidoptera: Noctuidae) on Cabbage in the Greenhouse. The purpose of this experiment was to study the effectiveness of entomopathogenic nematodes S. carpocapsae strain Lembang on the mortality of Agrotis ipsilon Hufn. larvae on cabbage in the greenhouse. The experiment was carried out in the Laboratory of Entomology and the Greenhouse of Indonesian Vegetable Research Institute, in Lembang, District of Bandung. The experiment was arranged in a randomized block design with 7 treatments and 4 replications. The treatments were 5 level of entomopathogenic nematodes population density, i.e. 325, 650, 1,300, 2,600, and 5,200 JI/ml, pesticide sipermethrine 0.5 ml/l, and control. The results of this research showed that entomopathogenic nematodes with population density of 1,300 JI/ml was effective to control A. ipsilon larvae, caused 56.11 % mortality and reduced damage up to 47.50% at 96 hours after treatment.
Bioefikasi Beberapa Isolat Nematoda Entomopatogenik Steinernema spp. terhadap Spodoptera litura Fabricius pada Tanaman Cabai di Rumah Kaca T S Uhan
Jurnal Hortikultura Vol 18, No 2 (2008): Juni 2008
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v18n2.2008.p%p

Abstract

ABSTRAK. Percobaan dilakukan di Rumah Kaca Balai Penelitian Tanaman Sayuran dari bulan Agustus sampai Oktober 2003. Percobaan ini bertujuan mengetahui isolat dan tingkat kepadatan populasi entomopatogen yang efektif terhadap larva Spodoptera litura pada tanaman cabai. Percobaan menggunakan rancangan acak kelompok dengan 21 perlakuan dan 4 ulangan. Perlakuan yang diuji yaitu 5 macam isolat Steinernema spp. dari Jombang, Lembang, Medan, Solo, dan Yogyakarta. Masing-masing isolat dicoba dengan 4 tingkat kepadatan populasi nematoda, yaitu (200, 400, 800, dan 1.600 JI/ml serta kontrol. Hasil percobaan menunjukkan bahwa terdapat perbedaan infektivitas antara Steinernema spp. isolat Jombang, Lembang, Medan, Solo, dan Yogyakarta. Isolat Lembang merupakan isolat yang paling infektif yang menyebabkan mortalitas tertinggi terhadap S. litura pada kepadatan 200, 400, dan 800 JI/ml, masing-masing sebesar 23,9, 51,1, dan 78,3% pada 120 jam setelah aplikasi dan LT50 yang terpendek yaitu 33,7565 jam setelah aplikasi.ABSTRACT. Uhan, T.S. 2008. Bioefficacy of Some Strains of Entomopathogenic Nematode Steinernema spp. Against Spodoptera litura Fabricius on Red Chili in the Greenhouse. The objective of this research was to study the infection capacity of entomopathogenic nematodes Steinernema spp. strains Jombang, Lembang, Medan, Solo, and Yogyakarta on Spodoptera litura on red chili in the greenhouse. The experiment was carried out in the Laboratory of Pest and in the Greenhouse of Indonesian Vegetable Research Institute at Lembang, Bandung District, from August-October 2003. The experiment was arranged in a randomized block design with 21 treatments and 4 replications. The treatments were 5 strains of Steinernema spp. (strain Jombang, Lembang, Medan, Solo, and Yogyakarta) with 4 level of nematode population (200, 400, 800, 1.600 JI/ml), and control. The results showed that there were differences in the capacity of infection among the strain of Steinernema spp. tested. Strain Lembang was the most infectious, which caused the highest mortality on S. litura. Population of Steinernema spp. at 200, 400, and 800 JI/ml caused mortality of S. litura up to 23.9; 51.1; and 78.3% respectively, at 120 hours after application. The LT50 of strain Lembang was also the shortest (33.7565 hours after application).
Efikasi Ekstrak Kasar Baculovirus Crocidolomia pavonana terhadap Ulat Krop Kubis di Rumah Kaca T S Uhan
Jurnal Hortikultura Vol 17, No 3 (2007): September 2007
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v17n3.2007.p%p

Abstract

ABSTRAK. Penelitian bertujuan menentukan konsentrasi Baculovirus Crocidolomia pavonana (BVCp) yang paling baik dalam menekan perkembangan Crocidolomia pavonana. Penelitian dilaksanakan di Laboratorium Hama Penyakit dan Rumah Kaca Balai Penelitian Tanaman Sayuran, Lembang, Bandung, dari bulan November 2001 sampai Februari 2002. Rancangan yang digunakan adalah acak kelompok dengan 6 perlakuan dan 4 ulangan. Perlakuan yang diuji 4 macam konsentrasi ekstrak larva C. pavonana yang terinfeksi oleh BVCp (5, 10, 20, dan 40 ekor per l air), formulasi Bacillus thuringienis (Dipel WP® 2,00 g/l), dan kontrol. Hasil penelitian menunjukkan bahwa setelah 6 hari (144 jam) aplikasi ekstrak 40 larva C. pavonana yang terinfeksi oleh BVCp per l air (116,64 x 1010 PIBs/ml) menyebabkan mortalitas larva tertinggi, yaitu 94,45%, dengan LT50 yang tercepat yaitu 64,81 jam setelah aplikasi.ABSTRACT. Uhan, T.S. 2007. The Effect of Baculovirus Crocidolomia pavonana (BVCp) Concentration Againts Crocidolomia pavonana Zell. in a Greenhouse. The purpose of this experiment was to determine the best concentration of BVCp in suppressing C. pavonana growth. This experiment was carried out at Laboratory of Pest and Diseases and in the Greenhouse of Indonesian Vegetable Research Institute, Lembang District, Bandung , from November 2001 to February 2002. The experiment was arranged in a randomized block design with 6 treatments and 4 replications. The treatments were 4 extract concentrations of C. pavonana larvae which were infected by BVCp (5, 10, 20, and 40 larvae per l of water), formulation of Bacillus thuringiensis (Dipel WP® 2.00 g/l), and control. The results showed that after 6 days (144 hours) of application the extract of 40 C. pavonana larvae infected by BVCp per l of water (116.64 x 1010 PIBs/ml) caused the highest larvae mortality (94.45%). The fastest LT50 was 64.81 hours after application.