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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Journal of Applied Food Technology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Aquacultura Indonesiana Chalim Journal of Teaching and Learning Jurnal Pertanian Kepulauan
Ustadi Ustadi
Jurusan Perikanan UGM
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Languange, Linguistic, Communication & Media Other

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Aplikasi Campuran Alginat Dari Sargassum Crassifolium Dan Gum Sebagai Pengental Textile Printing Subaryono, Subaryono; Tazwir, Tazwir; Husni, Amir; Ustadi, Ustadi; Pranoto, Yudi
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): Desember 2015
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v10i2.225

Abstract

Penelitian aplikasi campuran alginat dari Sargassum crassifolium dan gum untuk meningkatkan viskositas alginat sebagai pengental pada textile printing telah dilakukan. Viskositas campuran alginat dengan guar gum, gum arab, dan locust bean gum diamati pada penyimpanan selama 8 jam. Produk terbaik diujikan sebagai pengental pada textile printing. Campuran alginat dengan guar gum pada perbandingan 90:10 dan 80:20 meningkatkan viskositas dan stabilitas alginat selama penyimpanan. Campuran alginat dengan gum arab dan locust bean gum akan menurunkan viskositas alginat sehingga tidak sesuai untuk aplikasi textile printing. Aplikasi campuran alginat dengan guar gum 90:10 dan 80:20 sebagai pengental pada tekstil printing menghasilkan produk akhir yang setara dengan pengental komersial manutex. 
Feasibility Study on the Profitability of Vannamei Shrimp Aquaculture on Coastal Area of Keburuhan Village, Purworejo Regency Djumanto, Djumanto; Ustadi, Ustadi; Rustadi, Rustadi; Triyatmo, Bambang
Aquacultura Indonesiana Vol 17, No 1 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.203 KB) | DOI: 10.21534/ai.v17i1.49

Abstract

Brackish water vannamei shrimp aquaculture in Keburuhan village of Purworejo Regency is one of the shrimp aquaculture activities developed since 2010. Shrimp aquaculture enteprises were done by local communities which use sandy land coated by plastic sheet with an area of 1000-4500 m2. Some farmers suffered losses due to lack of technical knowledge in shrimp aquaculture, environmental carrying capacity, ecological and economic feasibility. The aim of this study was to determine the general condition of vannamei shrimp aquaculture and shrimp farming feasibility. The study was conducted by field surveys, interviews and questionnaires to 30 farmers. The survey was conducted in May-June 2016 by purposive sampling of farmers who were members of a joint venture group of Minamulyo. The results showed shrimp pond size aquaculture area was varied, with a range of 1000-1500 m2, 1500-2000 m2, 2000-3000 m2 and 3000-4500 m2. An average fixed cost required for construction and operation of shrimp pond aquaculture was approximately IDR 6,620,934 to 12,606,666 million/plot/cycle shrimp production, while the variable costs of each cycle production ranged from IDR 45,876,733 to 78,861,666. The revenue and net profit varied according to pond size. The net gain for the smallest pond (1000-1500 m2) was IDR 48,702,332/plot/cycle, and the largest pond (3000-4500 m2) was IDR 58,131,666/plot/cycle. The shrimp farmers could recover the original investment incurred within a period of 4-6 months (one half cycles). Vannamei shrimp aquaculture in Keburuhan village of Purworejo regency brings a variety positive and negative impacts to the development of the economic, social and environment. Positive impact included increase of income and welfare of farmers, while the negative impact was a decline in the quality of the pond environment.
Utilization of Wastewater from Vannamei Shrimp Pond for Rearing Milkfish in Keburuhan Coast Purworejo Sub-District Djumanto, Djumanto; Ustadi, Ustadi; Rustadi, Rustadi; Triyatmo, Bambang
Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.185 KB) | DOI: 10.21534/ai.v19i1.48

Abstract

Brackish water vannamei shrimp in the village of Keburuhan, Ngombol District of Purworejo Regency is one of the shrimp aquaculture activities developed since 2010. Shrimp farming enteprises is done by local communities which use the sandy soil coated plastic sheet with an area of 1000-4500 m2. There is a positive impact by increasing the income and welfare of farmers, but give negative effects in decline of surrounding pond environment. The shrimp farmers mostly disposed of wastewater directly into the sewers or the surrounding area. The wastewater of vannamei shrimp culture contains of uneaten feed and plankton that can be utilized to grow milkfish. The aim of this study was to determine the growth and feeding preferences for milkfish culture in wastewater reservoir of vannamei culture. The study was conducted from January to April 2017 by rearing of milkfish in earthen ponds of shrimp culture in wastewater reservoir. Wastes consist of feces and uneaten feed during shrimp rearing were siphoned and flowed into ponds wastewater reservoir. Milkfish juvenile with body length ranges from 9.3 to 9.7 cm and weight ranges from 3.4 to 6.9 g was introduced into three earthen ponds of 600 m2 each, with density of 6 individual/m2 in triplicate for 70 days. During the study, milkfish juvenile were not fed, but obtained wastewater from shrimp culture ponds. The wastewater from the shrimp ponds was siphoned and piped into a waste reservoir ponds every 3 days until shrimp harvesting. Fish samples of each pond as much as 40 individuals were taken biweekly and then measured the length and weight. Fish stomach was dissected and then digestive tract was taken to observe the species of plankton consumed. The results showed that milkfish could grow well in wastewater reservoir of shrimp culture. The average daily growth rate of length and weight of milkfish reached approximately 0.11 cm/day and 0.34 g/day, respectively. The length weight relationship of milkfish showed allometric growth pattern. The food type found in the intestinal was dominated by phytoplankton. Feed was dominated by genera of phytoplankton, which reach more than 99%. The most dominant of phytoplankton were Scenedesmus sp. (34.7%), then Crucigenia sp. (20.1%), followed by Cyclotella sp. (14.6%), and Clamydomonas sp. (7.5%) next Microcystis sp. (6.7%), while the other genera less than 4.2%. Further research is needed to determine the optimum density of milkfish to utilize wastewater of shrimp culture.
Tempat Persarangan Lebah Hutan (Apis dorsata F.) di Pulau Sermata Lamerkabel, Jacobus S. A.; Masauna, Esther D; Utuwaly, Imas D; Jaya, Firman; Lastriyanto, Anang; Yunus, Mochammad; Batoro, Jati; Erwan, Erwan; Masyithoh, Dewi; Ustadi, Ustadi
JURNAL PERTANIAN KEPULAUAN Vol 8 No 1 (2024): Jurnal Pertanian Kepulauan
Publisher : Fakultas Pertanian, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jpk.2024.8.1.1

Abstract

The aim of the study was to determine the nesting sites of Apis dorsata (F) forest bees. the method used in this research was a descriptive survey. The results showed that trees and rock cliffs are nesting sites for forest bees on the island of Sermata. The Banyan tree is a tree that is preferred as a nesting place for forest bees (4 trees) in addition to Mango trees (2 trees), Salawaku (2 trees) , Kemiri (1 tree) , and Parna (1 tree). The height of the nesting places of forest bee colonies on trees and rock cliffs from the ground is at an altitude of 2.82 m to 27.40 m. The mango tree is the lowest forest bee nesting tree, while the highest is found in the banyan tree (20.51 m) and rock cliff 27.40 m. The habitat for the most forest bee colonies was 95 colonies in the range > 0-100 masl and 20 colonies in the range > 100-200 masl. The number of forest bee nests found in the locations of the villages of Regoha, Rotnama, Lelang, and Elo was 115 colony nests.
PENGARUH pH, SUHU DAN JENIS SUBSTRAT TERHADAP AKTIVITAS KITINASE Bacillus sp. RNT9 Pamungkas, Satrio Adil; Puspita, Indun Dewi; Ustadi, Ustadi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 19, No 1 (2023): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.19.1.29-39

Abstract

Limbah perikanan yang berasal dari udang, kepiting, dan kerang, umumnya mengandung kitin yang merupakan suatu polisakarida. Kitin memiliki struktur polimer linier yang terdiri dari monomer β-1,4-N-asetil-D-glukosamin, dan memiliki banyak manfaat dalam bentuk produk turunannya. Produk turunan kitin, seperti glukosamin dan N-Asetilglukosamin, memiliki nilai ekonomi yang tinggi sebagai bahan baku alternatif di berbagai industri seperti farmasi dan pangan. Proses degradasi kitin menjadi senyawa tersebut dapat dilakukan melalui reaksi enzimatis dengan bantuan enzim kitinase yang diproduksi oleh bakteri. Penelitian ini bertujuan untuk mengetahui pengaruh pH (6, 7 dan 8), suhu (30ºC, 35ºC dan 40ºC) dan jenis substrat (koloidal kitin, kitin serbuk dan tepung cangkang udang) terhadap aktivitas kitinase Bacillus sp. RNT9. Parameter yang diuji adalah aktivitas kitinase (U/mL) dan kadar N-Asetilglukosamin (NAG) medium (ppm). Kedua parameter ini diukur secara kuantitatif dengan metode kolorimetri. Hasil penelitian menunjukan bahwa kondisi terbaik untuk menghasilkan aktivitas kitinase pada Bacillus sp. RNT9 adalah dengan medium pH 8, suhu inkubasi 35ºC dan jenis substrat kitin koloidal. Aktivitas kitinase tertinggi yang diproduksi Bacillus sp. RNT9 berturut-turut sebesar 0,0008 U/mL pada perlakuan pH 8 pada hari ke-2 fermentasi, 0,0013 U/mL yang diperoleh pada perlakuan suhu 35ºC hari untuk ke-4 dan 0,0010 U/mL yang diperoleh pada perlakuan jenis substrat koloidal kitin untuk hari ke-2 fermentasi. Konsentrasi NAG mencapai nilai tertinggi pada optimasi pH 8 untuk hari ke-2 fermentasi sebesar 9,5968 ppm. Pada optimasi suhu 35ºC untuk hari ke-4 fermentasi sebesar 32,387 ppm serta pada perlakuan optimasi jenis substrat koloidal kitin untuk hari ke-4 fermentasi sebesar 26,031 ppm. Fishery waste originating from shrimp, crabs and clams, generally contains chitin which is a polysaccharide. Chitin has a linear polymer structure consisting of β-1,4-N-acetyl-D-glucosamine monomers, and has many benefits in the form of its derivative products. Chitin derivative products, such as glucosamine and N-acetylglucosamine, have high economic value as alternative raw materials in various industries such as pharmaceuticals and food. The process of degradation of chitin into these compounds can be carried out through enzymatic reactions with the help of chitinase enzymes produced by bacteria. This study aims to determine the effect of pH (6, 7 and 8), temperature (30ºC, 35ºC and 40ºC) and type of substrate (colloidal chitin, chitin powder and shrimp shell flour) on the chitinase activity of Bacillus sp. RNT9. The parameters tested were chitinase activity (U/mL) and medium N-Acetylglucosamine (NAG) levels (ppm). Both of these parameters were measured quantitatively by the colorimetric method. The results showed that the best conditions for producing chitinase activity in Bacillus sp. RNT9 is with a medium of pH 8, incubation temperature of 35ºC and the type of substrate is colloidal chitin. The highest chitinase activity produced by Bacillus sp. RNT9 was 0,0008 U/mL, respectively, at pH 8 treatment on the 2nd day of fermentation, 0,0013 U/mL obtained at 35ºC temperature treatment for the 4th day and 0,0010 U/mL obtained at treatment type of chitin colloidal substrate for the 2nd day of fermentation. The concentration of NAG reached the highest value at the optimization of pH 8 for the 2nd day of fermentation of 9,5968 ppm. At the optimization temperature of 35ºC for the 4th day of fermentation it was 32,387 ppm and at the optimization treatment of chitin colloidal substrate for the 4th day of fermentation it was 26,031 ppm.
Manajemen Pembelajaran Guru Dalam Meningkatkan Hasil Belajar Siswa Selama Pandemi Covid-19 Ustadi, Ustadi; ,, Barnoto; Qutsiyah, Dewi Afiatul
Chalim Journal of Teaching and Learning Vol. 2 No. 1 (2022): Islamic Education
Publisher : Program S3 Pendidikan Islam Institut Pesantren KH. Abdul Chalim Pacet Mojokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31538/cjotl.v2i1.221

Abstract

With the outbreak of the covid-19 pandemic, social dynamics are changing rapidly, including in the world of education. Where the transition of learning that was previously carried out face-to-face has shifted to a virtual space. The purpose of this study is to find out and analyze the planning, implementation and evaluation of online learning management at SDN Sawahan Mojosari during the pandemic.The type of research in this research is qualitative with a case study approach. Data collection techniques by means of observation, interviews and documentation. analysis using data reduction methods, data presentation and drawing conclusions. Techniques for data validity are credibility, transferability, dependability, and confirmability. The research results are. 1). The planning stage is carried out through several stages, namely first a small meeting of the principal with the school committee, as well as teachers, then the results of the meeting are socialized to parents and students 2). The implementation of online learning during the COVID- 19 pandemic at SDN Sawahan Mojosari was handed over to the implementation by the principal to each class teacher, namely by using the Whatsapp group Mapel, Google Classroom and Google Form to send materials, be it photos, videos, or LKPD 3). Evaluation of online learning during the COVID-19 pandemic at SDN Sawahan Mojosari was carried out by the Principal together with class teachers directly into the class group or through the teacher's personal WA
Influence of Enzyme Concentration, Hydrolysis Duration, and Drying Temperature on the Production of Antioxidant-containing Peptide from Catfish (Clarias sp.) Gills Prima Putra, Masagus Muhammad; Wisnumurti, Winnen; Athallah, Nezar Helmi; Zaen, Al Tarich Arasy; Sahura, Aulia Rachma; Ustadi, Ustadi
Journal of Applied Food Technology Vol 12, No 1 (2025)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.26160

Abstract

Catfish gills are a potential raw material for producing protein hydrolysate with antioxidant activity. Protein hydrolysate was produced using crude papain enzyme in two stages. The first stage was to obtain the optimum enzyme concentration (w/w) including 1%, 2%, 3%, 4%, 5% and 6%, with 0% as a control, followed by the second stage to obtain the optimum hydrolysis duration for 24, 48, 72, and 96 hours, with 0 hour as a control. The supernatant containing protein hydrolysate was then dried using an oven at various temperatures, including 60, 70, and 80°C, for 48 hours to obtain catfish gill protein hydrolysate (CGH) powder. CGH was tested for degree of hydrolysis (DH) and antioxidants, including DPPH and ABTS. The highest antioxidant activity was obtained from 3% papain with DPPH and ABTS values of 85% and 9.57 µM TEAC, respectively. Further stage on the hydrolysis duration gave 48 hours as the optimal one, with antioxidant activity of 85.25 % for DPPH and 4.29 µM TEAC for ABTS. The oven-drying temperature concluded that CGH has stable antioxidant activity. The IC50 on antioxidant activity based on DPPH ranged from 1799.85 mg/L to 1749.50 mg/L (IC50 of ascorbic acid was 2.61 mg/L) and was included as very weak. Even though based on IC50, CGH has low antioxidant activity, the protein content was found to be high (56.86±1.51%), which could be a high-protein food additive.
Co-Authors ,, Barnoto Amir Husni Anang Lastriyanto Athallah, Nezar Helmi Bambang Triyatmo Djumanto Djumanto Erwan Erwan, Erwan Firman Jaya Jati Batoro Lamerkabel, J. S. A. Masauna, Esther D Masyithoh, Dewi Mochammad Yunus, Mochammad Pamungkas, Satrio Adil Prima Putra, Masagus Muhammad Puspita, Indun Dewi Qutsiyah, Dewi Afiatul Riky Paskandani, Riky Rustadi Rustadi Sahura, Aulia Rachma Subaryono Subaryono, Subaryono Tazwir Tazwir Utuwaly, Imas D Wisnumurti, Winnen Yudi Pranoto Zaen, Al Tarich Arasy