Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Jurnal Veteriner
Agus Purnomo
Akademi Peternakan Brahmaputra Yogyakarta
Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah Khusnan Khusnan; Agus Purnomo
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.451 KB) | DOI: 10.19087/jveteriner.2022.23.1.42

Abstract

Susu sapi merupakan media pertumbuhan bakteri patogen maupun non patogen. Escherichia coli mrupakan salah satu bakteri yang sering ditemukan pada susu sapi mentah. Escherichia coli patogen pada sapi dapat menyebabkan mastitis maupun radang usus serta dapat menyebabkan masalah kesehatan pada manusia seperti diare, kolitis hemoragik dan sindrom uraemik hemolitik. Hemaglutinin dan hemolisin merupakan faktor virulen yang penting pada E. coli patogen, karena berperan sebagai faktor adhesin, dan faktor pertahanan terhadap fagositosis. Tujuan penelitian ini mendeteksi hemaglutinin dan hemolisin E. coli isolat asal susu sapi mentah serta kemampuan adhesi pada sel epitel serta pertahanan terhadap fagosit netrofil. Hasil penelitian ini menunjukkan 100% isolat E. coli tidak memiliki hemaglutinin dan 17,2% merupakan E. coli hemolitik. Pada uji adhesi mampu melekat pada sel epitel bukalis 46,87 bakteri/sel. Pada uji fagositosis isolat-isolat hemolitik lebih sedikit difagosit oleh neutrofil dibandingkan isolat non hemolitik (2,64 dibanding 3,1 bakteri) tiap neutrofil (P<0,05). Ditemukannya E. coli patogen pada susu mentah menegaskan pentingnya pasteurisasi pada susu sebelum dikonsumsi.
Multidrug-Resistant Strain ExPEC Isolat Asal Puyuh (Coturnix coturnix japonica) Wahyu Prihtiyantoro; Agus Purnomo; Sudarisman .; Khusnan .
Jurnal Sain Veteriner Vol 41, No 2 (2023): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.78592

Abstract

AbstractExtraintestinal Escherichia coli (ExPEC) causes colibacillosis in poultry, including quail. ExPEC is a pathogenic E. coli that causes colibacillosis outside the gastrointestinal tract of poultry, in the form of peritonitis, pericarditis, salpingitis, synovitis, osteomyelitis, septicemia, pneumonitis, nephritis, pleurisy, proventriculitis and ventriculitis. In poultry farming antibiotics are used to promote growth and egg production, prevention and treatment of bacterial infections. Antibiotic resistance is a new problem that arises in poultry farming. In this study, 24 isolates of ExPEC strain from quail were used. These isolates did not differentiate sorbitol in the MacConkey (SMAC) sorbitol test assay. Antibiotic resistance was tested based on the inhibition of bacterial growth on Muller Hinnton Agar (MHA) media. 10 types of antibiotics were used to observe their resistance in all isolates. Resistance to Amikacin (87.5%), Ampicillin (87.5%), Ciprofloxacin (100%), Clindamycin (95.83%), Cefoxacin (25.0%), Doxycycline (87.5%), Erythromycin (100%), Gentamicin (95.83%), Penicillin (95.83%) and Tetracycline (83.3%). The isolates had multidrug-resistance between 3 to 10 types of antibiotics. This study showed that ExPEC isolates from quail were resistant to the type of antibiotics tested, so that the use of antibiotics in quail culture should be limited to reduce and prevent the emergence of new antibiotic resistance. Keyword: Escherichia coli, sorbitol-negative, quail, antibiotics resistance
Co-Authors Khusnan . Khusnan k Sudarisman . Wahyu Prihtiyantoro