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PROTOTIPE KTT DIAGNOSTIK UNTUK DETEKSI STREPTOKOKOSIS PADA PRIMATA DENGAN ELIS A-ANTIBODI MONOKLONAL PENANGKAP ANTIGEN Siti Isrina Oktavia Salasia; Aris Purwantoro; . Khusnan
Jurnal Sain Veteriner Vol 25, No 1 (2007): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan mengembangkan sarana diagnostik untuk kontrol streptococcosis pada primata dengan ELISA-monoclonal antibody penangkap antigen M-like protein (MLP) Streptococcus equi subsp. zooepidemiats grup C (SGC). M-like protein SGC diekstraksi dengan menggunakan lisozim dan Nasetilmuramidase. Protein dengan besar sekitar 58 kDa digunakan sebagai antigen untuk menimbulkan antibodi pada mencit Balb/c. Mencit yang mengandung antibodi dengan absorban tertinggi (2,868) diambil limpanya untuk memperolehl imfosit imUn( limfoblast). Hasil fusi sel mieloma dan limfoblast diperoleh4 klon hibridoma yang positif mengandung antibodi terhadap MLP, dengan nilai absorban pada ELISA masing-masing 1,900, 1,963, 1,895 dan 2,050. Hasil propagasi cairan asites mencit Balb/c diperoleh monoklonal antibodi terhadapMLP S. equi subsp. zooepidemicus dengan nilai absorban dan konsentrasi sebagai berikut: asitesI = 1,597(5,50mg), asites 2 : 1,940 (5,75 mg), dan asites 3 : 3,012 (5,80 mg). Antibodi monoklonal memperlihatkanspesifitas yang cukup tinggi karena hanya mengenal I epitop spesifik yang diperlihatkan pada uji Western blot dengan menampakkan pita tunggal pada sekitar 58 kDa dan menunjukkan reaksi positif pada uji dot-blot. Antibodi monoklonal memperlihatkan sensitifitas yang cukup tinggi setelah diuji dengan serum hewan percobaan tikus yang diinfeksi buatan dengan S. equi subsp. zooepidemicus dengan hasil absorban pada uji ELISA lebih dari 1,00 dan menunjukkan reaksi positif pada uji dot-blot. Hasil uji terhadap sampel serum Macacafascicularis menunjukkan bahwa 97 ,56% positif.Kata kunci: S. equi subsp. zooepidemicus, antibodi monoklonal, M-like protein, primata

Respon Neutrofil, Adesi Pada Sel Epitel, Aglutinasi Eritrosit Terhadap Staphylococcus aureus : Kajian Hidrofobisitas In Vitro = Response of neutrophils, epithelial cells adhesion, erythrocytes agglutination of Staphyloco Khusnan .; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus merupakan salah satu bakteri potensial sebagai penyebab utama mastitis pada sapi perah. Mastitis dapat menyebabkan kerugian ekonomi peternak akibat turunnya produksi susu. Infeksi bakteri dapat terjadi melalui kemampuan bakteri memasuki hospes, berkembang biak, merusak jaringan inang dan mampu bertahan dalam tubuh hospes. Penelitian ini bertujuan untuk mengetahui hubungan sifat hidrofobisitas S. aureus dan kemampuannya terhadap aglutinasi eritrosit, pelekatan dengan sel epitel dan kemampuan bertahan terhadap fagositosis sel polimorfonuklear. Dari 10 isolat S. aureus yang digunakan dalam penelitian ini, terdapat 8 isolat bersifat hidrofob dan 2 isolat bersifat hidrofil. Diantara isolat yang bersifat hidrofob terdapat 2 isolat mempunyai kemampuan mengaglutinasi eritrosit sapi perah, kambing, domba. Staphylococcus aureus yang bersifat hidrofob dan hemaglutinasi positif, lebih banyak melekat pada sel-sel epitel bukalis dan lebih banyak difagosit oleh sel-sel PMN dibanding isolat yang bersifat hidrofob tetapi hemaglutinasi negatif maupun isolat yang bersifat hidrofil. Isolat yang bersifat hidrofil tidak mampu mengaglutinasi eritrosit dan lebih sedikit melekat pada sel-sel epitel dan lebih sedikit difagosit oleh sel-sel PMN.

KARAKTERISASI FENOTIPE Streptococcus suis = THE PHENOTYPE CHARACTERIZATION OF Streptococcus suis Khusnan .; Siti Isrina Oktavia S.
Jurnal Sain Veteriner Vol 19, No 2 (2001): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.381

Streptococcus suis telah diketahui menyebabkan meningitis, artritis dan septikemia pada babi, manusia dan hewanhewan lain. Penelitian ini bertujuan untuk mengetahui sifat fenotipe Streptococcus sins rneliputi sifat pertumbuhannyi dalam media cair, media soft agar, morfologi koloni dan sifat pelekatannya dalam uji heksadekan. Dari 34 isolat Streptococcus suis yang digunakan dalam penelitian ini terdapat 5 isolat tumbuh jernih dalam media cair dengan sedimen tampalc pada dasar tabung, tumbuh kompak dalam media soft agar, dengan pewarnaan methylene blue koloni membentuk rantai panjang dan berdasar uji heksadekan bersifat hidrofob. Kultur Streptococcus suis yang lain (29 isolat) tumbuh keruh dalam media cair, tumbuh difus dalam media soft agar, membentuk koloni tunggal atau rantai pendek dan bersifat hidrofil.

Karakterisasi Faktor-faktor Virulensi Staphylococcus aureus Asal Susu Kambing Peranakan Ettawa secara Fenotip dan Genotip Khusnan Khusnan; Wahyu Prihtiyantoro; Hartatik Hartatik; Mitra Slipranata
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is a major cause of mastitis in large or small ruminants, and often manifested by subclinical mastitis in Peranakan Ettawa (PE) goats. Staphylococcus aureus in human can cause food borne disease. The research aimed to characterize the virulence factors of Staphylococcus aureus isolated from milk PE goats, phenotypic- and genotypically. Phenotypically characterization were determined through the pigmen assay as well as hydrophobicity, haemolysin, and hemaglutinin reaction. Polymerase chain reaction (PCR) analysis was used to detect 4 virulen genes including coa, clf, fnbA, and fnbB genes. The results of research showed that Staphylococcus aureus abled to produce white pigmen (35,7%), yellow pigmen g (57,1%), andorange pigmen (7,2%). Staphylococcus aureus showed α-hemolysis zone (35,7%), β-hemolysis (35,7%), dan γ-hemolysis (28,9%). Hydrophobicytic test revealed 14,3% Staphylococcus aureus isolates were hydrophobe and85,7% hydrophil. Staphylococcus aureus (85,7%) isolates abled to aglutinated sheep blood cells. Based on genotypic analysis of Staphylococcus aureus could be detected coa gene (92,8%), clf gene (64,3%), fnbA gene (78,6%), and fnbB gene (64,3%). Based on the phenotypic and genotypic characters, it can be concluded that Staphylococcus aureus are virulent strains. This information can be used as the basis for control mastitis in PE goats

Deteksi Hemaglutinin, Hemolisin dan Koagulase Secara Fenotipik dan Genotipik pada Staphylococcus aureus Isolat Asal Broiler Khusnan Khusnan; Dwi Kusmanto
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is a bacterium causing diseases in animals and human. Staphylococcus aureus in broilers cause septicemia, tendosinovitis, dermatitis, endocarditis, wound infections, arthritis and bumblefoot. In this research, 24 isolates of Staphylococcus aureus from broiler were characterized of its virulent factors including the presence of haemagglutinin, ability to agulate the plasma in tubular coagulase test as well presipitate formation in clumping factor test, and haemolysis types. By polymerase chain reaction (PCR) were genotypically detected genes coa, clf, hlaA, and hlaB. All of the isolates (100%) had haemagglutinin, capable to agglutinate and precipitae of rabbit plasma. All isolates could lyse sheep red blood cells with the type of α-hemolysis (45.8%), β-hemolysis (50.0%) and γ-haemolysis (4.2%). Genotypically, all isolates (100%) had coa and clf genes, hlaA gene 7(0.8%) and hlaB gene (29.2%).

Prevalensi Strain Avian Pathogenic Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh Wahyu Prihtiyantoro; Khusnan Khusnan; Mitra Slipranata; Imron Rosyidi
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Avian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. Polymerase chain reaction (PCR) was used to analyse genetopically to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. The results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. As a zoonotic agent, the isolates have an impact on human health.

Peran Hemaglutinin dan Hemolisin pada Escherichia coli Sorbitol-negatif Isolat Burung Puyuh pada Proses Infeksi Secara in Vitro Khusnan Khusnan; Wahyu Prihtiyantoro; Dwi Kusmanto
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69896

Abstract Escherichia coli sorbitol-negative in quails cause economic loss due to the death, growth rate inhibition, decreased egg production, and increased medical treatment. Escherichia coli sorbitol-negative has many virulence factors, including hemagglutinin and hemolysin. The aim of this study is to determine the role of hemagglutinin and hemolysin in the infection process of Escherichia coli sorbitol-negative in vitro. This study was performed using 23 isolates of Escherichia coli sorbitol-negative from quails, 52.2% (12 of 23 isolates) had hemagglutinin, while 34.8% (8 out of 23 isolates) had hemolysin. Isolates with hemagglutinin were more attached to human buccal epithelial cells than isolates without hemagglutinin (P <0.05). Isolates with hemolysin were less phagocyted by macrophages compared to isolates which without hemolysin (P <0.05). Escherichia coli sorbitol-negative isolates from quails that have hemagglutinin and hemolysin are pathogenic isolates that possess the potential to cause colibasilosis and transmission between quails and other birds.

KARAKTERISASI FAKTOR VIRULENSI Escherichia coli PATOGEN ZOONOTIK (O157:H7) ISOLAT ASAL TINJA SAPI POTONG CHARACTERIZATION OF Escherichia coli VIRULENCE FACTORS OF ZOONOTIC PATHOGENS (O157:H7) ISOLATE OF FECAL CATTLE Wahyu Prihtiyantoro; Hartatik Hartatik; Khusnan Khusnan; Mitra Slipranata; Fatkhanudin Aziz
Agros Journal of Agriculture Science Vol 16, No 2: Edisi Juli 2014
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.171 KB)

Kemampuan hemaglutinasi, keberadan hemolisin dan kekebalan terhadap antibiotik merupakan faktor virulensi yang penting pada Escherichia coli. Penelitian ini bertujuan melakukan karakterisasi faktor virulensi terhadap 9 (sembilan) E. coli isolat sapi potong yang terdiri 5 (lima) isolat berasal dari tinja dan 4 (empat) isolat berasal dari pupuk kandang. Duapuluh dua persen isolat mampu menggumpalkan eritrosit dan 100 persen isolat tidak memiliki hemolisin, 4 isolat asal tinja positif sebagai patogen zoonotik (O157:H7). Kekebalan isolat oleh eritromisin, metisilin, penisilin, tetrasiklin dan gentamisin masing-masing 66,7 persen, 66,7 persen, 66,7 persen, 16,7 persen dan 0 persen. Resistensi hanya terjadi pada isolat asal tinja sedangkan isolat asal pupuk kandang masih sensitive terhadap antibiotika-antibiotika tersebut. Hemagglutination ability, the existence of hemolysin and antibiotic resistance is an important virulence factor in Escherichia coli. This study aimed to characterize the virulence factors of the nine (9) E. coli isolates beef cattle comprising five (5) isolates from feces and 4 (four) isolates from manure. Twenty-two percent of isolates were able to aglutinate erythrocytes and 100 percent of isolates did not have hemolysin. Immunity isolates by erythromycin, methicillin, penicillin, tetracycline and gentamicin respectively 66.7 percent, 66.7 percent, 66.7 percent, 16.7 percent and 0 percent. Resistance occurs only at the fecal isolates while isolate of manure is still sensitive to the antibiotics

KEMAMPUAN ADHESI Escherichia coli HEMAGLUTINASI DAN SMAC POSITIF ISOLAT SAPI POTONG PADA SEL EPITEL BUKALIS SECARA IN VITRO Wahyu Prihtiyantoro; Hartatik Hartatik; Khusnan Khusnan
Agros Journal of Agriculture Science Vol 18, No 2: Edisi Juli 2016
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.338 KB)

Adhesi bakteri pada permukaan sel epitel inang merupakan awal dari proses infeksi. Bakteri yang mempunyai faktor-faktor virulensi dan faktor adhesin lebih mudah beradhesi pada sel epitel. Penelitian ini bertujuan untuk mengetahui kemampuan adhesi isolat-isolat Escherichia coli yang memiliki hemaglutinin dan strain Escherichia coli zoonotik pathogenic (VTEC). Isolat-isolat yang memiliki hemaglutinin dan strain VTEC memiliki kemampuan adhesi yang lebih tinggi dibandingkan dengan isolat-isolat yang tidak memiliki hemaglutinin dan strain non VTEC.

RESISTENSI ANTIBIOTIK DAN DETEKSI GEN PENGODE METHICILLIN RESISTANT Staphylococcus aureus (MRSA) ISOLAT BROILER DI WILAYAH YOGYAKARTA (Resistance of Antibiotics and Detection of Gene Encoding Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Broiler in Yogyakarta) Khusnan K; Dwi Kusmanto; Mitra Slipranata
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The aims of the research were to evaluate resistance of Staphylococcus aureus against several antibiotics and to detect the gene encoding ofmethicillin resistant Staphylococcus aureus (MRSA) in broiler. Twenty three Staphylococcus aureus isolated from infected broiler in Yogyakartawere used. Genotypic identifications were done based on the occurrence of gene nuc and 23SrRNA. The resistance assay to several antibioticsrevealed that Staphylococcus aureus were resistant to penicillin 78% (18/23), doxycyclin 56% (15/23), gentamicin 26% (6/23), tetracyclin 22% (5/23), erythromycin 13% (3/23), and methicillin 9% (2/23). Detection of gene encoding MRSA on 23 isolats of S. aureus showed that 8 (34.8%) isolates contain gene mecA.Key words: Staphylococcus aureus, broiler, resistant, methicillin, mecA

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