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All Journal BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) Jurnal Biogenerasi KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pengabdian Masyarakat
Zahratul Idami
Universitas Islam Negeri Sumatera Utara
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Other

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UJI MOLEKULER MENGGUNAKAN GEN rbcL PADA TUMBUHAN PEGAGAN (Centella asiatica (L.) Urban) Daffa Rizki Syahreza; Efrida Pima Sari Tambunan; Zahratul Idami
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.8055

Abstract

Uji secara molekuler dilakukan dengan menentukan satu penanda yang tepat untuk menganalisis beberapa kajian dalam bidang molekuler. Uji secara molekuler tumbuhan dilakukan menggunakan penanda gen rbcL (Ribulose-1,5-biphosphate carboxylase/oxygenase large subunit gene). Tumbuhan pegagan (Centella asatica (L.) Urban) merupakan herba yang pada umumnya hidup secara liar di tempat terbuka seperti padang rumput, kebun, tepi jalan, dan tepi parit. Tempat tumbuh pegagan seperti dataran tinggi, dataran rendah, dan pesisir pantai sangat mempengaruhi perubahan dari segi molekuler. Tujuan penelitian ini untuk mengetahui bagaimana karakteristik molekuler, keragaman molekuler (keragaman genetik) dan hubungan filogenetik tumbuhan pegagan (Centella asiatica (L.) Urban) dengan menggunakan gen rbcL. Penelitian ini memiliki tahapan yaitu koleksi sampel, isolasi DNA, amplifikasi DNA dengan gen rbcL, elektroforesis, sekuensing, dan analisis data dengan MEGA 11. Hasil penelitian dari amplifikasi menggunakan gen rbcL pada DNA genom Centella asiatica (L.) Urban yang diteliti menghasilkan panjang amplikon kurang lebih 600 bp. Pada penyatuan sekuens yang konsesus pada sampel mendapatkan sekuens dengan panjang D1 567 bp dan D2 562 bp. Pembentukan pohon filogenetik menggunakan metode Neighbor-Joining dan model perhitungan Kimura-2-Parameter menunjukkan Centella asatica (L.) Urban termasuk dalam kelompok monofiletik. Hasil analisis keragaman molekuler Centella asatica (L.) Urban menunjukkan bahwa pada seluruh sampel yang dikoleksi dari Desa Pantai Labu Pekan Kabupaten Deli Serdang dan Jl. Air Bersih Ujung Kota Medan memiliki keragaman molekuler. Maka dari itu, dapat disimpulkan bahwa gen rbcL menghasilkan barcode DNA dapat digunakan sebagai metode untuk mengidentifikasi Centella asatica (L.) Urban secara molekuler dan sangat efisien dalam menentukan hubungan kekerabatan dengan spesies yang lainnya.
Moringa Leaf Extract on Changes in CD36 mRNA Gene Expression in the Brain of Rats Exposed to Ethylene Glycol Gilang Wirayudha; Zahratul Idami
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 9 No. 1 (2026): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/yjpg5h56

Abstract

This study aimed to analyze the effect of Moringa oleifera administration on CD36 mRNA expression in rat tissues exposed to ethylene glycol. The method used was an experimental design with gene expression analysis performed using RT-PCR and GAPDH as an internal control gene. The results showed that CD36 mRNA expression increased in the ethylene glycol-exposed group compared to the normal group. In the treatment groups, different doses of Moringa oleifera produced varied expression responses, where a certain dose reduced gene expression toward normal conditions, while a higher dose increased the expression again. The expression of GAPDH remained relatively stable across all groups, confirming its suitability as a reference gene. In conclusion, Moringa oleifera administration affects CD36 mRNA expression in a dose-dependent manner and has the potential to attenuate the toxic effects of ethylene glycol at specific doses. Keywords: Ethylene Glycol, Moringa Leaf, CD36 mRNA Expression
Pendampingan Partisipatif Digital Marketing pada UMKM Keripik Berbasis Biopreneur di Desa Celawan sebagai Upaya Optimalisasi Potensi Ekonomi Desa Rifaldy Yusliandi; Bunga Sausan Sabilla; Herly Febri Fathiha; Nur Azmi Rahmadhini; Zahratul Idami
Jurnal Pengabdian Masyarakat - PIMAS Vol. 5 No. 1 (2026): Februari
Publisher : LPPM Universitas Harapan Bangsa Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35960/pimas.v5i1.2140

Abstract

Biopreneur-based MSME chip producers in Celawan Village utilize local food resources such as bananas and cassava as their main sources of production, but their economic potential has not been optimized due to weak business identity, low digital marketing literacy, and limited market access. This community service activity aims to describe participatory digital marketing assistance as an effort to optimize the economic potential of the village by strengthening bio-entrepreneur-based MSME chips. The method used is a participatory approach through field observation, direct assistance, and contextual education according to the needs of MSME actors. The assistance focuses on creating a business brand, improving hygienic food product packaging, digitizing business locations through Google Maps, and utilizing simple digital media as a marketing tool. The results of the activity showed an increase in MSME actors' awareness of the importance of business identity, product quality, and digital marketing in increasing the added value of local food products. This mentoring was considered capable of expanding business visibility and supporting the optimization of village economic potential in a sustainable manner.
Periphyton Community Structure on Natural and Artificial Substrates in the Namu Sira-Sira River, Langkat Regency, North Sumatra Fathia Azzahra; Melfa Aisyah Hutasuhut; Zahratul Idami
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6862

Abstract

Periphyton is one of the biotic components, living attached to various substrates has an important role in providing primary productivity of waters. The purpose of this study was to determine the identification of species, the structure of the periphyton community in natural and artificial substrates, the water quality of the Namu Sira-sira River in Langkat Regency. The data collection method was carried out using the "Purposive Random Sampling" method at three stations according to community activities in the research area.The results of the study obtained species found in natural substrates were 25 species and artificial substrates were 27 species. The community structure including the diversity index (H ') is included in the medium category with a value of 2.48 on natural substrates and artificial substrates with a value of 2.50. The uniformity index value (E) on natural substrates is 0.89 and artificial substrates are 0.90 categorized as high because the distribution of species in each station is even. Periphyton communities on natural substrates have advantages in terms of abundance and diversity compared to artificial substrates due to the complex interactions between environmental factors, substrate quality, and ecological processes that support their growth
IDENTIFIKASI KERAGAMAN MORFOLOGI DAUN TANAMAN UBI KAYU (Manihot escolenta Cantz) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Hafiza Hazmi; Zahratul Idami; Kartika Manalu
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/87gabb18

Abstract

Cassava Plants (Manihot esculenta Crantz), traditionally cultivated and offering many benefits as food ingredients, animal feed, and industrial raw materials. Cassava is also known to be rich in carbohydrates, soluble dietary fiber, and beneficial micronutrients for the body. Morphological and genetic diversity in cassava plants is crucial for the development of superior and improved varieties. This study aims to identify the morphological diversity of cassava leaf plants (Manihot esculenta Crantz) and analyze dendrogram relationships using RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in Serdang Bedagai Regency and the laboratory of the State Islamic University of North Sumatra, Medan. The methods used included sample collection, DNA isolation, PCR amplification, electrophoresis, and data analysis. The results showed variations in leaf morphology as observed in the number of leaflets, color, and shape of leaves in cassava varieties Genderuwo, Roti, Keriting, and Jari. Morphologically, the Genderuwo and Roti cassava leaves were the most similar. The dendrogram analysis using RAPD primers OPA 2, OPA 3, OPA 5, and OPA 7 showed a close relationship between Genderuwo and Roti varieties (coefficient 0.85), while the Jari variety had the most distant relationship (coefficient 0.60). This research can serve as a reference in cassava plant breeding to produce superior varieties  
ANALISIS FINGERPRINT DNA GENOTIPE BELIMBING DENGAN GENUS Averrhoa MENGGUNAKAN PRIMER RANDOM AMPLIFIED POLYMORPHIC Nur Hikmah; Zahratul Idami; Kartika Manalu
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/5d69v436

Abstract

Fingerprinting is a technique in DNA technology that can be used to see individual diversity and can also differentiate individuals from each other even though they are very closely related. This study aims to see the results of DNA fingerprinting of starfruit genotypes with the genus Averrhoa using Random Amplified Polymorphic DNA (RAPD) primers and to see the polymorphism value of each Random Amplified Polymorphic DNA (RAPD) primer. The research method used is Fingerprint DNA with Random Amplified Polymorphic DNA (RAPD) primers, namely OPA-2, OPA-3, OPA-5, OPA-7, OPD-11 and OPD-13. The results of the research show that the fingerprint results can be seen clearly. using electrophoresis. The best results were the primers OPA 2, OPA3 with 6 band patterns, followed by the second highest number of OPA5, OPD 11 with 5 band patterns, and the third highest number of OPA7, OPD 13 with 4 DNA fingerprint band patterns, the results of the kinship relationship between each Averrhoa have The polymorphic value shows that sweet starfruit (Averrhoa carambola L.) has a relationship of around 0.62% with starfruit. wuluh ( Averrhoa bilimbi L.). The Demak starfruit (Averrhoa carambola Cultivar Demak) has a relationship of around 0.71% with the Sembiring starfruit (Averrhoa carambola).  
ANALISIS KERAGAMANGENETIK DNA UBI JALAR (Ipomea batatas L.)DENGAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)   Tri Wulan Dari; Zahratul Idami; Kartika Manalu
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/2dg8y347

Abstract

Sweet potato (Ipomea batatas L.) is a national food security supported by one of the potential plant resources. After rice, corn, and cassava, sweet potato (Ipomea batatas L.) contains the fourth highest carbohydrate. Sweet potato is a good source of thiamine (vitamin B1), riboflavin, vitamin A, vitamin B, and vitamin C. The purpose of this study was to determine the genetics of the analysis results presented in sweet potato (Ipomea batatas L.) using dendograms and DNA banding patterns in sweet potatoes based on RAPD markers. The research method used is an experimental method to collect data by conducting direct experiments, isolating DNA, and conducting PCR using the RAPD technique; Six RAPD primers were used: OPA 2, OPA 3, OPA5, OPA7, OPD 11, and OPD 13. The study resulted in genetic diversity seen from the DNA banding pattern that forms polymorphism. RAPD produces bands measuring 100-900 bp. The genetic diversity of 4 types of Ipomea batatas L. plants analyzed using the NTSYS pc-2.02i application on the kinship relationship displayed with a dendogram shows the same diversity, namely orange sweet potatoes with yellow sweet potatoes, which have the highest level of similarity because they join at a coefficient value of around 0.91. While in purple sweet potatoes joining the group (yellow sweet potatoes and orange sweet potatoes) at a coefficient value of 0.86 indicates that purple sweet potatoes are slightly more different but still in the group. Then in white sweet potatoes have a connection coefficient value of around 0.81 which indicates that this sample is the most different compared to the others)
Co-Authors Bunga Sausan Sabilla Daffa Rizki Syahreza Efrida Pima Sari Tambunan Eka Nuryani Fathia Azzahra Gilang Wirayudha Hafiza Hazmi Hamidah Harahap Herly Febri Fathiha Ike Ramayati Ikhlasul Janu Nst Julreda Banu Br Sitorus Kartika Manalu Melfa Aisyah Hutasuhut Nur Azmi Rahmadhini Nur Hikmah Revi Lestari Rifaldy Yusliandi Rizki Amelia Nasution Tri Wulan Dari Wira Hariati Simbolon