Sulistiawati Sulistiawati
Department Of Periodontics, Dentistry Study Program, Faculty Of Medicine Universitas Sriwijaya

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THE EFFECT OF SEMENDO COFFEE ON THE NUMBER OF FIBROBLAST CELLS IN GINGIVA WOUND OF RATS Sulistiawati Sulistiawati; Hema Awalia; Ulfa Yasmin; Rosada Sintya Dwi; Alya Namira
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 9, No 1 (2022)
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v9i1.932

Abstract

Introduction: Gingiva is a keratinized epithelium tissue that surrounds the tooth and protects the tissue beneath it, which is often wounded. Sumatran robusta coffee, semendo coffee, consists of active compounds such as polyphenol, alkaloid, and saponin that can increase fibroblast amount during the wound healing process. This study aims to determine the effect of semendo coffee (Coffea canephora) extract on fibroblast amount on gingiva wound of Rattus norvegicus. Methods: The true experimental study with a post-test-only control group design was confirmed in this study. This study used twenty-four rats (Rattus norvegicus) divided into 4 groups. The gingival injury was performed on mandible gingiva using a punch biopsy method with a 2mm diameter. The gel was given according to the treatment group 2 times a day for 7 days. Semendo coffee extract at 5%, 20%, and 40%, was applied to the wound treatment groups, while placebo gel was applied to the control group. Euthanasia was delivered on the 8th day, then histological preparation was made. The amount of fibroblast was analyzed by Olympus software. Result: The results showed that the Semendo coffee extract could significantly improve the number of fibroblast cells compared to the control groups. The highest fibroblast amount was found in the group with Semendo coffee extract at a concentration of 40%. Conclusion: Semendo coffee extract at 5%, 20%, and 40% increased the amount of fibroblast on the gingival wound in Rattus norvegicus.
ANTIBACTERIAL EFFECTIVENESS OF BEETROOT AGAINST STREPTOCOCCUS MUTANS Ulfa Yasmin; Ibnu Adjiedarmo; Yulia Christianti; Sulistiawati Sulistiawati; Mellani Cindera Negara
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 9, No 1 (2022)
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v9i1.938

Abstract

 Introduction: Antibacterial effectiveness is the level of an antibacterial agent’s ability in controlling bacteria through growth-inhibiting or killing activities. A natural antibacterial agent that has the potential to be used as an alternative is beetroot (Beta vulgaris L.) because it contains phenolic compounds, flavonoids, saponins, tannins, and betalains. Antibacterial agents have been widely used as preventive for dental caries in children with the main causative bacteria being Streptococcus mutans. This study aims to evaluate the antibacterial effectiveness of beetroot extract against Streptococcus mutans. Methods: This study was in vitro experimental laboratory study with a post-test-only control group design. The maceration method used to obtain beetroot extract which will diluted into various concentrations, 12,500 μg/ml, 25,000 μg/ml, 50,000 μg/ml, and 100,000 μg/ml. Antibacterial effectiveness was tested by measuring the inhibition zone using disc diffusion, determining the Minimum Inhibitory Concentration (MIC) using broth dilution, and the Minimum Bactericidal Concentration (MBC) using agar dilution with 0,2% chlorhexidine gluconate as the positive control. Analysis of inhibition zone data was performed statistically using one-way ANOVA and Tukey Post Hoc. MIC and MBC tests were analyzed descriptively. Results: The results show all concentrations of beetroot extract were able to form an inhibition zone with the largest mean was 0.40 ± 0.05 mm, but still lower than the positive control. MIC and MBC of extract were 25,000 μg/ml and 50,000 μg/ml respectively. Conclusion: It can be concluded that beetroot extract has antibacterial effectiveness against Streptococcus mutans, therefore it can be used as a natural alternative for dental caries prevention.
KARAKTERISASI HIDROKSIAPATIT YANG DISINTESIS DARI SISIK IKAN GABUS (Channa striata) DENGAN VARIASI SUHU KALSINASI Sulistiawati Sulistiawati; Siti Rusdiana; Dewi Shinta
Jurnal Kesehatan Gigi dan Mulut (JKGM) Vol 4 No 1 (2022): Jurnal Kesehatan Gigi dan Mulut (JKGM)
Publisher : Jurusan Keperawatan Gigi, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jkgm.v4i1.1181

Abstract

Latar Belakang: Hidroksiapatit merupakan salah satu material biokeramik dengan rumus kimia Ca10(PO4)6(OH)2 yang termasuk dalam senyawa kelompok mineral apatit yang terdiri dari kalsium dan fosfor. Sintesis hidroksiapatit dapat menggunakan bahan alami yaitu sisik ikan gabus (Channa striata). Tujuan: Tujuan penelitian ini untuk mengetahui perbedaan karakteristik hidroksiapatit dari sisik ikan gabus dengan variasi suhu kalsinasi. Metode: Penelitian ini mensintesis sisik ikan gabus dengan menggunakan prekrusor kalsium hidroksida (Ca(OH)2) dan asam fosfat (H3PO4) serta penambahan larutan NaOH. Endapan hidroksiapatit sisik ikan gabus yang dihasilkan lalu dikalsinasi menggunakan berbagai variasi suhu yaitu 700⸰C, 800⸰C, dan 900⸰C. Karakterisasi struktur derajat kristalinitas hidroksiapatit ditentukan dengan menggunakan x-ray diffraction (XRD) Hasil: Analisa XRD menunjukkan derajat kristalinitas hidroksiapatit sisik ikan gabus pada suhu suhu 700⸰C dengan tingkat derajat kristalinitas 67.7%, 800⸰C dengan tingkat derajat kristalinitas 79.2%, dan pada suhu 900⸰C dengan tingkat derajat kristalinitas 58.5% Kesimpulan: Suhu 800⸰C merupakan suhu optimum dalam sintesis sisik ikan gabus dikarenakan memiliki derajat kristalinitas yang paling tinggi. Kata Kunci: Sisik ikan gabus, derajat kristalinitas, hidroksiapatit, suhu kalsinasi, x-ray diffraction
PENGARUH PENAMBAHAN HIDROKSIAPATIT DARI CANGKANG TELUR TERHADAP KEKUATAN TEKAN GLASS IONOMER CEMENT Martha Mozartha; Muthiara Praziandithe; Sulistiawati Sulistiawati
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 2, Nomor 1, Juni 2015
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.878 KB) | DOI: 10.33854/JBDjbd.42

Abstract

Glass Ionomer Cement (GIC) memiliki sifat-sifat yang menguntungkan sebagai material restorasi. Namun, penggunaannya terbatas karena GIC memiliki kekuatan tekan yang rendah. Berbagai penelitian dilakukan untuk meningkatkan kekuatan tekan GIC, salah satunya penambahan hidroksiapatit ke bubuk GIC. Hidroksiapatit dapat disintesis dari larutan kimia atau berbagai limbah alam, misalnya cangkang telur, melalui metode presipitasi. Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan hidroksiapatit dari cangkang telur terhadap kekuatan tekan GIC Fuji IX (GC Corporation). Pembuatan 32 silinder GIC berdiameter 4mm dan tinggi 6mm dibagi menjadi 2 kelompok, yaitu: kelompok GIC tanpa penambahan hidroksiapatit sebagai kelompok kontrol (n=16) dan kelompok GIC dengan penambahan 8% hidroksiapatit sebagai kelompok uji (n=16). Kekuatan tekan diukur dengan Universal Testing Machine. Data dianalisis menggunakan uji T tidak berpasangan. Hasil pengukuran rata-rata kekuatan tekan GIC kelompok kontrol adalah 104,33±1,36 MPa dan kelompok uji adalah 109,52±1,58 MPa. Hasil uji T pada data tersebut menunjukkan perbedaan signifikan antar kelompok (p