I Putu Agus Hendra Wibawa
UPT Balai Konservasi Tumbuhan Kebun Raya “Eka Karya” Bali LIPI

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STUDI KERAGAMAN JENIS, KANDUNGAN GIZI ESENSIAL DAN KALSIUM OKSALAT DIOSCOREA DI PULAU BALI DAN LOMBOK Wibawa, I Putu Agus Hendra; Kurniawan, Agung; Adjie, Bayu
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) Juli 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Dioscorea tuber has many benefits, for example as food or as traditional medicine. The high carbohydrate and protein contents make this plant suitable as a food. However, the presence of crystal oxalate, that causes irritation is an disadvantage aspect on the development of this species. Both of wild and cultivated species will assure the gene flow which is important in efforts to improve the tuber quality should be examine further.  This research aimed to observe the biodiversity of Dioscorea in Bali and Lombok, to recognize the utilization and to reveal their nutrients (carbohydrate, protein and fat) and also the contents of calcium oxalate. The results showed that there are five Dioscorea spesies, namely  Dioscorea alata L. (ubi) (11 cultivars), Dioscorea esculenta (Lour.) Burkill (ubi aung), Dioscorea hispida Dennst. (gadung), Dioscorea bulbifera L. and Dioscorea pentaphylla L.   The highest carbohydrate content (87,42% ) is found in D. alata (ubi ungu cultivar) from Lombok,  while the highest protein content (5,05%.) is found in D. alata (ubi dadap cultivar) from Singaraja, Bali.  Furthermore, the highest percentage of fat content (0,85%) is found in D. bulbifera from Karangasem, Bali, whereas the high quantity of glaucomannan (44,52%.) id found in D. pentaphylla from Tabanan, Bali, while D. esculenta from Jembarana, Bali has the  highest calcium oxalate (0,57%), and D. alata (ubi injin cultivar) from Karangasem, Bali has the least calcium oxalate (0,18%).
STUDI KERAGAMAN JENIS, KANDUNGAN GIZI ESENSIAL DAN KALSIUM OKSALAT DIOSCOREA DI PULAU BALI DAN LOMBOK Wibawa, I Putu Agus Hendra; Kurniawan, Agung; Adjie, Bayu
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) July 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dioscorea tuber has many benefits, for example as food or as traditional medicine. The high carbohydrate and protein contents make this plant suitable as a food. However, the presence of crystal oxalate, that causes irritation is an disadvantage aspect on the development of this species. Both of wild and cultivated species will assure the gene flow which is important in efforts to improve the tuber quality should be examine further.  This research aimed to observe the biodiversity of Dioscorea in Bali and Lombok, to recognize the utilization and to reveal their nutrients (carbohydrate, protein and fat) and also the contents of calcium oxalate. The results showed that there are five Dioscorea spesies, namely  Dioscorea alata L. (ubi) (11 cultivars), Dioscorea esculenta (Lour.) Burkill (ubi aung), Dioscorea hispida Dennst. (gadung), Dioscorea bulbifera L. and Dioscorea pentaphylla L.   The highest carbohydrate content (87,42% ) is found in D. alata (ubi ungu cultivar) from Lombok,  while the highest protein content (5,05%.) is found in D. alata (ubi dadap cultivar) from Singaraja, Bali.  Furthermore, the highest percentage of fat content (0,85%) is found in D. bulbifera from Karangasem, Bali, whereas the high quantity of glaucomannan (44,52%.) id found in D. pentaphylla from Tabanan, Bali, while D. esculenta from Jembarana, Bali has the  highest calcium oxalate (0,57%), and D. alata (ubi injin cultivar) from Karangasem, Bali has the least calcium oxalate (0,18%).
Plant Collections of Bali Botanic Garden Potentially Used As Organic Pesticides Wibawa, I Putu Agus Hendra; Priyadi, Arief
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Along with society awareness of the importance of healthy life, demand of agricultural products which are pesticide residues free has been increasing. Recently, efforts to control plant pests and diseases by farmers have been relied on synthetic pesticides. Utilization of this kinds of pesticides results in various diseases such as cancer, fetal defects, infertility, liver diseases, and so on.  One of the alternatives which can be chosen to minimize those negative effects is using organic pesticides. Its ingredients are organic compounds which easily degraded, do not pollute the environment and relative safe for humans and livestock because its residues are degradable. Indonesia is one of countries with biggest biodiversity in the world, so it has great opportunities to produce organic pesticides. Bali Botanic Garden (BBG) is a government institution which plays role to conserve various kinds of plants from their natural habitats, especially the threatened species. This activity was aimed at knowing how many plant collections in BBG which have potency as organic pesticides and their specific functions whether as fungicides, insecticides, bactericides, rodenticides, nematicides, molluscicides, or fish poisons. The results show that there were 23 plants which have potency as organic pesticides. From that group, 22 kinds act as insecticides, 7 as fungicides, 5 as nematicides, 3 as molluscicides, rodenticides, fish poisons (pissicides), and bactericides respectively.Keywords: organic pesticides, Bali Botanic Garden, plant collections Keywords: organic pesticides, Bali Botanic Garden, plant collections
Association of Spheranthus africanus (Asteraceae) with other Weeds In a Fallowed Rice Field Priyadi, Arief; Wibawa, I Putu Agus Hendra; Lugrayasa, I Nyoman
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Spheranthus africanus is a minor weed of rice, which can be utilized for various purposes. It can be used for phytoremediation of soil contaminated with Pb and also reported to have anti-bacterial activity to be used as natural pesticides. However, because of its minority status little is known about this species in its natural habitat. This study was aimed to understanding the relationship between S. africanus with other weed species in a fallowed paddy field in Tabanan District, Bali, Indonesia. Vegetation analysis by quarter method was done in the rice field, where the species had occurred, in 2011 and 2012. Determination of association type between S. africanus with other weed was conducted by using 2x2 contingency table to calculate χ2, association coefficient (AC) and Ochiai index (OI). The result shows that the Shannon diversity index in this same area for the two consecutive years were 2.06 and 2.48 with species richness of 22 and 27 respectively. Calculation of Summed Dominance Ratio (SDR) showed that the species composition was not evenly distributed, in other words some species dominated others. In terms of association, although S. africanus mainly has no association with most of the weed species in the field, it prominently has positive association with Lindernia ciliata, and Digitaria ciliaris, with no significant negative association. Keywords: diversity, species association, Spheranthus africanus
Taking inventory of Orchid Types in a Region of Tambora Mountain - Sumbawa Island Wibawa, I Putu Agus Hendra; Peneng, I Nyoman; Priyadi, Arief
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Indonesia is well known as one of the countries with high biodiversity in the world, including its orchids. It is expected that there are 6,000 kinds of orchids which grow wildly in forest regions around the world. Along with accelerated forest destructions in Indonesia, due to land conversions or natural disasters, the existence of orchids in their natural habitats is threatened. It is likely that some of these richness could be lost before they can be conserved and known of their functions. Regions of Tambora mountain in Sumbawa island have biodiversity that had not been revealed. Therefore, taking inventory of flora in that region is very crucial to be done as soon as possible. This activity was aimed at saving biodiversity especially rare and endemic orchids there, and also quantitatively knowing richness and frequency of orchids species which are found. From the research, it is known that there were 22 orchids species, from which only one is terrestrial and the rests are epiphytes. In terms of richness, there were some orchids which were common and also rare. Keywords: orchids, Tambora Mountain, conservation, exploration
The Leaf Extract of Dysoxylum parasiticum (Osbeck) Kosterm. Contains Anti-oxidant and α-Glucosidase Inhibitor Activities: Leaf Extract of Dysoxylum parasiticum (Osbeck) Kosterm. Wibawa, I Putu Agus Hendra; Hanafi , Muhammad; Butardo Jr , Vito M.; Mahon, Peter J.
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.19

Abstract

The medicinal plant Dysoxylum parasiticum (Osbeck) Kosterm. (Meliaceae) is na- tive to Indonesia, but its bioactive potential has been little explored. This study de- termined the phenolic content, antioxidant, and antidiabetic activities of D. parasiti- cum leaf extracts. Dried, powdered leaves were sequentially extracted with hexane, ethyl acetate and methanol. Total phenolic and flavonoid contents were quantified by colorimetric assays. The ethyl acetate extract had the highest phenolic content at 21.54 mg gallic acid equivalents/g dry weight. Antioxidant capacity was evaluated by DPPH radical scavenging assay, while antidiabetic activity was assessed as inhi- bition of α-glucosidase enzyme. The ethyl acetate extract showed potent antioxidant (IC50 30.72 μg/mL) and α-glucosidase inhibitory (IC50 16.32 μg/mL) activities compared to standard controls. Putative bioactive components were identified by LC-MS/MS, including stylosin, capillartemisin A, 19β-glucosyl-14-deoxy-11,12- didehydroandrographoside, daturametelin H, stigmastan-3,6-dione, 2α,3β,4α-trihy- droxy nortropane and arnebinone. The traditional use of D. parasiticum is validated by its antioxidant and antidiabetic potential, which is associated with its phenolic content.
ANTIFUNGAL ACTIVITY OF SIAM CITRUS (Citrus nobilis L.) ESSENTIAL OIL AGAINTS Lasiodiplodia theobromae THE PATHOGEN OF BLENDOK DISEASE Pranata, Kadek Dwipayana; Wirawan, I Gede Putu; Wibawa, I Putu Agus Hendra; Suada, I Ketut; Wijaya, I Nyoman; Phabiola, Trisna Agung
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p08

Abstract

Siam citrus (C. nobilis L.) is a type of citrus that is most widely cultivated in Bangli Regency. The main disease that attacks citrus plants is blendok caused by the fungus L. theobromae. Based on research siam citrus peel contains essensial oils that have potential as antifungals. The aim of this study was to study chemical content of essensial oil from siam citrus peel and its activity as an antifungal againts L. theobromae. Essensial oil was destilled using hydro steam distillition and analyzed by means of GC-MS. Antifungal activiy testing was carried out using the agar-well diffusion method with concentrations of 1%, 10%, 25%, 50%, 75%, and 100%. Dithane M45 6 g/l and DMSO 10% were used as positive and negative controls. The results showed that the siam citrus peel from Kintamani, Bangli contains essensial oils with the main chemical components, i.e. D-limonene (57.26%), Beta-pinene (9.09%), and Beta-myrcene (4.03%) which has uses as an antifungal, additive, antitumor, asthma and allergy reliever, repellent, anti-inflammatory, antioxidant, anticancer, and antibacterial. Essential oil concentration of 25 to 100% can inhibit the growth of L. theobromae. The largest diameter of inhibition is shown at a concentration of 100% and the smallest diameter of inhibition is shown at a concentration of 25%. Keywords: C. nobilis L., essensial oils, GC-MS, L. Theobromae, antifungal
The Leaf Extract of Dysoxylum parasiticum (Osbeck) Kosterm. Contains Anti-oxidant and α-Glucosidase Inhibitor Activities: Leaf Extract of Dysoxylum parasiticum (Osbeck) Kosterm. Wibawa, I Putu Agus Hendra; Hanafi , Muhammad; Butardo Jr , Vito M.; Mahon, Peter J.
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.19

Abstract

The medicinal plant Dysoxylum parasiticum (Osbeck) Kosterm. (Meliaceae) is na- tive to Indonesia, but its bioactive potential has been little explored. This study de- termined the phenolic content, antioxidant, and antidiabetic activities of D. parasiti- cum leaf extracts. Dried, powdered leaves were sequentially extracted with hexane, ethyl acetate and methanol. Total phenolic and flavonoid contents were quantified by colorimetric assays. The ethyl acetate extract had the highest phenolic content at 21.54 mg gallic acid equivalents/g dry weight. Antioxidant capacity was evaluated by DPPH radical scavenging assay, while antidiabetic activity was assessed as inhi- bition of α-glucosidase enzyme. The ethyl acetate extract showed potent antioxidant (IC50 30.72 μg/mL) and α-glucosidase inhibitory (IC50 16.32 μg/mL) activities compared to standard controls. Putative bioactive components were identified by LC-MS/MS, including stylosin, capillartemisin A, 19β-glucosyl-14-deoxy-11,12- didehydroandrographoside, daturametelin H, stigmastan-3,6-dione, 2α,3β,4α-trihy- droxy nortropane and arnebinone. The traditional use of D. parasiticum is validated by its antioxidant and antidiabetic potential, which is associated with its phenolic content.