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All Journal International Journal of Biosciences and Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
TRISNA AGUNG PHABIOLA
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Phytochemical Content Identification And Antioxidant Activity Test Of Ethanol Extract Parijata Fruit (Medinilla Speciosa Blume) In Bedugul Area, Bali, Indonesia Mintari, Gusti Ayu Willem; Suada, I Ketut; Phabiola, Trisna Agung; Wijaya, Nyoman; Yuliadhi, Ketut Ayu; Bakar, Nor Kartini Abu
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p04

Abstract

Antioxidants are compound that function as free radical scavengers, these compounds can be found in parijata fruit (Medinilla speciosa Blume). The secondary metabolites of this fruit can be identified by the GC-MS method. The aims of study to identification of phytochemical content and test the antioxidant activity of the ethanol extract of Parijata fruit (Medinilla speciosa Blume) in the Bedugul area of Bali. This method is used because it is more sensitive and the results of the analysis are easy to understand. Using the GC-MS method, 11 organic compounds with a quality value of ? 90 were found in the ethanol extract of parijata fruit which have antioxidant, antimicrobial, and others. The 3 compounds with the highest Area Under Curve were 5-Hydroxymethylfurfural (AUC 31.05%); 1,2,3- Benzenetriol (AUC 22.32%); and 4H-pyran-4-one-2,3-dihydro-3,5-dihydroxy-6-methyl- (AUC 6.97%). These three compounds have antioxidant properties. Parijata fruit antioxidant activity test was tested with the DPPH method. The antioxidant activity of the ethanol extract of parijata fruit has an IC50 value of 11.26 mg/L, in the category of very strong antioxidant activity. So that with this category, parijata fruit can be given further treatment as food for health. In addition, compounds with antimicrobial properties can be used as biopesticide products in agriculture.
Identification and Antioxidant Activity Test of Strawberry Ethanol Extract Rosalinda and Osogrande (Fragaria x Ananassa) Varieties Enjelina, Ni Desak Gede Rista; Wijaya, I Nyoman; Suada, I Ketut; Phabiola, Trisna Agung; Utama, I Wayan Eka Karya; Yudha, I Kadek Wisma; Zhao, Tiejun
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p06

Abstract

Strawberries (Fragaria sp.) have many varieties spread across Indonesia. Pancasari Village, Buleleng is a strawberry producing area in Bali, there are several strawberry varieties cultivated by strawberry farmers in Bali, including the Rosalinda variety which has a sweet taste and the Osogrande has a sour taste. The purpose of this research is to determine the compounds contained in the fruits of these two varieties and to determine their antioxidant activity. Phytochemical analysis was performed using the GC-MS (Gas Chromatography-Mass Spectrometry) method, antioxidant activity was performed using the DPPH method. The results showed that the thick ethanol extract of (Fragaria x Ananassa) the Rosalinda and Osogrande varieties contained the same compound, namely 5-Hydroxymethylfurfural which has a function as an antioxidant, with 7 compounds that have known uses in the Rosalinda variety and 11 compounds in the Osogrande variety. The antioxidant activity of thick ethanol extract of the Rosalinda strawberry fruit is higher with IC50 of 10,05 ppm than the thick ethanol extract of Osogrande variety strawberry fruit with IC50 of 10,47 ppm, which means both are classified as having very strong antioxidant activity.
Phytochemical Screening and Acute Toxicity Test of Caulerpa lentillifera J. Agardh Infusion Extract on Mice (Mus musculus L.) Adiyanti, Sekar; Phabiola, Trisna Agung; Sutari, Ni Wayan Sri
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p03

Abstract

Caulerpa lentillifera has ingredients that are beneficial for health, such as anti-cancer, antioxidant, anti-diabetic, and immunostimulatory effects. This study was aimed to determine the content of phytochemical compounds and the LD50 acute toxicity dose of C. lentillifera infusion extracts on mice for their utilization as food or medicine. The phytochemical screening was done with a qualitative method. Twenty-five mice have been used for the acute toxicity test and divided into five groups of treatment (Control; 375 mg/kg BW; 750 mg/kg BW; 1.500 mg/kg BW; 3.000 mg/kg BW C. lentillifera infusion extracts orally). Symptoms of toxicity and mortality were observed 24 hours after treatment, and body weight was observed for seven days. The phytochemical screening result showed that the C. lentillifera infusion extracts contained alkaloids, saponins, and tannins. Acute toxicity tests showed no mortality in all treatment groups. Thus, the LD50 value could not be obtained and was classified as non-toxic.
ANTIFUNGAL ACTIVITY OF SIAM CITRUS (Citrus nobilis L.) ESSENTIAL OIL AGAINTS Lasiodiplodia theobromae THE PATHOGEN OF BLENDOK DISEASE Pranata, Kadek Dwipayana; Wirawan, I Gede Putu; Wibawa, I Putu Agus Hendra; Suada, I Ketut; Wijaya, I Nyoman; Phabiola, Trisna Agung
International Journal of Biosciences and Biotechnology Vol 11 No 2 (2024): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2024.v11.i02.p08

Abstract

Siam citrus (C. nobilis L.) is a type of citrus that is most widely cultivated in Bangli Regency. The main disease that attacks citrus plants is blendok caused by the fungus L. theobromae. Based on research siam citrus peel contains essensial oils that have potential as antifungals. The aim of this study was to study chemical content of essensial oil from siam citrus peel and its activity as an antifungal againts L. theobromae. Essensial oil was destilled using hydro steam distillition and analyzed by means of GC-MS. Antifungal activiy testing was carried out using the agar-well diffusion method with concentrations of 1%, 10%, 25%, 50%, 75%, and 100%. Dithane M45 6 g/l and DMSO 10% were used as positive and negative controls. The results showed that the siam citrus peel from Kintamani, Bangli contains essensial oils with the main chemical components, i.e. D-limonene (57.26%), Beta-pinene (9.09%), and Beta-myrcene (4.03%) which has uses as an antifungal, additive, antitumor, asthma and allergy reliever, repellent, anti-inflammatory, antioxidant, anticancer, and antibacterial. Essential oil concentration of 25 to 100% can inhibit the growth of L. theobromae. The largest diameter of inhibition is shown at a concentration of 100% and the smallest diameter of inhibition is shown at a concentration of 25%. Keywords: C. nobilis L., essensial oils, GC-MS, L. Theobromae, antifungal
Symptoms variation and molecular characterization of Strawberry vein banding virus in Bali, Indonesia Phabiola, Trisna Agung; Selangga, Dewa Gede Wiryangga; Wirya, Gusti Ngurah Alit Susanta; Sudiarta, I Putu; Dinarkaya, Shah Mahapati; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12544-53

Abstract

Vein banding disease has affected young strawberry leaves in Bali over the past five years. Caused by Strawberry vein banding virus (SVBV), the disease is primarily associated with fruit size reduction, though its exact impact remains unclear. This study aims to assess symptom variation, disease severity, and the molecular characteristics of SVBV in Bali strawberry plants. Field observations and molecular identification were conducted using PCR with SVBV-specific primers targeting the CP gene. Samples were collected from ten locations in Bali, including Pancasari, Candikuning, Wanagiri, Gobleg, and Kembang Merta. SVBV DNA from Candikuning, Pancasari, and Kembang Merta was successfully amplified, confirming SVBV infection as the cause of vein banding symptoms. SVBV induces vein banding with upward and downward leaf curling. Disease incidence was highest in Pancasari 1 and 3 (80%) and lowest in Wanagiri 1 and Gobleg (20–28%). Disease severity ranged from 13% to 83%, with the highest recorded in Pancasari, Buleleng, and the lowest in Kembang Merta, Tabanan. Molecular analysis revealed that the SVBV isolate from Bali shares 99.3–100% sequence homology with Chilean isolates. Phylogenetic analysis showed clustering with SVBV isolates from the United States, Brazil, and Chile. This study provides the first molecular characterization of SVBV in Bali, contributing to a better understanding of its epidemiology and potential impact on strawberry production.
Co-Authors Adiyanti, Sekar Bakar, Nor Kartini Abu Dinarkaya, Shah Mahapati Enjelina, Ni Desak Gede Rista Gusti Ngurah Alit Susanta Wirya I GEDE PUTU WIRAWAN I Ketut Suada I NYOMAN WIJAYA I Putu Agus Hendra Wibawa I Putu Sudiarta KETUT AYU YULIADHI Listihani, Listihani Mintari, Gusti Ayu Willem Ni Wayan Sri Sutari Pranata, Kadek Dwipayana Selangga, Dewa Gede Wiryangga UTAMA, I WAYAN EKA KARYA YUDHA, I KADEK WISMA Zhao, Tiejun