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All Journal AGRICOLA Musamus Journal of Livestock Science
Apri Irianto
Jurusan Peternakan Fakultas Pertanian Universitas Musamus
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Longivitas post ekuilibrasi spermatozoa sapi crossbreed Ongole menggunakan bahan pengencer yang disuplementasi Ekstrak buah merah Nurcholis Nurcholis; S M Salamony; Apri Irianto
AGRICOLA Vol 11 No 2 (2021): AGRICOLA
Publisher : Universitas Musamus, Merauke, Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/ag.v11i2.3228

Abstract

The use of Papuan red fruit as a supplementation ingredient in diluents is one solution to the high price of diluent raw materials. The purpose of this study was to determine the effect of using a diluent supplemented with red fruit extract (RFE) in various levels on the quality of liquid semen of Ongle crossbred cattle during storage at equilibration temperature. The research was conducted in the Animal Health Laboratory of Semangga District and the Independent Laboratory. The research method and design used were a completely randomized design (CRD) with 4 treatments and 5 replications. This study used 4 diluting treatments, namely P0 = Tris egg yolk (TKT), P1 = (TKT + 0.1 ml RFE), P2 = (TKT + 0.3 ml RFE) and P3 = (TKT + 0.5 ml RFE). Semen was collected using an artificial vagina, immediately after the semen storage was evaluated macroscopically and microscopically. The homogenized semen was divided into 4 tubes, then put the semen into the straw as much as 0.25 ml. The next process is equilibration at 5oC for 2, 4, 6, 8 hours and evaluation of spermatozoa longivity is carried out at pots equilibration. The results showed that there was a difference (P <0.05) in the longivity of spermatozoa in P3 treatment against the control. In conclusion, it can be concluded that the red fruit extract supplementation in egg yolk tris diluent was able to maintain spermatozoa longevity.
Keragaman Gen Myxovirus (Mx) pada Itik di Kabupaten Indragiri Hulu Provinsi Riau Restu Misrianti; Elfawati Elfawati; Apri Irianto
Musamus Journal of Livestock Science Vol 5 No 1 (2022): Musamus Journal of Livestock Science
Publisher : Faculty of Agriculture, Musamus University

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Abstract

The Mx gene (Myxovirus) is a gene that functions to control the immunity of poultry against viral diseases. This gene is a native antiviral and is able to respond to influenza virus infections such as avian influenza. This study aims to identify the diversity of the Mx gene in ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) molecular technique. The number of samples used were thirteen ducks from Indragiri Hulu Regency (Riau Province). The restriction site of the Mx gene using the Rsa1 enzyme is in the base sequence GTN|NAC. There are two alleles namely A and G, and three genotypes, AA, AG and GG. The results showed that the Mx gene in ducks was monomorphic. The frequency of the resistant allele (A) is 0%, while the frequency of the susceptible allele (G) is 100%.
Evaluasi Program Pemuliaan Sapi Bali Jantan di Sekolah Peternakan Rakyat Maju Bersama Kabupaten Musi Banyuasin Apri Irianto; Nurcholis Nurcholis
Musamus Journal of Livestock Science Vol 5 No 1 (2022): Musamus Journal of Livestock Science
Publisher : Faculty of Agriculture, Musamus University

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Abstract

Musi Banyuasin is one of the regency in South Sumatra Province. The population of cattle based on 2017 data is 31,834 heads spread across 14 districts. The type of cattle that is mostly kept is Bali cattle which are scattered throughout the Musi Banyuasin. SPR Maju Bersama is one of the SPRs under the guidance of IPB University in Musi Banyuasin. The purpose of this study was to evaluate the male Bali cattle breeding program at SPR Maju Bersama. The method used in this study is purposive sampling with the observed parameters, namely the pattern of maintenance, the mating system of male sires and the relationship between the rearing system and the results of the livestock BCS assessment as a program evaluation parameter. The results of this study indicate that SPR Maju Bersama is quite good in carrying out the male Bali cattle breeding program as superior breeds. The BCS value is at a score of 3 with a total of 53 heads from the total sample population of 89 heads.
Co-Authors Elfawati Elfawati Nurcholis Nurcholis Nurcholis Nurcholis Restu Misrianti S M Salamony