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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture
R. Widiastuti
Toxicology Department, Indonesian Research Center for Veterinary Science (IRCVS) Jl. R.E. Martadinata 30, Bogor 16114
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Detection of deoxynivalenol and T-2 toxin in feeds by gas chromatography with Electron Captured Detector Widiastuti, R.; ., Indraningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.431 KB) | DOI: 10.14334/jitv.v14i3.345

Abstract

Deoxynivalenol (DON) and T-2 toxin are trichothecene mycotoxins produced by Fusarium spp. may pose to threat animal health. This research was aimed to validate an analysis method for DON and T-2 toxin detection in feed using a gas chromatography with electron captured detector ECD (GC-ECD) and to study the contamination level of DON and T-2 toxin in feed. Samples were extracted with organic solvents and derivitized with DMAP and HFBA prior to the detection with GC. Both toxins can be detected simultaneously with satisfied validation with GC. Analysis for DON showed that 17 out of 24 corn samples were positive (0.450 to 1.126 mg/kg), none was positive for 20 rice husk samples and 1 out of 14 cattle feed samples was positive (0.043 mg/kg), whereas analysis for T-2 toxin showed that 2 out of 24 corn samples were positive (0.022 and 0.063 mg/kg), 14 out of 20 rice husk samples were positive (0.027 to 0.720 mg/kg) and 6 out of 14 cattle feed samples were positive (0.039 to 0.084 mg/kg). The study showed that DON and T-2 toxin can be detected using GC-ECD. Whereas, the analysis results from field samples showed that the feeds were still safe to be used for animal feed, however a routine surveillance is needed to minimize further potential hazards. Key words:  Deoxynivalenol, T-2 toxin, Gas Chromatography, Feeds
DETECTION OF OXYTETRACYCLINE IN BROILER CHICKEN MEAT MARKETED IN SEVERAL CITIES IN JAVA ISLAND USING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) METHOD Widiastuti, R.; Anastasia, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.1.52-58

Abstract

Oxytetracycline (OTC) is one of the tetracycline (TCs) broad-spectrum antibiotics widely used inthe chicken industry. However, improper use of OTC with excessive doses potentially leads to residueformation in animal products that can be harmful to consumers in the form of allergic reaction orresistance. This study aimed to detect OTC residues in broiler chicken meat, marketed in traditionalmarkets and supermarkets in Depok, Bekasi, Bandung, Cilegon, Surakarta and Yogyakarta using indirectcompetitive enzyme-linked immunosorbent assay (icELISA) method. The analyses of 67 broiler meatsamples showed only 1 (1.5%) sample was positive for the OTC residue at 86.1 ng/g which meant belowthe maximum residue limits of permissible OTC (100 ng/g). Nevertheless, a stricter regulation for theuse of OTC in the poultry industry and the monitoring of its residue in chicken products prior tomarketing is still necessary to avoid the adverse effects of the residue present in animal products.
Co-Authors Indraningsih . Y. Anastasia, Y.