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All Journal Ganendra: Majalah IPTEK Nuklir Indonesian Journal of Cancer Chemoprevention INDONESIAN JOURNAL OF PHARMACY
Eva Maria Widyasari
Nuclear Material and Radiometric Technology Center – BATAN, Bandung
Biochemistry, Genetics & Molecular Biology Computer Science & IT Medicine & Pharmacology Other

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Labelling of human serum albumin (HSA)-nanospheres with technetium-99m radionuclide Oekar, Nanny Kartini; Widyasari, Eva Maria
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 3, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.018 KB) | DOI: 10.14499/indonesianjpharm0iss0pp117-127

Abstract

Labelling of HSA-nanospheres with technetium-99m was carried out by direct and indirect method using sodium pyrophosphate as co-ligand agent. The labelling efficiency was determined by several chromatography system for separation of 99mTc-HSA-nanospheres labelled compound from its radiochemical impurities. Several parameters influencing the labelling process were studied such as pH, kinds and quantity of reductor agent, labelling method, quantity of HSA-nanospheres and temperature and the duration of incubation. The result show that the optimum direct labelling condition was found by using 0.5 mL HSA-nanospheres solution (which had absorbance of 0.6 at l= 202 nm), 100-150 μg of SnCl2.2H2O as reductor, pH mixture was 2 and the first incubation was done at room temperature for 25 minutes. The labelling process was continued by adding technetium-99m of certain activity, and finally pH was adjusted to 5.5-6.0. The second incubation was carried out at room temperature for 15 minutes. This direct method resulted more than 90% of labelling efficiency, with free 99mTc-pertechnetate as radiochemical impurity. The indirect labelling process by using 0.5 mL of HSA-nanospheres solution, 100 μg of SnCl2.2H2O was prior reacted with 1.0 mg of sodium pyrophosphate (1 : 5 mol/mol), the pH was adjusted to 7.4 and incubation was done in the incubator at 37oC for 15 minutes. After adding 99mTc-pertechnetate solution, the second incubation was done at room temperature for 15 minutes. This indirect labelling resulted 93.4 ± 1.2 % of labelling efficiency with remain of 99mTc-pertechnetat and 99mTc-pyrophosphate as radiochemical impurities.Key words: lymphoscintigraphy, nanocolloid, technetium-99m, radiochemical impurities.
STUDI AWAL BIODISTRIBUSI NANOMATERIAL 186Re-M41S-NH2 SEBAGAI RADIOFARMAKA UNTUK PROSEDUR RADIOSINOVEKTOMI Sugiharti, Rizky Juwita; Daruwati, Isti; Widyasari, Eva Maria; Christina, Maria
GANENDRA Majalah IPTEK Nuklir Volume 22 Nomor 1 Januari 2019
Publisher : Website

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.065 KB) | DOI: 10.17146/gnd.2019.22.1.4724

Abstract

Rheumatoid arthritis merupakan penyakit autoimun akibat reaksi antigen-antibodi pada lapisan membran sinovial yang menyebabkan kerusakan pada sendi. Salah satu prosedur radioterapi yang digunakan untuk meredakan nyeri dan inflamasi yang disebabkan oleh rheumatoid arthritis adalah radiosinovektomi yaitu dengan injeksi radioisotop pemancar sinar β secara intra-artikular ke dalam sendi sinovial. M41S-NH2adalah partikel ordered mesoporous silica dengan pori-pori seragam yang berikatan stabil dengan radioisotop renium-186 (186ReO4-) menjadi 186Re-M41S-NH2 yang dikembangkan sebagai radiofarmaka untuk prosedur radiosinovektomi. Untuk mengetahui pola biodistribusi dan stabilitas radiofarmaka 186Re-M41S-NH2 secara in vivo maka dilakukan uji biodistribusi dengan cara menyuntikan 186Re-M41S-NH2secara intra artikular ke dalam sendi tikus normal stok Sprague Dawley. Hasil uji biodistribusi 186Re-M41S-NH2, memperlihatkan partikel radiofarmaka 186Re-M41S-NH2 terakumulasi sebesar (96,80+3,92)% ID di dalam sendi lutut 3 jam pasca injeksi.  Eksresi  radiofarmaka  186Re-M41S-NH2di dalam urin setelah 24 jam adalah sebesar (6,11+0,5)% ID menunjukkan radiofarmaka ini cukup stabil di dalam rongga sendi. Data biodistribusi radiofarmaka 186Re-M41S-NH2memperlihatkan radioaktivitas yang sangat rendah di semua organ non target khususnya di hati dan limpa. Akumulasi radioaktivitas yang kecil di lambung menunjukkan radiofarmaka 186Re-M41S-NH2merupakan kompleks yang stabil secara in vivo dan tidak terurai menjadi ReO4-. Dari uji pendahuluan biodistribusi ini memperlihatkan bahwa radiofarmaka 186Re-M41S-NH2ini cukup ideal sebagai radiofarmaka yang dapat digunakan untuk prosedur radiosinovektomi. 
The Optimization Method for Synthesis of 99mTc-Rutin as Potential Radiotracer in The Development of Cancer Drugs From Flavonoid Widyasari, Eva Maria; Kusumawardhany, Esty; Sugiharti, Rizky Juwita; Sriyani, Maula Eka; Marzuki, Muharam
Indonesian Journal of Cancer Chemoprevention Vol 10, No 2 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss2pp80-87

Abstract

Based on the Basic Health Research Data of Ministry of Health's of Indonesia in 2013, mortality rates from malignant and tumor malignancies in Indonesia are still high with prevalence of cancer is about 1.4%. Chemotherapy is still the primary choice in cancer modality that uses chemotherapeutic drugs to eradicate and inhibit the growth of cancer cells; however the cost this treatment is extremely high. Therefore, patient tends to seek alternative treatment such as consuming traditional herbal medicine for cancer treatment. Rutin is one of the attractive phytochemicals flavonoids because of its antioxidant activities. However, as traditional herbal medicine, its effectiveness is not yet been fully established due to the lack of scientific information. A radiotracer can be defined as a specific radiolabeled molecule that monitors the in vivo behaviour of a functional molecule, and can be used to provide biological information in a living system. Hence, to provide pharmacological information of rutin for cancer treatment, we synthesized radiolabeled flavonoid 99mTc-rutin as radiotracer. The aim of the present study is to develop 99mTc-rutin under varying conditions of rutin quantity, reducing agent concentration and incubation time. Labeling studies were performed by changing the selected parameters one by one and optimum labeling conditions were determined. After observing the conditions for maximum labeling efficiency, 99mTc-rutin was obtained with preparation of 700 μg of rutin with addition of 20 μg of SnCl2.2H2O as reductor and 1-3 mCi 99mTcO4- without any incubation. Radiochemical yield of 99mTc-rutin was determined with radio thin layer chromatography which was found 99.28 ± 0.14% and stable up to 4 hour. From the result of this study, the successfully labeled 99mTc-rutin can be used as a reference for following preclinical study. Furthermore radiolabeled 99mTc-rutin is expected as tools in research and development of rutin as cancer drugs from natural product to obtain detailed information its efficacy.Keywords: radiotracer, 99mTc-rutin, cancer, labeled compounds
Co-Authors Christina, Maria Isti Daruwati Kusumawardhany, Esty Marzuki, Muharam Maula Eka Sriyani Nanny Kartini Oekar Rizky Juwita Sugiharti