Kristiana Sri Wijayanti
Jln. Raya Karangploso km 4, Kotak Pos 199, Malang 65152, Indonesia

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Keefektifan Kalsium Polisulfida terhadap Rhizoctonia solani dan Rhizoctonia bataticola secara In Vitro Hidayah, Nurul; Wijayanti, Kristiana Sri; Asbani, Nur
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 4, No 1 (2012): April 2012
Publisher : Balai Penelitian Tanaman Pemanis dan Serat (Balittas)

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Abstract

Rhizoctonia solani dan R. bataticola merupakan jamur patogen pada tanaman kapas yang sulit dikendalikan, karena dapat menghasilkan sklerosia sebagai struktur istirahatnya di dalam tanah meskipun tidak ada inang. Perlindungan tanaman sejak awal perlu dilakukan untuk melindungi dari serangan patogen tersebut. Pengguna-an kalsium polisulfida yang merupakan pestisida ramah lingkungan dapat menjadi alternatif untuk mengenda-likan penyakit yang diakibatkan kedua jamur tersebut. Penelitian bertujuan untuk mengidentifikasi kemampuan kalsium polisulfida dalam menghambat pertumbuhan jamur R. solani dan R. bataticola secara in vitro. Tu-juh level konsentrasi kalsium polisulfida yakni 0% (kontrol); 0,5%; 1%; 1,5%; 2%; 2,5%; dan 3% masing-masing dituang ke dalam cawan petri kemudian ditambahkan dengan 10 ml media PDA (Potato Dextrose Agar). Inokulum R. solani dan R. bataticola masing-masing secara terpisah diinokulasikan setelah media pa-dat dan diinkubasi pada suhu kamar. Hasil penelitian menunjukkan bahwa secara in vitro, kalsium polisulfida hanya mampu menghambat pertumbuhan miselia jamur R. solani dan R. bataticola sampai dengan hari kedua setelah perlakuan. Setelah itu persentase penghambatannya berangsur-angsur menurun. Both of Rhizoctonia solani and R. bataticola are the difficult fungal pathogens to control since they can pro-duce sclerotia as the resting spore in the soil even though there is no host. An early plant protection is im-portant to defend from the pathogen infection. The use of calcium polysulfide, an environmentally friendly pesticide, could be an alternative method to control diseases caused by both of them. The objective of this research was to identify the potency of calcium polysulfide in inhibiting of R. solani and R. bataticola growth in vitro. There were seven level of concentration of calcium polysulphide, ie. 0% (control), 0.5%, 1%, 1.5%, 2%, 2.5%, and 3%, were poured onto petridish and added by PDA medium. R. solani and R. bataticola inocula were inoculated onto agar plate separately and incubated in room temperature. The result indicated that calcium polysulfide could inhibit the growth of R. solani and R. bataticola in vitro until two days after inoculation (dai), after that its capability was decreased slowly.
Aktivitas Antibakteri Minyak Atsiri Daun Tembakau terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Nurnasari, Elda; Wijayanti, Kristiana Sri
Jurnal Kefarmasian Indonesia VOLUME 9, NOMOR 1, FEBRUARI 2019
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v9i1.1219

Abstract

Tobacco plants are widely used as raw material for cigarettes, but the results of the study show that in tobacco leaves contain compounds that can be used in the pharmaceutical and health fields. Tobacco leaves contain essential oils that have a distinctive aroma. The study aims to examine the antibacterial activity of tobacco essential oil from six sources tobacco such as Temanggung, Yogyakarta, Purwodadi, Boyolali, Blitar and Probolinggo. Tobacco essential oil is obtained by the steam-water distillation method. Antibacterial activity evaluation was carried out by diffusion method and the minimum inhibitory concentration on two bacteria, Escherichia coli and Staphylococcus aureus. The yield of tobacco essential oil ranged from 0.54-3.39%. Tobacco essential oil had antibacterial activity against bacteria E. coli and S. aureus in the presence of inhibition zones in bacterial media. The biggest inhibition zone was in Yogyakarta tobacco essential oil of 26 mm for S. aureus bacteria, while for E. coli bacteria the biggest inhibition zone was Blitar tobacco essential oil which was equal to 21 mm. The inhibition zone was not much different from the inhibition zone in tetracycline antibiotic testing (positive control) which was 25 mm in S. aureus and 21.5 mm bacteria in E. coli bacteria. Tobacco essential oil from Probolinggo was able to inhibit the growth of S. aureus bacteria up to a concentration of 6.25% and E. coli bacteria to a concentration of 12.5%.