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All Journal Jurnal Ilmu Ternak Veteriner Animal Production : Indonesian Journal of Animal Production
W Winarsih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Spontaneous cholangiohepatitis in broiler chickens: immunohistochemical study of Ito cells Handharyani, E; Ochiai, K; Winarsih, W; Harlina, E; Estuninngsih, Salfina Nurdin
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 4 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.803 KB) | DOI: 10.14334/jitv.v6i4.245

Abstract

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis andmalformation of extrahepatic biliary tracts.   Key words : Cholangiohepatitis, immunohistochemistry, ito cell, broiler chicken
Organic Acid Salt from Complete Feed Silage Corn Based by Product as an Alternative to Substitute Antibiotic Function as a Growth Promotor for Broiler Negara, W; Ridla, M; Lubis, AD; Winarsih, W; Surachman, M; Darmawan, IWA; Santoso, Santoso
ANIMAL PRODUCTION Vol 11, No 3 (2009): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.274 KB)

Abstract

This study was designed to evaluate the efficacy of organic acid salt Zn from complete feed silage based on corn by product as an alternative to subtitute antibiotic function as a growth promotor for broiler. Ninety day old commercial Cobb broiler chickens were randomly distributed into six groups having three replicates of five birds in each group. Negative control (R0) birds were offered standard basal diet and no challenged, positive control (R1) birds were offered standard basal diet and challenged with 107 Salmonella typhimurium. Treatment R2, R3, R4 and R5 were challenged by 107 CFU of Salmonella typhimurium which added in feed with 0.1% flouroquinolone, 0.1%, 0.2%, and 0.3% of organic acid salts. The result showed that dietary of organic acid salts affect consumption, weight gain, and final body weight (P<0.05). Meanwhile, feed conversion (FCR) was not affected by antibiotics nor  organic acids. Our conclusion, Dietary organic acid salt from complete feed silage corn based by product until dose 0.2% can improve the performance of broiler chickens infected Salmonella typhimurium. (Animal Production 11(3): 170-175 (2009) Key Words: broiler, organic acid, Salmonella typhimurium
Spontaneous cholangiohepatitis in broiler chickens: immunohistochemical study of Ito cells E Handharyani; K Ochiai; W Winarsih; E Harlina; Salfina Nurdin Estuninngsih
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.803 KB) | DOI: 10.14334/jitv.v6i4.245

Abstract

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis andmalformation of extrahepatic biliary tracts.   Key words : Cholangiohepatitis, immunohistochemistry, ito cell, broiler chicken
Co-Authors AD Lubis, AD E Handharyani E Harlina IWA Darmawan, IWA K Ochiai M Surachman Muhammad Ridla Salfina Nurdin Estuninngsih SANTOSO SANTOSO W Negara, W