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Extrahepatic bile duct ligation in broiler chickens: ultrastructural study of Ito cell Handharyani, Ekowati; Ochiai, K; Winarto, A
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 4 (2004)
Publisher : Indonesian Animal Sciences Society

The Ito cell (fat-storing cell) is a cell lying in perisinusoidal space of liver. The function of Ito cell is expanding from a site of fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. This study was performed in order to evaluate the Ito cells in cholestatic condition. The artificial cholestatic was conducted by ligation of extrahepatic bile ducts (bile duct ligation = BDL) in broilers. The results showed that BDL induced bile congestion, fibrosis, proliferation of Ito cells and intrahepatic bile ductules. Immunohistochemistry demonstrated that Ito cells were scattered throughout the fibrotic areas, and larger in size with more extensive immunoreactivity than those in normal livers. Ultrastructural study demonstrated that Ito cells were closely associated with the production of extracellular collagen fibers. Ito cells actively react against hepatocytic injuries, especially in fibrogenesis of cholestatic livers. Â Key words: Bile duct, ito cell, broiler

The Histological of Ewe Ovarium Post-Intrauterine Transplantation to Pseudopregnancy Rabbit Sumarmin, R; Boediono, A; Winarto, A; Yusuf, TL
ANIMAL PRODUCTION Vol 10, No 2 (2008): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objective of this study was to evaluate the histologycal of ewe ovarium post-intrauterin transplantation to pseudopregnancy rabbit. The experiment was concerned with the 1 or 7 days of pseudopregnancy to receive the ewe ovarian transplant. Post transplantation 5, 7 or 9 days of ewe ovarium were recollected. To determine histologically post-intrauterine transplantation of ewe ovarium, the histological preparat was prepared by the paraffin methods followed by HE staining. The result showed that there were follicle development with all stages of follicle dynamics (Primordial, Primary, Prentral and Antral follicle stages) in all group of treatment. The ewe ovarian post-intrauterine transplantation damaged was found as follicles degeneration, ephitellial damaged and the formation of protein aggregate. It can be concluded that the histological of ewe ovarium post-intrauterine transplantation in pseudopregnancy rabbit is still good in the 5 or 7 days after intrauterine transplantation in pseudopregnancy rabbit. (Animal Production 10(2): 78-84 (2008)Â Key Words: Histological,Â post-intrauterine transplantation, ewe ovarium

Improvement of Lamb Preweaning Performance by Combination of Superovulation of Ewes Prior to Mating and Temulawak Extract Plus Administration During Pregnancy Andriyanto, Andriyanto; Darulfallah, MD; Arif, R; Nugraha, GM; Winarto, A; Manalu, W
ANIMAL PRODUCTION Vol 14, No 3 (2012): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. Superovulation is one of a reproductive technology to improve livestock productivity. The research was conducted to optimize the superovulation technology by combining it with administration of temulawak extract plus during pregnancy. Sixteen ewes were injected prostaglandin hormone at a dose of 10 mg/kg body weight intramuscularly twice, with eleven days interval, to synchronize estrous cycle. On the eleventh day, superovulation wasÂ induced by injection of pregnant mare serum gonadotrophinÂ hormone at a dose of 200 IU/ewe intramuscularly. The ewes showing the estrous signs were mated naturally. Temulawak extract plus was administered weekly during pregnancy with a dose of 1 mg/kg body weight. Parameters measured in this study were lambs birth weight and preweaning growth. Superovulation increased average litter size. The result showed that, regardless of litter size, superovulation increased lambs birth weight by 15% as compared to controls and temulawak extract plus groups. Superovulation before mating and temulawak extract plus administration during pregnancy improved lambs growth in the first month and the third month postpartum. Superovulation prior to mating increased lambs birth weight and improved lambs growth performance before weaning.Â Keywords: superovulation, temulawak extract plus, lambs growth performance, ewesÂ Abstrak.Â Superovulasi merupakan salah satu teknologi reproduksi untuk meningkatkan produktivitas ternak. Penelitian ini dilakukan untuk mengoptimalkan teknologi superovulasi dengan menggabungkan pemberian ekstrak temulawak plus selama kebuntingan. Enam belas domba disuntik hormon prostaglandin dengan dosis 10 mg/kg bobot badan secara intramuskular sebanyak dua kali, dengan selang waktu sebelas hari, untuk menyesuaikan siklus estrus. Pada hari ke sebelas, superovulasi diinduksi dengan injeksi pregnant mare serum gonadotrophin hormon dengan dosis 200 IU/domba secara intramuskuler. Domba yang menunjukkan tanda-tanda estrus kemudian dikawinkan secara alami. Ekstrak temulawak plus diberikan setiap minggu selama kebuntingan dengan dosis 1 mg/kg bobot badan. Parameter yang diukur dalam penelitian ini adalah bobot lahir anak domba dan pertumbuhan prasapih. Superovulasi meningkatkan rata-rata litter size. Hasil penelitian menunjukkan bahwa, terlepas dari litter size, superovulasi meningkatkan bobot lahir anak domba sebesar 15% dibandingkan dengan kelompok ekstrak temulawak plus. Superovulasi sebelum kawin dan pemberian ekstrak temulawak plus selama kebuntinganÂ meningkatkan pertumbuhan anak domba di bulan pertama dan bulan ketiga paska melahirkan. Superovulasi sebelum perkawinan meningkatan berat lahir anak domba dan meningkatan performa pertumbuhan anak domba sebelum penyapihan.Â Kata kunci: superovulasi, temulawak ekstrak plus, kinerja pertumbuhan anak domba, dombaÂ Andriyanto et al/Animal Production 14(3):167-172, September 2012

Histomorphology and Physical Characteristics of Buffalo Meat at Different Sex and Age H Nuraini; . Mahmudah; A Winarto; C Sumantri
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this experiment was to evaluate histology and physical variables of buffalo meat at different sex and ages. Thirty head of buffaloes were used in this experiment. Muscle fiber diameter, fasciculus diameter, thickness of connective tissue, pH, water holding capacity, Warner-Bratzler shear force and cooking loss were measured in this experiment. Analysis of variance of randomized factorial design was used to analyse the data. The differences were analysed by Tukey test. The result showed that muscle fiber diameter was influenced by ages. The difference muscle fiber diameter among ages indicated that 8-12 mo and 1.5 yr old have smaller diameter compared to three year old (P<0.05), but there was no differences between two, three and four years old. The fasciculus diameter, thickness of connective tissue, tenderness, pH value, water holding capacity, and cooking lost indicated no significant found in all stage of age and sex of samples. Meat qualities of buffaloes were not affected by age (2-4 yr) and sex.

PAT-1 Case report: Metastatic ganglioneuroma in a Sumatran Tiger E Handharyani; D R Agungpriyono; R W Wiranti; G K Suryaman; S Edwar; E Rumiyati; A Winarto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Neuroblastic tumors, which are uncommon tumors of nerve cell origin, are classified into neuroblastoma, ganglioneuroblastoma, and ganglioneuroma depend-ing on the degree of maturation of neoplastic neuroblasts and development of schwannian stroma [2,4,7]. Ganglioneuroma is a rare neuroectodermal tumor of the peripheral nervous system. Ganglio-neuromas generally are considered to be benign neoplasms composed of both mature ganglion cells and nerve fascicles with axons, Schwann cells, fibroblasts, and other connective tissue elements [1]. The aim of this study is to clarify the pathomor-phological feature of a subcutaneous ganglio-neuroma which occurred in a Sumatran tiger.

Extrahepatic bile duct ligation in broiler chickens: ultrastructural study of Ito cell Ekowati Handharyani; K Ochiai; A Winarto
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The Ito cell (fat-storing cell) is a cell lying in perisinusoidal space of liver. The function of Ito cell is expanding from a site of fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. This study was performed in order to evaluate the Ito cells in cholestatic condition. The artificial cholestatic was conducted by ligation of extrahepatic bile ducts (bile duct ligation = BDL) in broilers. The results showed that BDL induced bile congestion, fibrosis, proliferation of Ito cells and intrahepatic bile ductules. Immunohistochemistry demonstrated that Ito cells were scattered throughout the fibrotic areas, and larger in size with more extensive immunoreactivity than those in normal livers. Ultrastructural study demonstrated that Ito cells were closely associated with the production of extracellular collagen fibers. Ito cells actively react against hepatocytic injuries, especially in fibrogenesis of cholestatic livers. Key words: Bile duct, ito cell, broiler

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