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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biotropika Journal of Fisheries & Marine
ekwan nofa wiratno
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Journal : Biotropika

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Pengaruh Gula Reduksi dan Total Nitrogen Terhadap Densitas dan Viabilitas Sel Saccharomyces cerevisiae dalam Fermentasi Etanol dari Molase wiratno, ekwan nofa; Ardyati, Tri
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.307 KB)

Abstract

Fermentasi etanol dipengaruhi oleh beberapa faktor, salah satu diantaranya adalah densitas dan viabilitas sel khamir. Penelitian ini bertujuan mengetahui pengaruh variasi total nitrogen dan gula reduksi terhadap densitas dan viabilitas sel Saccharomyces cerevisiae (SAF Instan) selama proses fermentasi etanol dari molase. Tahapan penelitian ini terdiri dari pengukuran kadar gula reduksi, total nitrogen, konsentrasi kalsium, penentuan densitas dan viabilitas sel khamir serta analisis data. Fermentasi dilakukan selama 72 jam dengan 3 ulangan. Perlakuan konsentrasi gula reduksi (GR) dan total nitrogen (N) yaitu GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 dan GR 125 N 10 g/l. Penghitungan viabilitas sel dilakukan setiap 24 jam. Viabilitas tertinggi adalah GR 125 N 0 (95,76 %) dan GR 100 N 6 (95,96 %). Penurunan viabilitas sel disebabkan oleh rendahnya perkembangbiakan sel khamir (tidak terjadi peningkatan jumlah sel secara signifikan (p>0,05)). Hal ini terjadi karena tingginya konsentrasi kalsium (0,21 %).
Isolation, Identification and Ethanol Production of Indigenous Yeast of Toddy Palm (Borassus Flabellifer L.) Juice From Tuban, East Java, Indonesia Wiratno, Ekwan Nofa; Rupilu, Novi Soleman
Biotropika: Journal of Tropical Biology Vol 6, No 1 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.075 KB) | DOI: 10.21776/ub.biotropika.2018.006.01.2

Abstract

This study aimed to obtain and identify the indigenous yeasts isolated from Borassus flabellifer L. juice and its potency in ethanol production. Steps of this study included isolation, enumeration, characterization, identification and ethanol production and measurement of ethanol concentrations and medium acidity during fermentation. The yeast total isolated from Borassus flabellifer L. juice was 5.06 x 105 CFU/mL and the isolate U2 was phenotypically identified as Candida tropicalis (95.7% ID and T = 1). The pH level of medium decreased during the ethanol fermentation. Yeast isolate U2 was able to produce ethanol by 140.1 g/L using 50 g/L of glucose and it was not significantly different with ethanol yield fermented by Saccharomyces cerevisiae (148.6 g/L).
Abundance and Phylogenetic Analysis of High-Density Polyethylene (HDPE) Biodegrading Bacteria from Brantas River, Malang City Wiratno, Ekwan Nofa; Sandra Wardani, Erika; Agustin, Nurmalita
Biotropika: Journal of Tropical Biology Vol. 12 No. 2 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.02.06

Abstract

Microplastic pollution has become a global concern after the COVID-19 pandemic. This requires efforts to resolve the various adverse effects of microplastic pollution in the aquatic environment. This study aimed to isolate, enumerate, and determine the potency of microplastic biodegrading bacteria from the Brantas River, Malang City. This research includes environmental factor measures, bacterial isolation, enumeration, biodegradation would be produced (qualitative and quantitative), DNA isolation, PCR, sequencing, phylogenetic analysis, and data analysis. The environmental conditions of the sampling locations are generally homogeneous. The river temperature observed ranged from 24.1 to 27.2 °C. Research locations that have acidity ranging from 7.35 to 8.16. The salinity of the Brantas River ranges from 205-306 ppm or 0.0205-0.306%. The conductivity of the Brantas River ranges from 410-612 µS/cm. Isolation produced six different bacterial isolates. The amount of each isolate varies in each sample. A qualitative microplastic biodegradation test was indicated by the growth of colonies in the plastic area. This result shows that the bacterial isolate uses plastic as a carbon source. All isolates grow around plastic. Quantitative tests using HDPE sheets show that not all isolates can biodegrade microplastics. Bacterial isolates capable of biodegrading were isolates K2 (5.41%), K22 (5.16%), and K5 (6.69%). Based on phylogenetic analysis, it is known that isolate K2 has a similarity of 36.4% to ON845428.1 Klebsiella sp. strain 214 37 z 3, and ON845427.1 Klebsiella sp. strain 213 37 z 1. Isolate K2 has a distance score of 1.268 to MZ642649.1 Klebsiella variicola strain PDW768. Isolate K22 has a similarity of 36.8% with KT895299.1 Enterobacter cloacae strain Jilu WG154. The distance score of isolate K22 showed values of 1.179 and 1.156 against MH796357.1 Enterobacter sp. strain 3C, and OQ813771.1 Enterobacter cloacae strain TBMAX59. Similarity and distance score values consistently showed that isolates K2 and K22 were not identified at the genus level. These two isolates have the potential to be recognized as new species.
Co-Authors AGUSTIN KRISNA WARDANI Agustin, Nurmalita Amin, Abd. Aziz Andi Kurniawan Dewi, Citra Satrya Utama Hanafi, Nafizatun Eliana Ali Huyop, Fahrul Zaman Jamhari Jamhari Redzuan, Rabiatul Alia Rozdhl, Amira Azawani Mohd Rupilu, Novi Soleman Salamah , Lutfi Ni'matus Sandra Wardani, Erika Sujadi, Frentina Murty Tri Ardyati Yanuar , Adi Tiya