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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Biospecies BIOTROPIA - The Southeast Asian Journal of Tropical Biology Buletin Kebun Raya BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Reinwardtia Jurnal Integritas Serasan Sekundang
Joko Ridho Witono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Social Sciences Veterinary

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Journal : BERITA BIOLOGI

 1 
KILAS BALIK PENELITIAN KROMOSOM PALEM INDONESIA Witono, Joko Ridho
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (920.495 KB) | DOI: 10.14203/beritabiologi.v9i2.2019

Abstract

There are 525 native palm species to Indonesia.Chromosome numbers of 61 Indonesian palm species have been reported,unfortunately, not all species were presented by their karyotype. Re-observation of palm chromosomes is needed, because mis identification of particular species and uncorrect chromosome counts might be happen in the past. Chromosome characteristics of some families, such as Poaceae and Onagraceae were proved useful for classification.On palms, almost all species within similar genera were known had similar chromosome numbers and different in chromosome forms and sizes.Trends to employ chromosome characteristics for palm classification is possible.Since, chromosome information on palm is limited,chromosome research has great challenge to do in the future.
GENETIC ANALYSIS OF SOME SPECIES OFPinanga (PALMAE) BY USING ISSR MARKERS Witono, Joko Ridho; Rondo, Katsuhiko
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i1.812

Abstract

A genetic analysis of 13 species of Pinanga (Palmae) was conducted by using Inter Simple Sequence Repeat (ISSR) markers. The markers were used in this study belonged to UBC primer set #9 (UBC 801-900) and each primer contains 15 to 22 mer nucleotides.Based on primer screening, nine UBC primers had clear and reproducible polymorphism bands. According to Dices and Jaccards similarity coefficients, cluster analysis by UPGMA among the 13 Pinanga species showed two clusters. Cluster A consisted of nine species: P. javana, P. arinasae, P. patula, P. salicifolia, P. coronata, P. scortechini, P. disticha, P. grandis and P. densiflora; and cluster B consisted of four species from five accessions: P. caesia, P. copelandi, P. rumphiana-1, P. rumphiana-2 and P. insignis. The genetic similarity among the 13 species of Pinanga had a correlation with their geographical distribution. In cluster A, all of the accessions are from Sundaland and the adjacent region of Thailand, whereas all of the accessions in cluster B were distributed in the Philippines, Wallacea, and the New Guinea regions. Possibly this genetic similarity was caused by their geographical history and the natural barriers between them. This is the early conclusion was conducted using genetic markers on Pinanga. Further studies such as sequencing (plastid and nuclear ribosomal DNA) and applying more accessions of Pinanga species from broader geographic distributions may provide a better understanding of the relationships. The 1SSR markers application is a simple and quick way to analyze genetic relationships because no prior sequence data is needed, a large number of markers can be generated, and the supplies and equipment required are minimal.
MODIFICATION OF DNA ISOLATION PROTOCOL FROM SILICA GEL DRIED-LEAF TISSUES OF Pinanga (PALMAE) Witono, Joko Ridho; Kondo, Katsuhiko
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (823.832 KB) | DOI: 10.14203/beritabiologi.v8i2.2034

Abstract

DNA isolation is an important issue in molecular fields especially for specific plant group such as Pinanga (Palmae). Typically,leaflets structure of Pinanga consists of shiny leaflets as common species of palms and mottled leaflets. Pinanga javana and P.coronata form commonly the two types of leaflets. The high quality of DNA of Pinanga with the shiny leaflet is readily purified and that with the mottled leaflet needed DNA purification due to the presence of high polyphenolic compound. Application of the silica binding method was recommended to purify the template DNA. Modified DNA-isolation protocol is relatively quick, simple,least expensive, minimum equipments and chemicals required, and suitable for PCR and endonucleases digestion reaction.
Co-Authors Ade Adriadi Agus Hikmat alex sumadijaya Ary PRIHARDHYANTO Keim Danang Wahyu Purnomo Didi Usmadi, Didi Farid Kuswantoro, Farid HIMMAH RUSTIAMI I PUTU GEDE P. DAMAYANTO Ina Erlinawati Inggit Puji Astuti Kondo, Katsuhiko LILIK BUDIPRASETYO Lulut D. Sulistyaningsih Mahat Magandhi Mar'atus, Saniyatun Marlina Ardiyani Marsanda, Firda Dwi Muhamad Syukur Mustaid Siregar Ni Putu Sri Asih R. Hendrian RAHMI YUNIANTI Rayhanissa Rondo, Katsuhiko Rosniati Apriani Risna Sri Wahyuni Syarifah Iis Aisyah Yayan Wahyu Candra Kusuma Yuzammi Zulkarnaen Zulkarnaen