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All Journal HAYATI Journal of Biosciences Buletin Kebun Raya Buitenzorg: Journal of Tropical Science
Mahat Magandhi
Pusat Konservasi Tumbuhan Kebun Raya Bogor – LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions

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Bauhinia pottsii G. Don var pottsii DARI KALIMANTAN DITEMUKAN KEMBALI Astuti, Inggit Puji; Magandhi, Mahat; Suratman, Maman; Maulida, Risa
Buletin Kebun Raya Vol 15, No 2 (2012): Buletin Kebun Raya Vol. 15 (2) Juli 2012
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Bauhinia is one of the largest genus in Caesalpiniaceae family and distributed in all tropical area.  One among 300 species of Bauhinia is Bauhinia pottsii G. Don.  This species is distributed from Southern Myanmar, Thailand, Cambodia, Malay Peninsular, Borneo, Sumatra, Jawa, Bali and Lesser Sunda islands. It was recorded that one specimen was originated from East Kalimantan and collected in 1925. During exploration in Kampung Temula, Kecamatan Nyuatan, Kabupaten Kutai Barat, East Kalimantan, Bauhinia pottsii G. Don var pottsii was found after about 87 years.  Bauhinia is one of the largest genus in Caesalpiniaceae family and distributed in all tropical area.  One among 300 species of Bauhinia is Bauhinia pottsii G. Don.  This species is distributed from Southern Myanmar, Thailand, Cambodia, Malay Peninsular, Borneo, Sumatra, Jawa, Bali and Lesser Sunda islands. It was recorded that one specimen was originated from East Kalimantan and collected in 1925. During exploration in Kampung Temula, Kecamatan Nyuatan, Kabupaten Kutai Barat, East Kalimantan, Bauhinia pottsii G. Don var pottsii was found after about 87 years.  
PENGEMBANGAN KOLEKSI TUMBUHAN KEBUN RAYA DAERAH DALAM KERANGKA STRATEGI KONSERVASI TUMBUHAN DI INDONESIA Purnomo, Danang Wahyu; Magandhi, Mahat; Kuswantoro, Farid; Risna, Rosniati Apriani; Witono, Joko Ridho
Buletin Kebun Raya Vol 18, No 2 (2015): Buletin Kebun Raya Vol. 18 (2) July 2015
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Sebagai bagian dari kebun raya dunia, Kebun Raya Indonesia (KRI) memiliki tanggung jawab untuk melaksanakan upaya konservasi tumbuhan yang ada di Indonesia. Hingga tahun 2010, empat kebun raya yang dikelola oleh LIPI hanya mampu mengkonservasi sekitar 21% dari seluruh tumbuhan terancam Indonesia. Oleh karena itu, diperlukan pengembangan Kebun Raya Daerah (KRD) untuk mengkonservasi tumbuhan pada tiap daerah di Indonesia. Makalah ini bertujuan untuk untuk mengetahui capaian KRD dalam konservasi tumbuhan dan menentukan strategi pengelolaan koleksi tumbuhan tiap daerah. Pada akhir 2012, KRI yang didukung KRD telah berhasil mengoleksi 24% dari tumbuhan terancam Indonesia versi IUCN Red List 2013, dan berhasil membudidayakan 25% termasuk koleksi pembibitan. Beberapa strategi untuk pengembangan koleksi KRD antara lain: penguatan sistem data base koleksi; pengembangan koleksi tumbuhan di setiap KRD dengan mengacu pada IUCN Red List; dan penentuan spesies prioritas untuk konservasi terkait dengan kebijakan dan kondisi alam di Indonesia.Kata kunci: koleksi, kebun raya daerah, IUCN Red List
Bauhinia pottsii G. Don var pottsii DARI KALIMANTAN DITEMUKAN KEMBALI Astuti, Inggit Puji; Magandhi, Mahat; Suratman, Maman; Maulida, Risa
Buletin Kebun Raya Vol 15, No 2 (2012): Buletin Kebun Raya Vol. 15 (2) July 2012
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Bauhinia pottsii G. Don tersebar dari Myanmar Selatan, Thailand, Kamboja dan Semenanjung Malaysia, Borneo, Sumatra, Jawa, Bali dan Kepulauan Sunda Kecil. Pendataan tentang persebaran jenis ini dengan menggunakan bahan penelitian spesimen herbarium dan ditemukan bahwa salah satu spesimen tersebut berasal dari Kalimantan Timur yang dikoleksikan tahun 1925.  Dalam kegiatan eksplorasi dan penelitian di Kampung Temula, Kecamatan Nyuatan, Kabupaten Kutai Barat, Kalimantan Timur Bauhinia pottsii G. Don var pottsii ini ditemukan kembali setelah kurang lebih 87 tahun.
Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) Magandhi, Mahat; Sobir; Matra, Deden Derajat; Wahyu, Yudiwanti; Sudarmono
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.132-139

Abstract

Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and cost-effectively sequences plant genomes, allowing for simple sequence repeat markers to be constructed in silico. This study aimed to design and evaluate novel, simple sequence repeat markers utilizing next-generation sequencing microsatellite data of D. testudinarius. A total of 20 sequences containing di-, tri-, tetra-, and penta-nucleotide motif repeats were selected and designed to generate primers using Primer3 online software. The PCR results of the twenty primers tested on D. testudinarius and 19 genomic DNA samples of species from the Malvales order showed successful amplification. These newly developed simple sequence repeat markers are crucial for genetic population analyses, determining population structure, and enhancing plant breeding programs and conservation strategies for D. testudinarius and its relative's germplasm resources.
Perbandingan Metode Ekstraksi DNA pada Tanaman Ulin (Eusideroxylon zwageri) Ridzqya, Salsabila Al Alya; Polosoro, Aqwin; Enggarini, Wening; Kusumanegara, Kusumawaty; Helmanto, Hendra; Magandhi, Mahat; Satyawan, Dani; Hadiarto, Toto; Yuniaty, Alice
Buitenzorg: Journal of Tropical Science Vol 1 No 1 (2024): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v1i1.7

Abstract

DNA isolation is a routine procedure in molecular analysis. The method of plant genome DNA extraction has been widely available throughout global laboratories. Several labs made some modifications to obtain optimal results. This research was aimed to analyse two types of DNA extraction methods for ironwood plant (Eusideroxylon zwageri), one tropical rain forest woody plant known for its high strength and durability. The results indicate that isolation DNA kit produced high DNA purity with lower concentrations while CTAB methods generated lower DNA purity with higher concentrations. This may be used as consideration of DNA isolation for downstream molecular analyses.   Keywords: DNA extraction, ironwood, CTAB, genomic DNA
Micromorphological Characterization and DNA Barcoding of Durio macrantha Kosterm. from the Bogor Botanical Garden Collection Magandhi, Mahat; Rusydina, Diyana Sabila; Mukaromah, Arnia Sari
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.16

Abstract

Durio macrantha is an endemic species first discovered in 1981 in Gunung Leuser National Park, North Sumatra, and has been conserved at the Bogor Botanical Garden since 1994. This study aims to characterize the micromorphology and DNA profile D. macrantha using DNA barcoding with rbcL and ITS gene markers. Sample of D. macrantha were collected from the Bogor Botanical Gardens for analysis. Micromorphological analysis revealed that the leaves exhibit three vein types (primary, secondary, and tertiary) with a pentagonal venation pattern and a tri-veinlet veinlet pattern. Additionally, eight types of trichomes were observed on the abaxial surface. Molecular characterization showed that the rbcL sequence was 563 bp long, with nucleotide composition T (27.9%), C (21.7%), A (27.9%), and G (22.6%). The ITS sequence was 960 bp long, with nucleotide composition T (15.4%), C (33.4%), A (19.0%), and G (32.2%). BLAST analysis of both rbcL and ITS genes revealed a high level of similarity between D. macrantha and D. zibethinus. This study provides fundamental data supporting the conservation and further research of D. macrantha, particularly in morphological and molecular aspects.
Co-Authors Alice Yuniaty Arnia Sari Mukaromah Danang Wahyu Purnomo Dani Satyawan Farid Kuswantoro, Farid Helmanto, Hendra Inggit Puji Astuti Inggit Puji Astuti Joko Ridho Witono Kusumanegara, Kusumawaty Maman Suratman Matra, Deden Derajat Polosoro, Aqwin Ridzqya, Salsabila Al Alya Risa Maulida Rosniati Apriani Risna Rusydina, Diyana Sabila Sobir Sobir Sudarmono Toto Hadiarto Wening Enggarini Yudiwanti Wahyu E. Kusumo