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Journal : Microbiology Indonesia

Bacterial Community Profiles in the Fluid of Four Pitcher Plant Species (Nepenthes spp.) Grown in a Nursery ANDREE SIEGARA; . YOGIARA
Microbiology Indonesia Vol. 3 No. 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (12.512 KB) | DOI: 10.5454/mi.3.3.3

Abstract

Nepenthes is one of the Indonesian tropical carnivorous plants. The plant has a pitcher-like structure containing fluid for digesting insects. There are many microorganisms growing in the pitcher fluid. Different species of pitcher plants and planting sites could also contribute either to the diversity or the abundance of microorganism inhabiting the pitcher fluid. To assess the bacterial community variation in the fluid of pitcher plants grown in a nursery, amplified ribosomal DNA restriction analysis (ARDRA) was used. Four specimens of N. gracilis, N. truncata, N. veitchii and N. bicalcarata were obtained from Suska Nursery, Ciderum Village, Caringin, Bogor, Indonesia. A total of 191 positive clones were analyzed by using ARDRA. A sum of 124 phylotypes was obtained, comprising 17 in N. gracilis, 7 in N. truncata, 45 in N. veitchii and 55 in N. bicalcarata. It is interesting to note that each specimen harbored unique phylotypes, meaning that no phylotypes generated from one specimen were found in any of other specimens.
Screening of Quorum Quenching Activity of Bacteria Isolated from Ant Lion Billy Christianto; . Yogiara
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (583.433 KB) | DOI: 10.5454/mi.5.1.8

Abstract

Bacterial intercellular communication or quorum sensing controls the pathogenesis of many medically important organisms. Therefore, it is important to isolate bacteria that can disintegrate the communication, in a process called quorum quenching. Bacteria from ant lion (Myrmeleon sp.) were grown on Luria agar, and approximately 1.85 x 109 CFU mL-1 was obtained. Eleven morphologically different colonies were screened for quorum quenching activity using wild type Chromobacterium violaceum as an indicator. Two isolates (Myr7 and MyrB) were found to possess quorum quenching activity. Isolates with quorum quenching activity were later identified employing 16S rRNA. Both isolates were similar to bacteria in the genus Aeromonas
Analysis of Bacterial Community Associated with Aaptos sp. from Rote and Seribu Islands EKOWATI CHASANAH; GINTUNG PATANTIS; ARIYANTI SUHITA DEWI; ENDAR MARRASKURANTO; HEDI INDRA JANUAR; STELLA STELLA; SUSAN SOKA; YOGIARA YOGIARA
Microbiology Indonesia Vol. 7 No. 1 (2013): March 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.473 KB) | DOI: 10.5454/mi.7.1.5

Abstract

Aaptos sp. is a marine sponge that could produce bioactive compounds such as aaptamin, aaptosin, and isoaaptamin which have activities as antitumor, antimicrobial, and antiviral. Community of bacteria associated with the sponge might correlate with production of those bioactive compounds and be affected  by  water environment where the sponge grow. The presence of anthropogenic stressor such as pollutans might become a burden to the waters where the biota grown and could affect the microbial biodiversity in the sponge and its active metabolite produced. The objective of this research was to analyze bacterial community associated with Aaptos sp. from Rote Island and Seribu Islands, using T-RFLP method. The results showed that bacterial community associated with Aaptos sp. from both sampling sites shared 40.81% similarity in which they were dominated by the same bacteria class of Actinobacteria, Flavobacteria, α-proteobacteria, δ-proteobacteria, and γ–proteobacteria. The bacteria collected from Rote island  were more highly distributed and diverse than those from Seribu Islands. A total of 23 classes of microorganism were identified in Rote Island waters, while in Seribu Islands was 14 classes of microorganism. The presence of Actinobacteria and Proteobacteria in Aaptos sp., is allegedly involved in the production of secondary metabolites.