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BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35, No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.

PURIFICATION AND PROPERTIES OF MANNANASE FROM Aspergillus ustus BL5 Thontowi, Ahmad; Rahmani, Nanik; Andriani, Ade; Yopi, -
Teknologi Indonesia Vol 37, No 1 (2014)
Publisher : LIPI Press

Strain of BL5 was reported as a mannanase producer. The purposes of this study are identification, purification and characterization of mannanase from BL5. The Internal Transcribed Spacer (ITS) regions analysis showed that BL5 strain have 93% similarity with Aspergillus ustus isolate UOA/HCPF 9236. An extracellular mannanase from the culture supernatant of a fungus A. ustus BL5 was purified. SDS-PAGE of the purified enzyme showed a single protein band of molecular mass 50 to 51 kDa. The mannanase exhibited optimum catalytic activity at pH 7.0 and 55C. The metal ions Ca2+, Cu2+ and SDS inhibited complete enzyme activity. The metal ion Mg2+ and EDTA increased complete enzyme activity. The value of Vmax = 5,88 ?mol mannose/min/ml and Kmax = 0.64 mg/ml. Mannanase of A. ustus BL5 be able to hydrolyzed porang mannan.

PECTINASE PRODUCTION BY ASPERGILLUS USTUS BL5 AT SOLID STATE FERMENTATION MEDIUM USING AGRICULTURAL BIOMASS Rahmani, Nanik; Andriani, Ade; Anggraini, Yufi Sara; Yopi, -
Teknologi Indonesia Vol 36, No 3 (2013)
Publisher : LIPI Press

This research showed that Indonesian agricultural biomasses, such as Siam orange peel, Medan orange peel, Durian peel and old tea leaves contain a pectin that are a potential material for use as a substrate for pectinase production. From the four types of biomasses used, Medan orange peel produced pectinase activity with the highest value equal to 1.28 U/mL. Optimization of fermentation conditions showed that Aspergillus ustus BL5 pectinase production were affected by the concentration of substrate, media pH, and temperature of the fermentation. Optimization of process showed that the optimum substrate concentration, pH and temperatuter were 10%, 4 and 40C with pectinase activity of 1.37 U/mL, 2.85 U/mL, and 1.92 U/mL respectively. Pectin content in the medium has a proportional relationship with the activity of the enzyme pectinase produced. The high content of pectin in Medanorange peel making pectinase enzyme activity produced on the substrate is also high.

BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35 No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.

PEMANFAATAN BAKTERI PROBIOTIK INDIGENUS DALAM PEMBUATAN KEJU LUNAK [Utilization of Indigenous Probiotic Bacteria in the Production of Soft Cheese] Fifi Afiati; - Yopi; Rarah R.A. Maheswari
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

PEMANFAATAN BAKTERI PROBIOTIK INDIGENUS DALAM PEMBUATAN KEJU LUNAK[Utilization of Indigenous Probiotic Bacteria in the Production of Soft Cheese]Fifi Afiati1)*, Yopi1) dan Rarah R.A. Maheswari2)1) Pusat Penelitian Bioteknologi LIPI, Jl. Raya Bogor Km 46, Cibinong, Bogor2) Departemen Ilmu dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor, Bogor Diterima 12 April 2013 / Disetujui 15 Februari 2014ABSTRACT Probiotic soft cheese containing lactic acid bacteria is one of functional food products. Three lactic acid bacteria namely Lactococcus lactis DSB 42 (LL-DSB42), Lactobacillus acidophilus RRM-01 (LA-RRM01) and Bifidobacterium longum RRM-01 (BL-RRM01) were used in the production of probiotic soft cheeses. Single culture (BL, LA, and LL) and mixed culture (BL-LA, BL-LL, LA-LL and BL-LA-LL) were used to produce diversified functional products. The preparation of probiotic soft cheese consists of pasteurization, addition of lactic acid bacterial culture (5%, v/v), addition of rennet, cutting the curd, scalding, draining and packaging. Soft cheese characteristics were analyzed physically (yield), chemically (pH, water content, crude protein, crude fat and ash) and microbiologically (lactic acid bacteria). The results showed that addition of lactic acid bacteria cultures significantly decreased the pH value (pH 5.10 to 5.79). The yield of probiotic soft cheese produced was in the range of 17.86-22.51% with water content of more than 55%. The fat and carbohydrate content of both cheeses of single and mixed cultures were significantly different (p<0.05) (fat content 5.1-7.4% for single culture and 4.0-9.3% for mix culture; carbohydrate content 11.6-17.7% for single culture and 4.6-12-2% for mix culture). The combination of all three starter cultures did not result in inhibition to each other, thus these combination were able to achieve the maximum number of 9.0 log10CFU g-1 on a single culture soft cheese and 9.8 log10CFU g-1 in mixed cultures soft cheese. In conclusion, soft cheeses with single culture (BL, LA, and LL) and mixed culture (BL-LA, BL-LL, LA-LL and BL-LA-LL) had excellent potential properties to be developed as probiotic foods.

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